| Basic Information | |
|---|---|
| Species | Linum usitatissimum |
| Cazyme ID | Lus10017015 |
| Family | GH32 |
| Protein Properties | Length: 652 Molecular Weight: 73818.1 Isoelectric Point: 9.7482 |
| Chromosome | Chromosome/Scaffold: 315 Start: 376517 End: 381255 |
| Description | cell wall invertase 4 |
| View CDS | |
| External Links |
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| CAZyDB |
| Signature Domain Download full data set without filtering | |||
|---|---|---|---|
| Family | Start | End | Evalue |
| GH32 | 113 | 428 | 0 |
| HFQPPKHWINGPMYYNGFYHLFYQYNPKGAVWGNIVWAHSVSRDLVNWVALEPAIEATKPFDIGGCWSGSATILPNNKPILMYTGLDKNKRQLQNFAVPK NASDPFLREWVKPDFGNPFINPDPLVNASAFRDPTTAWFANGKWNTVVGGVYDMLGIGFLYQSKDFKKWVKTDHPLHAVWGTGMWECPDFFPVSVGGEEG GSDTYAAGDGVKYVFKVSLDLARFEYYTIGVYDKEKNKYVPDEGSVDSWAGLRYDYGNFYASKSFFDHKKNRRILWGWANESDSEDQDKAKGWAGIQLIP RKVWLDPSGKQLLQWP | |||
| Full Sequence |
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| Protein Sequence Length: 652 Download |
| MWSRSVMRYS LPTAAATTEV ARRKGRIREE SQRSVEGMST DRLARRLFST ATMKSMPVLE 60 KAERILGGDH SRRRXXXXII LAGDVAEGSH IVYSELQSVA VENVDATHRT AYHFQPPKHW 120 INGPMYYNGF YHLFYQYNPK GAVWGNIVWA HSVSRDLVNW VALEPAIEAT KPFDIGGCWS 180 GSATILPNNK PILMYTGLDK NKRQLQNFAV PKNASDPFLR EWVKPDFGNP FINPDPLVNA 240 SAFRDPTTAW FANGKWNTVV GGVYDMLGIG FLYQSKDFKK WVKTDHPLHA VWGTGMWECP 300 DFFPVSVGGE EGGSDTYAAG DGVKYVFKVS LDLARFEYYT IGVYDKEKNK YVPDEGSVDS 360 WAGLRYDYGN FYASKSFFDH KKNRRILWGW ANESDSEDQD KAKGWAGIQL IPRKVWLDPS 420 GKQLLQWPIE ELERLRNTHP VRLNHKQLKK GKLVRVDRIN AAQVDVEVTF SFKSLDKAEL 480 FDPKWADLPA QDVCNVRRSS VQGGLGPFGL LTLASSKLEE YTPVMFRVFK RGHNNKHVVL 540 FCSDASSSTL SRDPKMYKPS FAGYVNVDLS QNRKISVRSL IDHSVVESFA QGGKTVITSR 600 VYPTLAIQGR AHLFLFNNGT EEIKVEKLTA WNVRKPVMNV PLEEAVIKKF V* 660 |
| Functional Domains Download unfiltered results here | ||||||||
|---|---|---|---|---|---|---|---|---|
| Cdd ID | Domain | E-Value | Start | End | Length | Domain Description | ||
| TIGR01322 | scrB_fam | 2.0e-44 | 108 | 438 | 354 | + sucrose-6-phosphate hydrolase. [Energy metabolism, Biosynthesis and degradation of polysaccharides]. | ||
| COG1621 | SacC | 3.0e-55 | 91 | 604 | 549 | + Beta-fructosidases (levanase/invertase) [Carbohydrate transport and metabolism] | ||
| cd08996 | GH32_B_Fructosidase | 9.0e-83 | 119 | 431 | 328 | + Glycosyl hydrolase family 32, beta-fructosidases. Glycosyl hydrolase family GH32 cleaves sucrose into fructose and glucose via beta-fructofuranosidase activity, producing invert sugar that is a mixture of dextrorotatory D-glucose and levorotatory D-fructose, thus named invertase (EC 3.2.1.26). This family also contains other fructofuranosidases such as inulinase (EC 3.2.1.7), exo-inulinase (EC 3.2.1.80), levanase (EC 3.2.1.65), and transfructosidases such sucrose:sucrose 1-fructosyltransferase (EC 2.4.1.99), fructan:fructan 1-fructosyltransferase (EC 2.4.1.100), sucrose:fructan 6-fructosyltransferase (EC 2.4.1.10), fructan:fructan 6G-fructosyltransferase (EC 2.4.1.243) and levan fructosyltransferases (EC 2.4.1.