Basic Information | |
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Species | Linum usitatissimum |
Cazyme ID | Lus10034315 |
Family | GH31 |
Protein Properties | Length: 938 Molecular Weight: 104198 Isoelectric Point: 6.586 |
Chromosome | Chromosome/Scaffold: 310 Start: 570507 End: 575676 |
Description | alpha-xylosidase 1 |
View CDS |
External Links |
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CAZyDB |
Signature Domain Download full data set without filtering | |||
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Family | Start | End | Evalue |
GH31 | 278 | 769 | 0 |
FYFFSGPSPLGVIDQYTSLVGRPAPMPYWSFGFHQCRWGYHNLSVVEDVVENYKKAKIPLDVIWNDDDHMDFHKDFTLSPVSYPREKLAAFLEKVHSSGM KYIVLIDPGINVNSTYGVYQRGIADDVFIKYEGQNYLAQVWPGAVYFPDFLNPKTVSWWGDEIRRFHEMVPVDGLWIDMNEASNFCTGLCTIPKGKKCPT GTGPGWNCCLDCKNITKTRWDEPPYKINASGIQSPIGYKTIATSATHYNGVLEYDAHSLYGFSQAIATHKALQNLQGKRPFILSRSTYVGSGKYTAHWTG DNQGTWENLKYSISTMLNFGIFGVPMVGADICGFYPQPTEELCNRWIEVGAFYPFSRDHANFYSPRQELYQWESVAESARNALGLRYKLLPYLYTLSYEA HNTGAPITRPLFFSFPTYTDCYGVSTQFLLGSSLMISPVLEQGKSQVKALFPPGSWYSMFDMSKAINSKGEYYTLDAPLHVVNVHLYQNTIL |
Full Sequence |
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Protein Sequence Length: 938 Download |
MVLSPSSSLL FSFLLLLLCF HGVTSKKSSS SSSSVPSKIG KGYRLISIEE TPDGGFLGHL 60 QVKQKNSVYG SDIPLLQLYV KHETDDRLRV HITDAEKQRW EVPYNLLPRE NPPVAKQTIG 120 RSRKNLIGVQ DYANSGELIF SYTADPFSFA VRRKSNGETL FNTSSDGSPF GPIVFKDQYL 180 EISTKLPADA SLYGLGENTQ ANGIKLKPGE PYTLYTLDIS AINLNTDLYG SHPVYMDLRN 240 VQGKPNAHGV LLLNSNGMDV FYKGNSLTYK VIGGVFDFYF FSGPSPLGVI DQYTSLVGRP 300 APMPYWSFGF HQCRWGYHNL SVVEDVVENY KKAKIPLDVI WNDDDHMDFH KDFTLSPVSY 360 PREKLAAFLE KVHSSGMKYI VLIDPGINVN STYGVYQRGI ADDVFIKYEG QNYLAQVWPG 420 AVYFPDFLNP KTVSWWGDEI RRFHEMVPVD GLWIDMNEAS NFCTGLCTIP KGKKCPTGTG 480 PGWNCCLDCK NITKTRWDEP PYKINASGIQ SPIGYKTIAT SATHYNGVLE YDAHSLYGFS 540 QAIATHKALQ NLQGKRPFIL SRSTYVGSGK YTAHWTGDNQ GTWENLKYSI STMLNFGIFG 600 VPMVGADICG FYPQPTEELC NRWIEVGAFY PFSRDHANFY SPRQELYQWE SVAESARNAL 660 GLRYKLLPYL YTLSYEAHNT GAPITRPLFF SFPTYTDCYG VSTQFLLGSS LMISPVLEQG 720 KSQVKALFPP GSWYSMFDMS KAINSKGEYY TLDAPLHVVN VHLYQNTILP MQRGGMISRD 780 ARMTPFTLVV TFPAGASDGE EAKGSLFIDN DELPEMKLGN GESSYVDFYA TVSKGVVKMW 840 SEVQEGKFAL SKGLTIEKVS VLGLGGSGSP SEVEVDGNAM SSKVQVSTME QIHMESPSSS 900 LNGGDGDEKA KKSMMVEVSG LEIPVGKNFA MTWHMGM* |
Functional Domains Download unfiltered results here | ||||||||
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Cdd ID | Domain | E-Value | Start | End | Length | Domain Description | ||
cd06602 | GH31_MGAM_SI_GAA | 2.0e-86 | 298 | 462 | 169 | + This family includes the following three closely related glycosyl hydrolase family 31 (GH31) enzymes: maltase-glucoamylase (MGAM), sucrase-isomaltase (SI), and lysosomal acid alpha-glucosidase (GAA), also known as acid-maltase. MGAM is one of the two enzymes responsible for catalyzing the last glucose-releasing step in starch digestion. SI is implicated in the digestion of dietary starch and major disaccharides such as sucrose and isomaltose, while GAA degrades glycogen in the lysosome, cleaving both alpha-1,4 and alpha-1,6 glucosidic linkages. MGAM and SI are anchored to small-intestinal brush-border epithelial cells. The absence of SI from the brush border membrane or its malfunction is associated with malabsorption disorders such as congenital sucrase-isomaltase deficiency (CSID). The domain architectures of MGAM and SI include two tandem GH31 catalytic domains, an N-terminal domain found near the membrane-bound end, and a C-terminal luminal domain. Both of the tandem GH31 domains of MGAM and SI are included in this family. The domain architecture of GAA includes an N-terminal TFF (trefoil factor family) domain in addition to the GH31 catalytic domain. Deficient GAA expression causes pompe disease, an autosomal recessive genetic disorder also known as glycogen storage disease type II (GSDII). | ||
