| Basic Information | |
|---|---|
| Species | Linum usitatissimum |
| Cazyme ID | Lus10037211 |
| Family | CBM45 |
| Protein Properties | Length: 905 Molecular Weight: 101687 Isoelectric Point: 5.4897 |
| Chromosome | Chromosome/Scaffold: 462 Start: 781699 End: 786391 |
| Description | alpha-amylase-like 3 |
| View CDS | |
| External Links |
|---|
| CAZyDB |
| Signature Domain Download full data set without filtering | |||
|---|---|---|---|
| Family | Start | End | Evalue |
| CBM45 | 305 | 381 | 1.4e-23 |
| VHWGVCRDDEKNWEIPPGPHPPETIIFKEKALRTSLQSKEDGGGCSGLFTLDEDLAGFLFVLKLNDSTWLKCMGNDF | |||
| CBM45 | 126 | 209 | 2.8e-26 |
| LHWGVTYVDDLGSEWDQPPEHMKPYGSVPVKDYAVETPLQKASKDDPFYELKIDIDPNCAISAINFVLKDEETGTWYQYEGRDF | |||
| GH13 | 523 | 823 | 1.9e-36 |
| EKAKDIASLGFTVVWLPPPTDSVSPEGYMPRDLYNLNSRYGNIDELKDLVRTLHKSGIKVLGDAVLNHRCAQYQNQNGIWNMFGGRLNWDDRAVVADDPH FQGRGNKSSGDNFHAAPNIDHSQDFVRKDLKEWMCWLRKEIGYDGWRLDFVRGFWGGYVKDYMDATSPDFAVGEYWDSLSYSYGEMDHNQDAHRQRIIDW INATSGTAGAFDVTTKGILHATTLFTEMVYFMQALERCEYWRLSDSKGKPPGVVGWWPSRAVTFVENHDTGSTQGHWRFPGGKEMQGYAYILTHPGTPAV F | |||
| Full Sequence |
|---|
| Protein Sequence Length: 905 Download |
| MSSSVTVEPL LRCHRRMEAS GFRKLACSST SSSALNCPRQ KQFFRGASFR KCKLLRGSVV 60 IKASSTDTVV ADTTDESPVD AFFRESFPVT RTETVEGKMF VRVDQENGEG NWQLTVGCTI 120 PGNWILHWGV TYVDDLGSEW DQPPEHMKPY GSVPVKDYAV ETPLQKASKD DPFYELKIDI 180 DPNCAISAIN FVLKDEETGT WYQYEGRDFK VVLGDNRLEG GNGTSSSNFP GPFLYNPLLK 240 ATTEDGIGEH NDDKGEDQKL EGFYEEQTIT KHKAIENSVS VLVRRCPETF RNLVHLETDL 300 PAAVVHWGVC RDDEKNWEIP PGPHPPETII FKEKALRTSL QSKEDGGGCS GLFTLDEDLA 360 GFLFVLKLND STWLKCMGND FCIPLSSSSS SLSQPGQEQS KDAPASERGV GANQGSGTAF 420 TDEIIKEIRH LVSGISAESS QKVKTKEAQE SILQEIEKLA AEAYSIFRSS TPTFKEEAVL 480 EEELKKKPPP LKFSGTGSGY EILLQGFNWE SHKSGRWYME FKEKAKDIAS LGFTVVWLPP 540 PTDSVSPEGY MPRDLYNLNS RYGNIDELKD LVRTLHKSGI KVLGDAVLNH RCAQYQNQNG 600 IWNMFGGRLN WDDRAVVADD PHFQGRGNKS SGDNFHAAPN IDHSQDFVRK DLKEWMCWLR 660 KEIGYDGWRL DFVRGFWGGY VKDYMDATSP DFAVGEYWDS LSYSYGEMDH NQDAHRQRII 720 DWINATSGTA GAFDVTTKGI LHATTLFTEM VYFMQALERC EYWRLSDSKG KPPGVVGWWP 780 SRAVTFVENH DTGSTQGHWR FPGGKEMQGY AYILTHPGTP AVFFDHVFYS DYRSEIAALL 840 AFRNQQKIHC RSIVKIAKAE RDVYAAIIDD KVAVKIGPGH FEPQNGNWKS ALEGRDYKLW 900 ELSS* 960 |
| Functional Domains Download unfiltered results here | ||||||||
|---|---|---|---|---|---|---|---|---|
| Cdd ID | Domain | E-Value | Start | End | Length | Domain Description | ||
| PRK09441 | PRK09441 | 2.0e-46 | 501 | 832 | 409 | + cytoplasmic alpha-amylase; Reviewed | ||
| PLN00196 | PLN00196 | 2.0e-134 | 501 | 901 | 417 | + alpha-amylase; Provisional | ||
| cd11314 | AmyAc_arch_bac_plant_AmyA | 2.0e-161 | 502 | 854 | 355 | + Alpha amylase catalytic domain found in archaeal, bacterial, and plant Alpha-amylases (also called 1,4-alpha-D-glucan-4-glucanohydrolase). AmyA (EC 3.2.1.1) catalyzes the hydrolysis of alpha-(1,4) glycosidic linkages of glycogen, starch, related polysaccharides, and some oligosaccharides. This group includes AmyA from bacteria, archaea, water fleas, and plants. The Alpha-amylase family comprises the largest family of glycoside hydrolases (GH), with the majority of enzymes