-). These retaining enzymes (i.e. they retain the configuration at anomeric carbon atom of the substrate) catalyze hydrolysis in two steps involving a covalent glycosyl enzyme intermediate: an aspartate located close to the N-terminus acts as the catalytic nucleophile and a glutamate acts as the general acid/base; a conserved aspartate residue in the Arg-Asp-Pro (RDP) motif stabilizes the transition state. These enzymes are predicted to display a 5-fold beta-propeller fold as found for GH43 and CH68. The breakdown of sucrose is widely used as a carbon or energy source by bacteria, fungi, and plants. Invertase is used commercially in the confectionery industry, since fructose has a sweeter taste than sucrose and a lower tendency to crystallize. A common structural feature of all these enzymes is a 5-bladed beta-propeller domain, similar to GH43, that contains the catalytic acid and catalytic base. A long V-shaped groove, partially enclosed at one end, forms a single extended substrate-binding surface across the face of the propeller. | ||
| pfam00251 | Glyco_hydro_32N | 1.0e-137 | 113 | 428 | 328 | + Glycosyl hydrolases family 32 N-terminal domain. This domain corresponds to the N-terminal domain of glycosyl hydrolase family 32 which forms a five bladed beta propeller structure. | ||
| smart00640 | Glyco_32 | 4.0e-174 | 113 | 593 | 493 | + Glycosyl hydrolases family 32. | ||
| Annotations - NR Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| GenBank | ABY81288.1 | 0 | 46 | 643 | 3 | 583 | cell-wall invertase [Populus alba x Populus grandidentata] |
| GenBank | ABY81289.1 | 0 | 86 | 643 | 25 | 579 | cell-wall invertase [Populus alba x Populus grandidentata] |
| RefSeq | XP_002309419.1 | 0 | 87 | 643 | 25 | 577 | predicted protein [Populus trichocarpa] |
| RefSeq | XP_002530337.1 | 0 | 87 | 644 | 26 | 578 | Beta-fructofuranosidase, insoluble isoenzyme 3 precursor, putative [Ricinus communis] |
| RefSeq | XP_002530338.1 | 0 | 79 | 644 | 19 | 573 | Beta-fructofuranosidase, insoluble isoenzyme 1 precursor, putative [Ricinus communis] |
| Annotations - PDB Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| PDB | 2ac1_A | 0 | 103 | 633 | 3 | 535 | A Chain A, Crystal Structure Of A Cell-Wall Invertase From Arabidopsis Thaliana |
| PDB | 2qqw_A | 0 | 108 | 633 | 4 | 531 | A Chain A, Crystal Structure Of A Cell-Wall Invertase (D23a) From Arabidopsis Thaliana In Complex With Sucrose |
| PDB | 2xqr_K | 0 | 108 | 633 | 4 | 531 | B Chain B, Crystal Structure Of Plant Cell Wall Invertase In Complex With A Specific Protein Inhibitor |
| PDB | 2xqr_I | 0 | 108 | 633 | 4 | 531 | B Chain B, Crystal Structure Of Plant Cell Wall Invertase In Complex With A Specific Protein Inhibitor |
| PDB | 2xqr_G | 0 | 108 | 633 | 4 | 531 | B Chain B, Crystal Structure Of Plant Cell Wall Invertase In Complex With A Specific Protein Inhibitor |
| Hydropathy |
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| EST Download unfiltered results here | ||||
|---|---|---|---|---|
| Hit | Length | Start | End | EValue |
| CT842376 | 564 | 89 | 639 | 0 |
| DY298870 | 334 | 79 | 407 | 0 |
| DY273985 | 352 | 125 | 473 | 0 |
| DY292291 | 347 | 79 | 417 | 0 |
| FC881182 | 306 | 125 | 427 | 0 |
| Sequence Alignments (This image is cropped. Click for full image.) |
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