| Basic Information | |
|---|---|
| Species | Linum usitatissimum |
| Cazyme ID | Lus10030793 |
| Family | CBM45 |
| Protein Properties | Length: 1202 Molecular Weight: 133952 Isoelectric Point: 6.9981 |
| Chromosome | Chromosome/Scaffold: 373 Start: 566746 End: 574468 |
| Description | alpha-amylase-like 3 |
| View CDS | |
| External Links |
|---|
| CAZyDB |
| Signature Domain Download full data set without filtering | |||
|---|---|---|---|
 | |||
| Family | Start | End | Evalue |
| CBM45 | 315 | 391 | 1e-23 |
| LHWGVCRDDAKNWEIPSGPHPPETIIFKERALRTKLQAKDDGRGCAGLFTLDEDIAGFLFVLKLNDNTWFKCMGNDF | |||
| CBM45 | 133 | 216 | 9.2e-27 |
| LHWGVSYVNDSGSEWDQPPKHMRPPGSVPVKNYAIDTPLKKPSVDDLFYEVKIDIDPKSAIASINFVLKDEETGSWYQHKGRDF | |||
| GH13 | 532 | 820 | 1.2e-35 |
| DKAEDIASLGFTVIWLPPPTESVAPGGYMPKGLYNLNSRYGRIDELKDLVGRLHDSGIKVLGDAVLNHRCAHYQNKNGIWNIFGGRLSWDDRAVVADDPH FQGRGNKSSGDNFHAAPNIDHSQDFVRKDIKEWMGWLREEIGYDGWRLDFVRGFWGGYVKDYLDATNPYFAVGEYWDSLSYTYGEMDHNQDAHRQRIIDW INATNGSAGAFDVTTKGVLHAALEKCDYWRLADEKGKPPGVVGWWPSRAVTFVENHDTGSTQGHWRFPGGKEMQGYAYILTHPGTPAVF | |||
| Full Sequence |
|---|
| Protein Sequence Length: 1202 Download |
| MSSVTTVEPI LRCSHWREIH GSLKATKLCC STSSLLNCLR QKRFVRANFG KCKLPVGRAK 60 APAMIRASRS SSSSGTALPE TFVSSGDVFF RQSFPVTRTE TVDGKIFVRV DQSNGKGDWQ 120 LTVGCSLPGK WILHWGVSYV NDSGSEWDQP PKHMRPPGSV PVKNYAIDTP LKKPSVDDLF 180 YEVKIDIDPK SAIASINFVL KDEETGSWYQ HKGRDFKVPL VDYLLDDGNF SGSNSFNIWP 240 GPFFTNILLK SERQNNVSEL NDSKTEDRQQ EGIYEEQPFT KHTTLENSVT VLVRTCPETS 300 KNLLYLETDL PGVVLHWGVC RDDAKNWEIP SGPHPPETII FKERALRTKL QAKDDGRGCA 360 GLFTLDEDIA GFLFVLKLND NTWFKCMGND FYIPLSSSSR LLAQPGQKHS EDAPASGKAL 420 DADQGSGTAF TDGIIKEIRN LVSGISFENS RKVKTKEAQE SILQEIEKLA AEAYSIFRSS 480 IPTFKEEVVL EEELEKAPPK KCSGTGSGYE ILLQGFNWES HKSGRWYMDL KDKAEDIASL 540 GFTVIWLPPP TESVAPGGYM PKGLYNLNSR YGRIDELKDL VGRLHDSGIK VLGDAVLNHR 600 CAHYQNKNGI WNIFGGRLSW DDRAVVADDP HFQGRGNKSS GDNFHAAPNI DHSQDFVRKD 660 IKEWMGWLRE EIGYDGWRLD FVRGFWGGYV KDYLDATNPY FAVGEYWDSL SYTYGEMDHN 720 QDAHRQRIID WINATNGSAG AFDVTTKGVL HAALEKCDYW RLADEKGKPP GVVGWWPSRA 780 VTFVENHDTG STQGHWRFPG GKEMQGYAYI LTHPGTPAVF FDHIYSSNYR SEIAALIAFR 840 NQHKLHCRSV VKISKAVNDV YAAIIDDKVA MKIGPGHFEP PKGPRNWSLA LEGRDYKVPE 900 FFRFSAQPWP CKLHSVPDFF YSAEQVRFAT DHSASGAVSL ILIFHSSTGY FGTVLVKKGK 960 LSDLIGELQT LVNGIEGGDT DGESGHADAL AQQITVLNGN GRSGNLTALL IPGAVVGVIG 1020 YGYMWWKGMK FSDLMYVTKR NMTAAAAKLT KHLEDVTGAL AKAKEHLNQR IKGLDDKMDV 1080 QKEISKAIQN DVNTINENIQ LLDSDLFDLQ RIVSGLDGKI GSLENKQDLA NLGITYLCHF 1140 AQGKKLAMPK ELKDHVASGK NRITFHQDAG GLKALTDDSF CKLVTENLDL PQVFQRSMLS 1200 R* |
| Functional Domains Download unfiltered results here | ||||||||
|---|---|---|---|---|---|---|---|---|
 | ||||||||
| Cdd ID | Domain | E-Value | Start | End | Length | Domain Description | ||
| PRK09441 | PRK09441 | 2.0e-47 | 510 | 843 | 420 | + cytoplasmic alpha-amylase; Reviewed | ||
| PLN00196 | PLN00196 | 3.0e-131 | 510 | 900 | 403 | + alpha-amylase; Provisional | ||
