| Basic Information | |
|---|---|
| Species | Linum usitatissimum |
| Cazyme ID | Lus10024605 |
| Family | GH32 |
| Protein Properties | Length: 651 Molecular Weight: 72387.7 Isoelectric Point: 6.725 |
| Chromosome | Chromosome/Scaffold: 349 Start: 252712 End: 255575 |
| Description | Glycosyl hydrolases family 32 protein |
| View CDS | |
| External Links |
|---|
| CAZyDB |
| Signature Domain Download full data set without filtering | |||
|---|---|---|---|
| Family | Start | End | Evalue |
| GH32 | 127 | 447 | 0 |
| HFQPEKNWMNGPLYYKGWYHFFYQYNPNAAVWGDIVWGHAVSHDLIHWLHLPLAMVANQWYDVNGVWTGSATILPDGKVVMLYTGSTNESVQVQNLAYPA DPSDPLLLEWVKHPANPVLVPPPGIHYKDFRDPTTAWRTSEGKWRITIGSKECGTKYNRTGVALIYDTEDFINYDLKPGFLHGVPDTGMWECVDFYPVSV AGGNGLDTSANGPGVRHVVKASMDDDRHDYYAIGTYDEAKSKWFPDNPEIDVGIGIRYDYGIFYASKTFYDQSKQRRILWGWIGESDSEVADIRKGWASL QSIPRTVVLDTKTGSNLLQWP | |||
| Full Sequence |
|---|
| Protein Sequence Length: 651 Download |
| MADHEQPFLL PVTSGSVNPE HAPTSDAAVG RSNKRILLLA GFAGIFIVCL VFTGGYDKSG 60 AVVKFPELVG TSVINSGPEK ISRGKSAGVS EKSNLLHRRQ TNLIGGEKEE AFPWNNSMLS 120 WQRTAFHFQP EKNWMNGPLY YKGWYHFFYQ YNPNAAVWGD IVWGHAVSHD LIHWLHLPLA 180 MVANQWYDVN GVWTGSATIL PDGKVVMLYT GSTNESVQVQ NLAYPADPSD PLLLEWVKHP 240 ANPVLVPPPG IHYKDFRDPT TAWRTSEGKW RITIGSKECG TKYNRTGVAL IYDTEDFINY 300 DLKPGFLHGV PDTGMWECVD FYPVSVAGGN GLDTSANGPG VRHVVKASMD DDRHDYYAIG 360 TYDEAKSKWF PDNPEIDVGI GIRYDYGIFY ASKTFYDQSK QRRILWGWIG ESDSEVADIR 420 KGWASLQSIP RTVVLDTKTG SNLLQWPVEE TESLRLRSKE FNNVAVKPGS IVPLELEAAT 480 QLDIEAEFEL DPKAVQTAAE SNEDFKCTGS RNRGALGPFG LLVLADDTQT EQTPVYFYVV 540 KGLNGTLRTH FCTDQMRSSL ANDVNKQVYG SSVPVLEGEK FKVRVLVDHS IVESFGQGGR 600 VVISSRVYPT RAIYGAAKVF LFNRGKAGDV RGSVKVWQMN SAFIRPYSKR * 660 |
| Functional Domains Download unfiltered results here | ||||||||
|---|---|---|---|---|---|---|---|---|
| Cdd ID | Domain | E-Value | Start | End | Length | Domain Description | ||
| TIGR01322 | scrB_fam | 1.0e-50 | 123 | 609 | 506 | + sucrose-6-phosphate hydrolase. [Energy metabolism, Biosynthesis and degradation of polysaccharides]. | ||
| COG1621 | SacC | 9.0e-70 | 123 | 611 | 507 | + Beta-fructosidases (levanase/invertase) [Carbohydrate transport and metabolism] | ||
| cd08996 | GH32_B_Fructosidase | 5.0e-91 | 133 | 450 | 326 | + Glycosyl hydrolase family 32, beta-fructosidases. Glycosyl hydrolase family GH32 cleaves sucrose into fructose and glucose via beta-fructofuranosidase activity, producing invert sugar that is a mixture of dextrorotatory D-glucose and levorotatory D-fructose, thus named invertase (EC 3.2.1.26). This family also contains other fructofuranosidases such as inulinase (EC 3.2.1.7), exo-inulinase (EC 3.2.1.80), levanase (EC 3.2.1.65), and transfructosidases such sucrose:sucrose 1-fructosyltransferase (EC 2.4.1.99), fructan:fructan 1-fructosyltransferase (EC 2.4.1.100), sucrose:fructan 6-fructosyltransferase (EC 2.4.1.10), fructan:fructan 6G-fructosyltransferase (EC 2.4.1.243) and levan fructosyltransferases (EC 2.4.1.