| Basic Information | |
|---|---|
| Species | Linum usitatissimum |
| Cazyme ID | Lus10014382 |
| Family | GH13 |
| Protein Properties | Length: 924 Molecular Weight: 104905 Isoelectric Point: 5.9502 |
| Chromosome | Chromosome/Scaffold: 176 Start: 142342 End: 149532 |
| Description | starch branching enzyme 2.1 |
| View CDS | |
| External Links |
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| CAZyDB |
| Signature Domain Download full data set without filtering | |||
|---|---|---|---|
| Family | Start | End | Evalue |
| GH13 | 406 | 726 | 1.3e-29 |
| LPRIKKLGYNAVQIMAIQEHSYYASFGYHVTNFFAPSSRFGTPDDLKSLIDKAHELGLVVLMDIVHSHSSNNTLDGLNMFDGTDSHYFHSGARGHHMMWD SRLFNYGSWEVVRYLLSNARWWLDEYKFDGFRFDGVTSMMYTHHGLQMTFTGNYNEYFGFATDVDAVVYLMLVNDLIHGLFPEAITIGEDVSGMPTFCIP VQDGGVGFDYRLHMAIADKWIELLKKRDEDWRMGDIVHTLTNRRWLENCVCYAESHDQALVGDKTIAFWLMDKDMYDFMALDRPATPLIDRGIALHKMIR LITLGLGGEGYLNFMGNEFGH | |||
| Full Sequence |
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| Protein Sequence Length: 924 Download |
| MVYYAISGIR LPCVPSVHHN SLSASQLSIS LFLLQSRPPH FLRHHFAVFL LQEAPALLYS 60 LCPPQLRGVI FCCWTVVSFE WEFVANVLWK VLNRCLCVRK IGAASSSNDP EASSLTASTP 120 EKVLVPGAGA KPSSAIDVLV PDVNPSSSAD QLETPGILSE DSKALYEIPE SSTMIDGKNN 180 RILGKQGENA VFPDRRFTIG ERSIPPPGAG QRIYDIDPML SGFRQHLDYR YSQYKRIRAE 240 IDKFEGGLEE FSRGYEKFGF LRSETGITYR EWAPGATRAA LIGDFNNWNP NADVMTRNEF 300 GVWEIFLPNN ADGSSPIPHG SRVKIRMDTP SGLKDSIPAW IKYAVQAPGE IPYNGIYYDP 360 PEEEKYVFKH PQPDKPKSLR IYESHVGMSS TEPKINSYTS FRDDVLPRIK KLGYNAVQIM 420 AIQEHSYYAS FGYHVTNFFA PSSRFGTPDD LKSLIDKAHE LGLVVLMDIV HSHSSNNTLD 480 GLNMFDGTDS HYFHSGARGH HMMWDSRLFN YGSWEVVRYL LSNARWWLDE YKFDGFRFDG 540 VTSMMYTHHG LQMTFTGNYN EYFGFATDVD AVVYLMLVND LIHGLFPEAI TIGEDVSGMP 600 TFCIPVQDGG VGFDYRLHMA IADKWIELLK KRDEDWRMGD IVHTLTNRRW LENCVCYAES 660 HDQALVGDKT IAFWLMDKDM YDFMALDRPA TPLIDRGIAL HKMIRLITLG LGGEGYLNFM 720 GNEFGHPEWI DFPRGDQHLP NGKVVPGNNN SFDKCRRRFD LGDAEYLRYH GLQEFDRAMH 780 HLEGTYGFMT DIHQYISRKD EGDRVIVFER GNLVFVFNFH WNQSYSDYRV GCYKSGKYKI 840 VLDSDDALFG GFNRIDHNAE FFTSDDWYDD RPRSFMVYSP ARTAVVYALV EDTTEKAPAK 900 TESTVALVND NLKPPPASDS TQS* 960 |
| Functional Domains Download unfiltered results here | ||||||||
|---|---|---|---|---|---|---|---|---|
| Cdd ID | Domain | E-Value | Start | End | Length | Domain Description | ||
| PLN02960 | PLN02960 | 1.0e-10 | 223 | 307 | 91 | + alpha-amylase | ||
| PLN03244 | PLN03244 | 1.0e-137 | 317 | 889 | 580 | + alpha-amylase; Provisional | ||
| PLN02447 | PLN02447 | 0 | 187 | 915 | 733 | + 1,4-alpha-glucan-branching enzyme | ||
| cd11321 | AmyAc_bac_euk_BE | 0 | 362 | 777 | 417 | + Alpha amylase catalytic domain found in bacterial and eukaryotic branching enzymes. Branching enzymes (BEs) catalyze the formation of alpha-1,6 branch points in either glycogen or starch by cleavage of the alpha-1,4 glucosidic linkage yielding a non-reducing end oligosaccharide chain, and subsequent attachment to the alpha-1,6 position. By increasing the number of non-reducing ends, glycogen is more reactive to synthesis and digestion as well as being more soluble. This group includes bacterial and eukaryotic proteins. The Alpha-amylase family comprises the largest family of glycoside hydrolases (GH), with the majority of enzymes acting on starch, glycogen, and related