| Basic Information | |
|---|---|
| Species | Linum usitatissimum |
| Cazyme ID | Lus10028922 |
| Family | GH32 |
| Protein Properties | Length: 648 Molecular Weight: 71629 Isoelectric Point: 6.4598 |
| Chromosome | Chromosome/Scaffold: 540 Start: 877935 End: 880368 |
| Description | Glycosyl hydrolases family 32 protein |
| View CDS | |
| External Links |
|---|
| CAZyDB |
| Signature Domain Download full data set without filtering | |||
|---|---|---|---|
| Family | Start | End | Evalue |
| GH32 | 126 | 441 | 0 |
| HFQPEKNWMNGPLFYKGWYHFFYQYNPNAPVWGDIVWGHAVSRDLIHWLHLPLAMVADQWYDVNGVWTGSATILPNGNLVMLYTGSTNESVQVQNLAYPA DHNDPLLLNWVKYPGNPVLFPPLGIHFKDFRDPTTAWRTPEGKWRITIGSKVNKTGIALVYDTDDFINFDLKDGILHGVPGTGMWECVDFYPVAVDGRNG MDTSANGAGVKHVMKTSLDDDRHDYYAIGSYDEVNAKWVPDNPEIDVGIGIRYDYGIFYASKTFYDQSKQRRVLWGWIGESDSEAADVRKGWASLQGIPR TVVLDTKTGSNLLQWP | |||
| Full Sequence |
|---|
| Protein Sequence Length: 648 Download |
| MADNKQPFLP VTGGEPSKNH APSSGSRKKL LLAVFGGIFV VCLILAGGGY DKSGLVKSAR 60 FGSDLDEVLV GPAGSAIRSA VSRGKSAGVS EKANLPEHQL LRGENGVTEE FPWNNSMLSW 120 QRTAFHFQPE KNWMNGPLFY KGWYHFFYQY NPNAPVWGDI VWGHAVSRDL IHWLHLPLAM 180 VADQWYDVNG VWTGSATILP NGNLVMLYTG STNESVQVQN LAYPADHNDP LLLNWVKYPG 240 NPVLFPPLGI HFKDFRDPTT AWRTPEGKWR ITIGSKVNKT GIALVYDTDD FINFDLKDGI 300 LHGVPGTGMW ECVDFYPVAV DGRNGMDTSA NGAGVKHVMK TSLDDDRHDY YAIGSYDEVN 360 AKWVPDNPEI DVGIGIRYDY GIFYASKTFY DQSKQRRVLW GWIGESDSEA ADVRKGWASL 420 QGIPRTVVLD TKTGSNLLQW PVEEADKLRL RSQEFNNVVA KPGSVVAIKV ESATQLDVVA 480 EFELDKEAVE KAVESDEEYK CSSSKGAAQR GALGPFGLLV LSEDTLSEQT PVYFYVVKGK 540 NGTLRTSFCT DQSRSSLAND VNKKLYGSFV PVLEGEKFKV RMLVDHSIIE SFAQGGRSVI 600 TSRVYPTKAI YGAARLFLFN NATEAGVTAS LKVWQMNSAF IRPYPNL* 660 |
| Functional Domains Download unfiltered results here | ||||||||
|---|---|---|---|---|---|---|---|---|
| Cdd ID | Domain | E-Value | Start | End | Length | Domain Description | ||
| TIGR01322 | scrB_fam | 7.0e-53 | 122 | 606 | 508 | + sucrose-6-phosphate hydrolase. [Energy metabolism, Biosynthesis and degradation of polysaccharides]. | ||
| COG1621 | SacC | 6.0e-69 | 122 | 607 | 503 | + Beta-fructosidases (levanase/invertase) [Carbohydrate transport and metabolism] | ||
| cd08996 | GH32_B_Fructosidase | 8.0e-94 | 132 | 444 | 322 | + Glycosyl hydrolase family 32, beta-fructosidases. Glycosyl hydrolase family GH32 cleaves sucrose into fructose and glucose via beta-fructofuranosidase activity, producing invert sugar that is a mixture of dextrorotatory D-glucose and levorotatory D-fructose, thus named invertase (EC 3.2.1.26). This family also contains other fructofuranosidases such as inulinase (EC 3.2.1.7), exo-inulinase (EC 3.2.1.80), levanase (EC 3.2.1.65), and transfructosidases such sucrose:sucrose 1-fructosyltransferase (EC 2.4.1.99), fructan:fructan 1-fructosyltransferase (EC 2.4.1.100), sucrose:fructan 6-fructosyltransferase (EC 2.4.1.10), fructan:fructan 6G-fructosyltransferase (EC 2.4.1.243) and levan fructosyltransferases (EC 2.4.1.