| Basic Information | |
|---|---|
| Species | Linum usitatissimum |
| Cazyme ID | Lus10030278 |
| Family | GH32 |
| Protein Properties | Length: 562 Molecular Weight: 63099.2 Isoelectric Point: 6.3519 |
| Chromosome | Chromosome/Scaffold: 217 Start: 543606 End: 545745 |
| Description | Glycosyl hydrolases family 32 protein |
| View CDS | |
| External Links |
|---|
| CAZyDB |
| Signature Domain Download full data set without filtering | |||
|---|---|---|---|
| Family | Start | End | Evalue |
| GH32 | 112 | 430 | 0 |
| HFQSEKNWMNDPNGPLYYKGWYHFFYQYNPFSAVWGDIAWGHAVSKDLVRWVHLPLAMVPDQWYDVNGVWTGSATILPDGKIVMLYTGSTNDSVQVQCLA YPANHDDPLLLDWVKYSGNPVLVPPPGIGIKDFRDPTTAWMTSEGKWRITVGSKIHRTGIALVYDTEDFIHYEQKPVPLHGVPGTGMWECVDFYPVSMTG SDGLDTSANSPDVKHVMKASLDDDRHDYYALGIYDDQTGKWTPDNSEIDVGIGIRYDYGVFYASKTFYDESKKRRVLWGWVGESDSESSDVKKGWACLQG FPRTVSMDIKTKTNLIQWP | |||
| Full Sequence |
|---|
| Protein Sequence Length: 562 Download |
| MADQRTPILP VADPDYIPIP DDSTSPGRVS TRNRSKKLLL FSLSCGLALV WVILGNGSDH 60 STDIGHTPST AAKTTSRGKH DGVSEKSNGQ QSLFRDYDWE NKTLSWQRTA FHFQSEKNWM 120 NDPNGPLYYK GWYHFFYQYN PFSAVWGDIA WGHAVSKDLV RWVHLPLAMV PDQWYDVNGV 180 WTGSATILPD GKIVMLYTGS TNDSVQVQCL AYPANHDDPL LLDWVKYSGN PVLVPPPGIG 240 IKDFRDPTTA WMTSEGKWRI TVGSKIHRTG IALVYDTEDF IHYEQKPVPL HGVPGTGMWE 300 CVDFYPVSMT GSDGLDTSAN SPDVKHVMKA SLDDDRHDYY ALGIYDDQTG KWTPDNSEID 360 VGIGIRYDYG VFYASKTFYD ESKKRRVLWG WVGESDSESS DVKKGWACLQ GFPRTVSMDI 420 KTKTNLIQWP VEEIETLRRG KRESRSSLAT DVSKVIHGSY VPTLEGEKIS VRVLVDHSIV 480 ESFAQGGRSV ITARVYPTMA IYGNAKVFLF NNATEAAVKA SVQVWEMGSA YEPVSDSVRR 540 GPRLGFAVLF YMLYYLVLMF Y* 600 |
| Functional Domains Download unfiltered results here | ||||||||
|---|---|---|---|---|---|---|---|---|
| Cdd ID | Domain | E-Value | Start | End | Length | Domain Description | ||
| smart00640 | Glyco_32 | 5.0e-7 | 442 | 487 | 46 | + Glycosyl hydrolases family 32. | ||
| COG1621 | SacC | 2.0e-63 | 108 | 443 | 347 | + Beta-fructosidases (levanase/invertase) [Carbohydrate transport and metabolism] | ||
| cd08996 | GH32_B_Fructosidase | 6.0e-94 | 118 | 433 | 322 | + Glycosyl hydrolase family 32, beta-fructosidases. Glycosyl hydrolase family GH32 cleaves sucrose into fructose and glucose via beta-fructofuranosidase activity, producing invert sugar that is a mixture of dextrorotatory D-glucose and levorotatory D-fructose, thus named invertase (EC 3.2.1.26). This family also contains other fructofuranosidases such as inulinase (EC 3.2.1.7), exo-inulinase (EC 3.2.1.80), levanase (EC 3.2.1.65), and transfructosidases such sucrose:sucrose 1-fructosyltransferase (EC 2.4.1.99), fructan:fructan 1-fructosyltransferase (EC 2.4.1.100), sucrose:fructan 6-fructosyltransferase (EC 2.4.1.10), fructan:fructan 6G-fructosyltransferase (EC 2.4.1.243) and levan fructosyltransferases (EC 2.4.1.-). These retaining enzymes (i.e. they retain the configuration at anomeric carbon atom of the substrate) catalyze hydrolysis in two steps involving a covalent glycosyl enzyme intermediate: an aspartate located close to the N-terminus acts as the catalytic nucleophile and a glutamate acts as the general acid/base; a conserved aspartate residue in the Arg-Asp-Pro (RDP) motif stabilizes the transition state. These enzymes are predicted to display a 5-fold beta-propeller fold as found for GH43 and CH68. The breakdown of sucrose is widely used as a carbon or energy source by bacteria, fungi, and plants. Invertase is used commercially in the confectionery industry, since fructose has a sweeter taste than sucrose and a lower tendency to crystallize. A common structural feature of all these enzymes is a 5-bladed beta-propeller domain, similar to GH43, that contains the catalytic acid and catalytic base. A long V-shaped groove, partially enclosed at one end, forms a single extended substrate-binding surface across the face of the propeller. | ||
| pfam00251 | Glyco_hydro_32N | 2.0e-147 | 112 | 430 | 327 | + Glycosyl hydrolases family 32 N-terminal domain. This domain corresponds to the N-terminal domain of glycosyl hydrolase family 32 which forms a five bladed beta propeller structure. | ||
| smart00640 | Glyco_32 | 3.0e-148 | 112 | 441 | 337 | + Glycosyl hydrolases family 32. | ||
| Gene Ontology | |
|---|---|
| GO Term | Description |
| GO:0004564 | beta-fructofuranosidase activity |
| GO:0004575 | sucrose alpha-glucosidase activity |
| Annotations - NR Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| DDBJ | BAF35859.1 | 0 | 1 | 488 | 1 | 502 | soluble acid invertase [Pyrus pyrifolia var. culta] |
| DDBJ | BAF35859.1 | 7e-30 | 443 | 559 | 557 | 680 | soluble acid invertase [Pyrus pyrifolia var. culta] |
| RefSeq | XP_002303519.1 | 0 | 1 | 438 | 1 | 462 | predicted protein [Populus trichocarpa] |
| RefSeq | XP_002303519.1 | 2e-31 | 443 | 531 | 565 | 653 | predicted protein [Populus trichocarpa] |
| RefSeq | XP_002532576.1 | 0 | 73 | 443 | 83 | 455 | Acid beta-fructofuranosidase precursor, putative [Ricinus communis] |
| Annotations - PDB Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| PDB | 3ugh_B | 0 | 97 | 486 | 8 | 391 | A Chain A, I-band Fragment I65-i70 From Titin |
| PDB | 3ugh_B | 5e-31 | 443 | 531 | 452 | 540 | A Chain A, I-band Fragment I65-i70 From Titin |
| PDB | 3ugh_A | 0 | 97 | 486 | 8 | 391 | A Chain A, I-band Fragment I65-i70 From Titin |
| PDB | 3ugh_A | 5e-31 | 443 | 531 | 452 | 540 | A Chain A, I-band Fragment I65-i70 From Titin |
| PDB | 3ugg_B | 0 | 97 | 486 | 8 | 391 | A Chain A, I-band Fragment I65-i70 From Titin |
