| Basic Information | |
|---|---|
| Species | Linum usitatissimum |
| Cazyme ID | Lus10030923 |
| Family | GH16 |
| Protein Properties | Length: 295 Molecular Weight: 34151.9 Isoelectric Point: 8.8286 |
| Chromosome | Chromosome/Scaffold: 261 Start: 6006 End: 7957 |
| Description | xyloglucan endotransglucosylase/hydrolase 5 |
| View CDS | |
| External Links |
|---|
| CAZyDB |
| Signature Domain Download full data set without filtering | |||
|---|---|---|---|
| Family | Start | End | Evalue |
| GH16 | 48 | 211 | 6.9e-27 |
| YFNGGSEIQLHLDKYTGTGFQSKGSYLFGHFSMQMKLVPGDSAGTVTAFYLSSQNSEHDEIDFEFLGNRSGQPYILQTNVFTGGKGDREQRIYLWFDPTK AYHSYSVLWTSNIIAFFVDDVPIRVFKNLKNMGVKFPFEQPMKIYSSLWNADDWATRGGLEKTD | |||
| Full Sequence |
|---|
| Protein Sequence Length: 295 Download |
| MATRNMVMVL MMAMLLCGTT MALAPRKPVP VPFGRNYAPT WAFDHIKYFN GGSEIQLHLD 60 KYTGTGFQSK GSYLFGHFSM QMKLVPGDSA GTVTAFYLSS QNSEHDEIDF EFLGNRSGQP 120 YILQTNVFTG GKGDREQRIY LWFDPTKAYH SYSVLWTSNI IAFFVDDVPI RVFKNLKNMG 180 VKFPFEQPMK IYSSLWNADD WATRGGLEKT DWSKAPFIAS YREFHVDGCE ASVQAKFCAT 240 QGKRWWDQKE FQDLDAYQYR RLKWVRQRFT IYNYCTDRVR YPSLPAECRP DRDI* 300 |
| Functional Domains Download unfiltered results here | ||||||
|---|---|---|---|---|---|---|
| Cdd ID | Domain | E-Value | Start | End | Length | Domain Description |
| cd02175 | GH16_lichenase | 7.0e-27 | 39 | 202 | 176 | + lichenase, member of glycosyl hydrolase family 16. Lichenase, also known as 1,3-1,4-beta-glucanase, is a member of glycosyl hydrolase family 16, that specifically cleaves 1,4-beta-D-glucosidic bonds in mixed-linked beta glucans that also contain 1,3-beta-D-glucosidic linkages. Natural substrates of beta-glucanase are beta-glucans from grain endosperm cell walls or lichenan from the Islandic moss, Cetraria islandica. This protein is found not only in bacteria but also in anaerobic fungi. This domain includes two seven-stranded antiparallel beta-sheets that are adjacent to one another forming a compact, jellyroll beta-sandwich structure. |
| cd02183 | GH16_fungal_CRH1_transglycosylase | 4.0e-28 | 45 | 226 | 197 | + glycosylphosphatidylinositol-glucanosyltransferase. Group of fungal GH16 members related to Saccharomyces cerevisiae Crh1p. Chr1p and Crh2p are transglycosylases that are required for the linkage of chitin to beta(1-3)glucose branches of beta(1-6)glucan, an important step in the assembly of new cell wall. Both have been shown to be glycosylphosphatidylinositol (GPI)-anchored. A third homologous protein, Crr1p, functions in the formation of the spore wall. They belongs to the family 16 of glycosyl hydrolases that includes lichenase, xyloglucan endotransglycosylase (XET), beta-agarase, kappa-carrageenase, endo-beta-1,3-glucanase, endo-beta-1,3-1,4-glucanase, and endo-beta-galactosidase, all of which have a conserved jelly roll fold with a deep active site channel harboring the catalytic residues. |
| pfam00722 | Glyco_hydro_16 | 9.0e-77 | 33 | 214 | 184 | + Glycosyl hydrolases family 16. |
| PLN03161 | PLN03161 | 2.0e-99 | 26 | 288 | 267 | + Probable xyloglucan endotransglucosylase/hydrolase protein; Provisional |
| cd02176 | GH16_XET | 3.0e-162 | 28 | 288 | 263 | + Xyloglucan endotransglycosylase, member of glycosyl hydrolase family 16. Xyloglucan endotransglycosylases (XETs) cleave and religate xyloglucan polymers in plant cell walls via a transglycosylation mechanism. Xyloglucan is a soluble hemicellulose with a backbone of beta-1,4-linked glucose units, partially substituted with alpha-1,6-linked xylopyranose branches. It binds noncovalently to cellulose, cross-linking the adjacent cellulose microfibrils, giving it a key structural role as a matrix polymer. Therefore, XET plays an important role in all plant processes that require cell wall remodeling. |
| Gene Ontology | |
|---|---|
| GO Term | Description |
| GO:0004553 | hydrolase activity, hydrolyzing O-glycosyl compounds |
| GO:0005618 | cell wall |
| GO:0005975 | carbohydrate metabolic process |
| GO:0006073 | cellular glucan metabolic process |
| GO:0016762 | xyloglucan:xyloglucosyl transferase activity |
| Annotations - NR Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| GenBank | ABK92523.1 | 0 | 1 | 294 | 1 | 294 | unknown [Populus trichocarpa] |
| GenBank | ABM91069.1 | 0 | 1 | 294 | 1 | 293 | xyloglucan endotransglycosylase/hydrolase precursor XTH-27 [Populus trichocarpa] |
| RefSeq | XP_002297895.1 | 0 | 1 | 294 | 1 | 293 | predicted protein [Populus trichocarpa] |
| RefSeq | XP_002304625.1 | 0 | 1 | 294 | 1 | 294 | predicted protein [Populus trichocarpa] |
| RefSeq | XP_002512703.1 | 0 | 1 | 293 | 25 | 317 | Xyloglucan endotransglucosylase/hydrolase protein A precursor, putative [Ricinus communis] |
| Annotations - PDB Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| PDB | 1un1_B | 0 | 19 | 294 | 3 | 278 | A Chain A, Crystal Structure Of A Native Endo Beta-1,3-Glucanase (Hev B 2), A Major Allergen From Hevea Brasiliensis |
| PDB | 1un1_A | 0 | 19 | 294 | 3 | 278 | A Chain A, Crystal Structure Of A Native Endo Beta-1,3-Glucanase (Hev B 2), A Major Allergen From Hevea Brasiliensis |
| PDB | 1umz_B | 0 | 19 | 294 | 3 | 278 | A Chain A, Xyloglucan Endotransglycosylase In Complex With The Xyloglucan Nonasaccharide Xllg. |
| PDB | 1umz_A | 0 | 19 | 294 | 3 | 278 | A Chain A, Xyloglucan Endotransglycosylase In Complex With The Xyloglucan Nonasaccharide Xllg. |
| PDB | 2vh9_B | 0 | 5 | 288 | 7 | 290 | A Chain A, Crystal Structure Of Nxg1-Deltayniig In Complex With Xllg, A Xyloglucan Derived Oligosaccharide |