-). These retaining enzymes (i.e. they retain the configuration at anomeric carbon atom of the substrate) catalyze hydrolysis in two steps involving a covalent glycosyl enzyme intermediate: an aspartate located close to the N-terminus acts as the catalytic nucleophile and a glutamate acts as the general acid/base; a conserved aspartate residue in the Arg-Asp-Pro (RDP) motif stabilizes the transition state. These enzymes are predicted to display a 5-fold beta-propeller fold as found for GH43 and CH68. The breakdown of sucrose is widely used as a carbon or energy source by bacteria, fungi, and plants. Invertase is used commercially in the confectionery industry, since fructose has a sweeter taste than sucrose and a lower tendency to crystallize. A common structural feature of all these enzymes is a 5-bladed beta-propeller domain, similar to GH43, that contains the catalytic acid and catalytic base. A long V-shaped groove, partially enclosed at one end, forms a single extended substrate-binding surface across the face of the propeller. | ||
| pfam00251 | Glyco_hydro_32N | 4.0e-148 | 127 | 447 | 331 | + Glycosyl hydrolases family 32 N-terminal domain. This domain corresponds to the N-terminal domain of glycosyl hydrolase family 32 which forms a five bladed beta propeller structure. | ||
| smart00640 | Glyco_32 | 0 | 127 | 599 | 485 | + Glycosyl hydrolases family 32. | ||
| Gene Ontology | |
|---|---|
| GO Term | Description |
| GO:0004564 | beta-fructofuranosidase activity |
| GO:0004575 | sucrose alpha-glucosidase activity |
| Annotations - NR Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| DDBJ | BAB82419.1 | 0 | 78 | 647 | 79 | 644 | acid invertase [Citrus unshiu] |
| DDBJ | BAF34362.1 | 0 | 78 | 647 | 79 | 644 | beta-fructofuranosidase [Citrus sinensis] |
| Swiss-Prot | P29001 | 0 | 3 | 647 | 2 | 641 | INVA_PHAAU RecName: Full=Acid beta-fructofuranosidase; AltName: Full=Acid sucrose hydrolase; AltName: Full=Acid invertase; Short=AI; AltName: Full=Vacuolar invertase; Contains: RecName: Full=Acid beta-fructofuranosidase 30 kDa subunit; Contains: RecName: Full=Acid beta-fructofuranosidase 38 kDa subunit; Flags: Precursor |
| RefSeq | XP_002303519.1 | 0 | 78 | 650 | 94 | 660 | predicted protein [Populus trichocarpa] |
| RefSeq | XP_002532576.1 | 0 | 81 | 647 | 85 | 645 | Acid beta-fructofuranosidase precursor, putative [Ricinus communis] |
| Annotations - PDB Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| PDB | 3ugh_B | 0 | 112 | 649 | 8 | 546 | A Chain A, Crystal Structure Of Medicago Truncatula Ugt71g1 Complexed With Udp-Glucose |
| PDB | 3ugh_A | 0 | 112 | 649 | 8 | 546 | A Chain A, Crystal Structure Of Medicago Truncatula Ugt71g1 Complexed With Udp-Glucose |
| PDB | 3ugg_B | 0 | 112 | 649 | 8 | 546 | A Chain A, Crystal Structure Of Medicago Truncatula Ugt71g1 Complexed With Udp-Glucose |
| PDB | 3ugg_A | 0 | 112 | 649 | 8 | 546 | A Chain A, Crystal Structure Of Medicago Truncatula Ugt71g1 Complexed With Udp-Glucose |
| PDB | 3ugf_B | 0 | 112 | 649 | 8 | 546 | A Chain A, Crystal Structure Of A 6-Sst6-Sft From Pachysandra Terminalis |