oligo- and polysaccharides. These proteins catalyze the transformation of alpha-1,4 and alpha-1,6 glucosidic linkages with retention of the anomeric center. The protein is described as having 3 domains: A, B, C. A is a (beta/alpha) 8-barrel; B is a loop between the beta 3 strand and alpha 3 helix of A; C is the C-terminal extension characterized by a Greek key. The majority of the enzymes have an active site cleft found between domains A and B where a triad of catalytic residues (Asp, Glu and Asp) performs catalysis. Other members of this family have lost the catalytic activity as in the case of the human 4F2hc, or only have 2 residues that serve as the catalytic nucleophile and the acid/base, such as Thermus A4 beta-galactosidase with 2 Glu residues (GH42) and human alpha-galactosidase with 2 Asp residues (GH31). The family members are quite extensive and include: alpha amylase, maltosyltransferase, cyclodextrin glycotransferase, maltogenic amylase, neopullulanase, isoamylase, 1,4-alpha-D-glucan maltotetrahydrolase, 4-alpha-glucotransferase, oligo-1,6-glucosidase, amylosucrase, sucrose phosphorylase, and amylomaltase. | ||
| PLN02960 | PLN02960 | 0 | 317 | 890 | 578 | + alpha-amylase | ||
| Gene Ontology | |
|---|---|
| GO Term | Description |
| GO:0003824 | catalytic activity |
| GO:0004553 | hydrolase activity, hydrolyzing O-glycosyl compounds |
| GO:0005975 | carbohydrate metabolic process |
| GO:0043169 | cation binding |
| Annotations - NR Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| GenBank | AAT76444.1 | 0 | 66 | 917 | 8 | 854 | starch branching enzyme II [Vigna radiata] |
| GenBank | ABN05322.1 | 0 | 100 | 897 | 48 | 831 | starch branching enzyme II [Populus trichocarpa] |
| EMBL | CAA56319.1 | 0 | 4 | 905 | 3 | 859 | starch branching enzyme I [Pisum sativum] |
| RefSeq | XP_002326414.1 | 0 | 202 | 897 | 33 | 728 | predicted protein [Populus trichocarpa] |
| RefSeq | XP_002534111.1 | 0 | 92 | 895 | 41 | 842 | starch branching enzyme II, putative [Ricinus communis] |
| Annotations - PDB Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| PDB | 3amk_A | 0 | 213 | 896 | 13 | 699 | A Chain A, Structure Of The Starch Branching Enzyme I (Bei) From Oryza Sativa L |
| PDB | 3aml_A | 0 | 213 | 896 | 13 | 699 | A Chain A, Structure Of The Starch Branching Enzyme I (Bei) From Oryza Sativa L |
| PDB | 3vu2_B | 0 | 213 | 896 | 13 | 699 | A Chain A, Structure Of The Starch Branching Enzyme I (bei) Complexed With Maltopentaose From Oryza Sativa L |
| PDB | 3vu2_A | 0 | 213 | 896 | 13 | 699 | A Chain A, Structure Of The Starch Branching Enzyme I (bei) Complexed With Maltopentaose From Oryza Sativa L |
| PDB | 1m7x_D | 0 | 253 | 853 | 9 | 579 | A Chain A, The X-Ray Crystallographic Structure Of Branching Enzyme |
| Hydropathy |
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| EST Download unfiltered results here | ||||
|---|---|---|---|---|
| Hit | Length | Start | End | EValue |
| HO794536 | 711 | 207 | 917 | 0 |
| HO777638 | 657 | 261 | 917 | 0 |
| HO458123 | 395 | 496 | 890 | 0 |
| HO458123 | 291 | 207 | 495 | 0 |
| HO777638 | 47 | 216 | 262 | 0.0000000009 |
| Sequence Alignments (This image is cropped. Click for full image.) |
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