cd06602 | GH31_MGAM_SI_GAA | 3.0e-93 | 531 | 694 | 165 | + This family includes the following three closely related glycosyl hydrolase family 31 (GH31) enzymes: maltase-glucoamylase (MGAM), sucrase-isomaltase (SI), and lysosomal acid alpha-glucosidase (GAA), also known as acid-maltase. MGAM is one of the two enzymes responsible for catalyzing the last glucose-releasing step in starch digestion. SI is implicated in the digestion of dietary starch and major disaccharides such as sucrose and isomaltose, while GAA degrades glycogen in the lysosome, cleaving both alpha-1,4 and alpha-1,6 glucosidic linkages. MGAM and SI are anchored to small-intestinal brush-border epithelial cells. The absence of SI from the brush border membrane or its malfunction is associated with malabsorption disorders such as congenital sucrase-isomaltase deficiency (CSID). The domain architectures of MGAM and SI include two tandem GH31 catalytic domains, an N-terminal domain found near the membrane-bound end, and a C-terminal luminal domain. Both of the tandem GH31 domains of MGAM and SI are included in this family. The domain architecture of GAA includes an N-terminal TFF (trefoil factor family) domain in addition to the GH31 catalytic domain. Deficient GAA expression causes pompe disease, an autosomal recessive genetic disorder also known as glycogen storage disease type II (GSDII). | ||
COG1501 | COG1501 | 1.0e-115 | 49 | 858 | 843 | + Alpha-glucosidases, family 31 of glycosyl hydrolases [Carbohydrate transport and metabolism] | ||
cd06604 | GH31_glucosidase_II_MalA | 4.0e-117 | 298 | 681 | 390 | + Alpha-glucosidase II (alpha-D-glucoside glucohydrolase) is a glycosyl hydrolase family 31 (GH31) enzyme, found in bacteria and plants, which has exo-alpha-1,4-glucosidase and oligo-1,6-glucosidase activities. Alpha-glucosidase II has been characterized in Bacillus thermoamyloliquefaciens where it forms a homohexamer. This family also includes the MalA alpha-glucosidase from Sulfolobus sulfataricus and the AglA alpha-glucosidase from Picrophilus torridus. MalA is part of the carbohydrate-metabolizing machinery that allows this organism to utilize carbohydrates, such as maltose, as the sole carbon and energy source. | ||
pfam01055 | Glyco_hydro_31 | 0 | 279 | 769 | 496 | + Glycosyl hydrolases family 31. Glycosyl hydrolases are key enzymes of carbohydrate metabolism. Family 31 comprises of enzymes that are, or similar to, alpha- galactosidases. |
Gene Ontology | |
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GO Term | Description |
GO:0004553 | hydrolase activity, hydrolyzing O-glycosyl compounds |
GO:0005975 | carbohydrate metabolic process |
Annotations - NR Download unfiltered results here | |||||||
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Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
GenBank | AAD05539.1 | 0 | 39 | 937 | 23 | 906 | alpha-xylosidase precursor [Arabidopsis thaliana] |
RefSeq | XP_002282429.1 | 0 | 12 | 937 | 4 | 922 | PREDICTED: hypothetical protein isoform 1 [Vitis vinifera] |
RefSeq | XP_002311455.1 | 0 | 28 | 937 | 4 | 908 | predicted protein [Populus trichocarpa] |
RefSeq | XP_002315944.1 | 0 | 10 | 937 | 1 | 926 | predicted protein [Populus trichocarpa] |
RefSeq | XP_002531635.1 | 0 | 5 | 937 | 3 | 928 | alpha-glucosidase, putative [Ricinus communis] |
Annotations - PDB Download unfiltered results here | |||||||
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Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
PDB | 3w38_A | 0 | 14 | 885 | 20 | 872 | A Chain A, E. Coli Gsp Amidase Cys59 Sulfenic Acid |
PDB | 3w37_A | 0 | 14 | 885 | 20 | 872 | A Chain A, E. Coli Gsp Amidase Cys59 Sulfenic Acid |
PDB | 3lpp_D | 0 | 67 | 933 | 104 | 898 | A Chain A, E. Coli Gsp Amidase Cys59 Sulfenic Acid |
PDB | 3lpp_C | 0 | 67 | 933 | 104 | 898 | A Chain A, E. Coli Gsp Amidase Cys59 Sulfenic Acid |
PDB | 3lpp_B | 0 | 67 | 933 | 104 | 898 | A Chain A, E. Coli Gsp Amidase Cys59 Sulfenic Acid |