acting on starch, glycogen, and related oligo- and polysaccharides. These proteins catalyze the transformation of alpha-1,4 and alpha-1,6 glucosidic linkages with retention of the anomeric center. The protein is described as having 3 domains: A, B, C. A is a (beta/alpha) 8-barrel; B is a loop between the beta 3 strand and alpha 3 helix of A; C is the C-terminal extension characterized by a Greek key. The majority of the enzymes have an active site cleft found between domains A and B where a triad of catalytic residues (Asp, Glu and Asp) performs catalysis. Other members of this family have lost the catalytic activity as in the case of the human 4F2hc, or only have 2 residues that serve as the catalytic nucleophile and the acid/base, such as Thermus A4 beta-galactosidase with 2 Glu residues (GH42) and human alpha-galactosidase with 2 Asp residues (GH31). The family members are quite extensive and include: alpha amylase, maltosyltransferase, cyclodextrin glycotransferase, maltogenic amylase, neopullulanase, isoamylase, 1,4-alpha-D-glucan maltotetrahydrolase, 4-alpha-glucotransferase, oligo-1,6-glucosidase, amylosucrase, sucrose phosphorylase, and amylomaltase. | ||
| PLN02361 | PLN02361 | 4.0e-167 | 498 | 901 | 410 | + alpha-amylase | ||
| PLN02784 | PLN02784 | 0 | 3 | 903 | 916 | + alpha-amylase | ||
| Gene Ontology | |
|---|---|
| GO Term | Description |
| GO:0003824 | catalytic activity |
| GO:0004556 | alpha-amylase activity |
| GO:0005509 | calcium ion binding |
| GO:0005975 | carbohydrate metabolic process |
| GO:0043169 | cation binding |
| Annotations - NR Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| GenBank | AAX33231.1 | 0 | 3 | 903 | 2 | 901 | plastid alpha-amylase [Malus x domestica] |
| EMBL | CAN69906.1 | 0 | 3 | 903 | 2 | 887 | hypothetical protein [Vitis vinifera] |
| EMBL | CBI32016.1 | 0 | 3 | 903 | 2 | 885 | unnamed protein product [Vitis vinifera] |
| RefSeq | XP_002270049.1 | 0 | 3 | 903 | 2 | 901 | PREDICTED: hypothetical protein [Vitis vinifera] |
| RefSeq | XP_002520134.1 | 0 | 3 | 903 | 2 | 900 | alpha-amylase, putative [Ricinus communis] |
| Annotations - PDB Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| PDB | 2qpu_C | 0 | 500 | 901 | 1 | 403 | A Chain A, Sugar Tongs Mutant S378p In Complex With Acarbose |
| PDB | 2qpu_B | 0 | 500 | 901 | 1 | 403 | A Chain A, Sugar Tongs Mutant S378p In Complex With Acarbose |
| PDB | 2qpu_A | 0 | 500 | 901 | 1 | 403 | A Chain A, Sugar Tongs Mutant S378p In Complex With Acarbose |
| PDB | 3bsg_A | 0 | 500 | 901 | 1 | 403 | A Chain A, Barley Alpha-Amylase Isozyme 1 (Amy1) H395a Mutant |
| PDB | 2qps_A | 0 | 500 | 901 | 1 | 403 | A Chain A, "sugar Tongs" Mutant Y380a In Complex With Acarb |
| Hydropathy |
|---|
 |
| EST Download unfiltered results here | ||||
|---|---|---|---|---|
| Hit | Length | Start | End | EValue |
| EG631183 | 922 | 3 | 903 | 0 |
| HO826981 | 412 | 494 | 903 | 0 |
| DR932783 | 300 | 501 | 800 | 0 |
| HO811991 | 311 | 595 | 903 | 0 |
| HO826981 | 20 | 455 | 474 | 0.48 |
| Sequence Alignments (This image is cropped. Click for full image.) |
|---|
 |