-). These retaining enzymes (i.e. they retain the configuration at anomeric carbon atom of the substrate) catalyze hydrolysis in two steps involving a covalent glycosyl enzyme intermediate: an aspartate located close to the N-terminus acts as the catalytic nucleophile and a glutamate acts as the general acid/base; a conserved aspartate residue in the Arg-Asp-Pro (RDP) motif stabilizes the transition state. These enzymes are predicted to display a 5-fold beta-propeller fold as found for GH43 and CH68. The breakdown of sucrose is widely used as a carbon or energy source by bacteria, fungi, and plants. Invertase is used commercially in the confectionery industry, since fructose has a sweeter taste than sucrose and a lower tendency to crystallize. A common structural feature of all these enzymes is a 5-bladed beta-propeller domain, similar to GH43, that contains the catalytic acid and catalytic base. A long V-shaped groove, partially enclosed at one end, forms a single extended substrate-binding surface across the face of the propeller. | ||
| pfam00251 | Glyco_hydro_32N | 2.0e-150 | 126 | 441 | 327 | + Glycosyl hydrolases family 32 N-terminal domain. This domain corresponds to the N-terminal domain of glycosyl hydrolase family 32 which forms a five bladed beta propeller structure. | ||
| smart00640 | Glyco_32 | 0 | 126 | 596 | 484 | + Glycosyl hydrolases family 32. | ||
| Gene Ontology | |
|---|---|
| GO Term | Description |
| GO:0004564 | beta-fructofuranosidase activity |
| GO:0004575 | sucrose alpha-glucosidase activity |
| Annotations - NR Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| GenBank | ACT21538.1 | 0 | 81 | 645 | 8 | 572 | acid invertase [Vigna radiata] |
| DDBJ | BAF34362.1 | 0 | 82 | 646 | 86 | 646 | beta-fructofuranosidase [Citrus sinensis] |
| Swiss-Prot | P29001 | 0 | 3 | 645 | 2 | 642 | INVA_PHAAU RecName: Full=Acid beta-fructofuranosidase; AltName: Full=Acid sucrose hydrolase; AltName: Full=Acid invertase; Short=AI; AltName: Full=Vacuolar invertase; Contains: RecName: Full=Acid beta-fructofuranosidase 30 kDa subunit; Contains: RecName: Full=Acid beta-fructofuranosidase 38 kDa subunit; Flags: Precursor |
| RefSeq | XP_002303519.1 | 0 | 64 | 646 | 75 | 659 | predicted protein [Populus trichocarpa] |
| RefSeq | XP_002532576.1 | 0 | 77 | 647 | 81 | 648 | Acid beta-fructofuranosidase precursor, putative [Ricinus communis] |
| Annotations - PDB Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| PDB | 3ugh_B | 0 | 111 | 644 | 8 | 544 | A Chain A, Crystal Structure Of Medicago Truncatula Ugt71g1 Complexed With Udp-Glucose |
| PDB | 3ugh_A | 0 | 111 | 644 | 8 | 544 | A Chain A, Crystal Structure Of Medicago Truncatula Ugt71g1 Complexed With Udp-Glucose |
| PDB | 3ugg_B | 0 | 111 | 644 | 8 | 544 | A Chain A, Crystal Structure Of Medicago Truncatula Ugt71g1 Complexed With Udp-Glucose |
| PDB | 3ugg_A | 0 | 111 | 644 | 8 | 544 | A Chain A, Crystal Structure Of Medicago Truncatula Ugt71g1 Complexed With Udp-Glucose |
| PDB | 3ugf_B | 0 | 111 | 644 | 8 | 544 | A Chain A, Crystal Structure Of A 6-Sst6-Sft From Pachysandra Terminalis |