| cd11314 | AmyAc_arch_bac_plant_AmyA | 2.0e-156 | 511 | 851 | 343 | + Alpha amylase catalytic domain found in archaeal, bacterial, and plant Alpha-amylases (also called 1,4-alpha-D-glucan-4-glucanohydrolase). AmyA (EC 3.2.1.1) catalyzes the hydrolysis of alpha-(1,4) glycosidic linkages of glycogen, starch, related polysaccharides, and some oligosaccharides. This group includes AmyA from bacteria, archaea, water fleas, and plants. The Alpha-amylase family comprises the largest family of glycoside hydrolases (GH), with the majority of enzymes acting on starch, glycogen, and related oligo- and polysaccharides. These proteins catalyze the transformation of alpha-1,4 and alpha-1,6 glucosidic linkages with retention of the anomeric center. The protein is described as having 3 domains: A, B, C. A is a (beta/alpha) 8-barrel; B is a loop between the beta 3 strand and alpha 3 helix of A; C is the C-terminal extension characterized by a Greek key. The majority of the enzymes have an active site cleft found between domains A and B where a triad of catalytic residues (Asp, Glu and Asp) performs catalysis. Other members of this family have lost the catalytic activity as in the case of the human 4F2hc, or only have 2 residues that serve as the catalytic nucleophile and the acid/base, such as Thermus A4 beta-galactosidase with 2 Glu residues (GH42) and human alpha-galactosidase with 2 Asp residues (GH31). The family members are quite extensive and include: alpha amylase, maltosyltransferase, cyclodextrin glycotransferase, maltogenic amylase, neopullulanase, isoamylase, 1,4-alpha-D-glucan maltotetrahydrolase, 4-alpha-glucotransferase, oligo-1,6-glucosidase, amylosucrase, sucrose phosphorylase, and amylomaltase. | ||
| PLN02361 | PLN02361 | 9.0e-164 | 507 | 898 | 397 | + alpha-amylase | ||
| PLN02784 | PLN02784 | 0 | 76 | 900 | 835 | + alpha-amylase | ||
| Gene Ontology | |
|---|---|
| GO Term | Description |
| GO:0003824 | catalytic activity |
| GO:0004556 | alpha-amylase activity |
| GO:0005509 | calcium ion binding |
| GO:0005975 | carbohydrate metabolic process |
| GO:0043169 | cation binding |
| Annotations - NR Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| GenBank | AAX33231.1 | 0 | 3 | 900 | 2 | 899 | plastid alpha-amylase [Malus x domestica] |
| EMBL | CBI32016.1 | 0 | 71 | 900 | 59 | 883 | unnamed protein product [Vitis vinifera] |
| RefSeq | NP_564977.1 | 0 | 45 | 900 | 35 | 885 | AMY3 (ALPHA-AMYLASE-LIKE 3); alpha-amylase [Arabidopsis thaliana] |
| RefSeq | XP_002270049.1 | 0 | 71 | 900 | 59 | 899 | PREDICTED: hypothetical protein [Vitis vinifera] |
| RefSeq | XP_002520134.1 | 0 | 3 | 900 | 2 | 898 | alpha-amylase, putative [Ricinus communis] |
| Annotations - PDB Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| PDB | 2qpu_C | 0 | 509 | 900 | 1 | 403 | A Chain A, Sugar Tongs Mutant S378p In Complex With Acarbose |
| PDB | 2qpu_B | 0 | 509 | 900 | 1 | 403 | A Chain A, Sugar Tongs Mutant S378p In Complex With Acarbose |
| PDB | 2qpu_A | 0 | 509 | 900 | 1 | 403 | A Chain A, Sugar Tongs Mutant S378p In Complex With Acarbose |
| PDB | 3bsg_A | 0 | 509 | 900 | 1 | 403 | A Chain A, Barley Alpha-Amylase Isozyme 1 (Amy1) H395a Mutant |
| PDB | 1rpk_A | 0 | 509 | 900 | 1 | 403 | A Chain A, Barley Alpha-Amylase Isozyme 1 (Amy1) H395a Mutant |
