y
Basic Information | |
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Species | Picea abies |
Cazyme ID | MA_102800g0010 |
Family | GH31 |
Protein Properties | Length: 867 Molecular Weight: 97537.1 Isoelectric Point: 4.9901 |
View CDS |
Signature Domain Download full data set without filtering | |||
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Family | Start | End | Evalue |
GH31 | 210 | 702 | 0 |
FYFFSGPSPLAVVQQYTELIGRPAPMPYWSFGFHQCRYGYENISVVESVVDGYAAAKIPLETIWTDIDYMDNFKDFTLDPVNFPEMEMQKFVERIHTNGQ KYVLIIDPGISLNETYKTFQRGIQDDIFVKRGGEPYLAQVWPGPVYFPDFLNPNTQSFWTKEISEFHETVAFDGLWLDMNEVSNFCSGITCSLPNEGDCP RKDAQTDCCLFCSNDNATKWDDPPYKINASGIQRPLGIKTMAASAVHYDDILEYNAHNLYGLSEAIMTNTALKSALKKRPFILTRSTFLGSGVHAAHWTG DNAATWDDLWYSIPTMLSFGLFGVPMVGADICGFSHDTTEELCNRWIQLGSFYPFARDHSEKGSKRQELYLWDSVTRSAKKALALRYKLLPYIYTLSFEA HTNGSPIARPLFFTFPEDPNTLGISTQFLLGKGVLVSPVLTQGAVTVDAYFPKGTWYNLFNVSDSITVENGSYVTLEAPLDTINVHVYEGTIL |
Full Sequence |
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Protein Sequence Length: 867 Download |
MSHLPSTSTR TVGSSSCWYE SNDRLRVYIT DAEKQRWEVP RDILPRYDGV NNKSTHTDKD 60 EGDSKDRLHK LLFTYSNNPF GFAITRRSSG EILFNSTAIY NNGNLFGNMV FKDQYLEIST 120 SLPLAASLYG LGESTRPGFR LNRNESYTLW NLDIGSVNLY LDLYGSHPFY MDVRAGGVAH 180 GVLLLNSNGM DVQYGGSYLT YRTIGGVLDF YFFSGPSPLA VVQQYTELIG RPAPMPYWSF 240 GFHQCRYGYE NISVVESVVD GYAAAKIPLE TIWTDIDYMD NFKDFTLDPV NFPEMEMQKF 300 VERIHTNGQK YVLIIDPGIS LNETYKTFQR GIQDDIFVKR GGEPYLAQVW PGPVYFPDFL 360 NPNTQSFWTK EISEFHETVA FDGLWLDMNE VSNFCSGITC SLPNEGDCPR KDAQTDCCLF 420 CSNDNATKWD DPPYKINASG IQRPLGIKTM AASAVHYDDI LEYNAHNLYG LSEAIMTNTA 480 LKSALKKRPF ILTRSTFLGS GVHAAHWTGD NAATWDDLWY SIPTMLSFGL FGVPMVGADI 540 CGFSHDTTEE LCNRWIQLGS FYPFARDHSE KGSKRQELYL WDSVTRSAKK ALALRYKLLP 600 YIYTLSFEAH TNGSPIARPL FFTFPEDPNT LGISTQFLLG KGVLVSPVLT QGAVTVDAYF 660 PKGTWYNLFN VSDSITVENG SYVTLEAPLD TINVHVYEGT ILPMQEMGMT TREARRSPFS 720 LVVALGGRKE AYGDLYVDNG EDLEMVIEHG KSSYIEFYAN IVKGGVKLRS EVQMGEYAIR 780 EGWLLENITV VGAISAADSH YANSYTKLES IKDKTTLDVS ASSSMKRIIT DFKPEMDHYN 840 SLDVLLVISN LSLPIGKNIE LVIPWEK 900 |
Functional Domains Download unfiltered results here | ||||||||
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Cdd ID | Domain | E-Value | Start | End | Length | Domain Description | ||
pfam01055 | Glyco_hydro_31 | 9.0e-83 | 211 | 399 | 189 | + Glycosyl hydrolases family 31. Glycosyl hydrolases are key enzymes of carbohydrate metabolism. Family 31 comprises of enzymes that are, or similar to, alpha- galactosidases. | ||
cd06602 | GH31_MGAM_SI_GAA | 1.0e-90 | 463 | 631 | 170 | + This family includes the following three closely related glycosyl hydrolase family 31 (GH31) enzymes: maltase-glucoamylase (MGAM), sucrase-isomaltase (SI), and lysosomal acid alpha-glucosidase (GAA), also known as acid-maltase. MGAM is one of the two enzymes responsible for catalyzing the last glucose-releasing step in starch digestion. SI is implicated in the digestion of dietary starch and major disaccharides such as sucrose and isomaltose, while GAA degrades glycogen in the lysosome, cleaving both alpha-1,4 and alpha-1,6 glucosidic linkages. MGAM and SI are anchored to small-intestinal brush-border epithelial cells. The absence of SI from the brush border membrane or its malfunction is associated with malabsorption disorders such as congenital sucrase-isomaltase deficiency (CSID). The domain architectures of MGAM and SI include two tandem GH31 catalytic domains, an N-terminal domain found near the membrane-bound end, and a C-terminal luminal domain. Both of the tandem GH31 domains of MGAM and SI are included in this family. The domain architecture of GAA includes an N-terminal TFF (trefoil factor family) domain in addition to the GH31 catalytic domain. Deficient GAA expression causes pompe disease, an autosomal recessive genetic disorder also known as glycogen storage disease type II (GSDII). | ||
cd06602 | GH31_MGAM_SI_GAA | 5.0e-93 | 230 | 394 | 169 | + This family includes the following three closely related glycosyl hydrolase family 31 (GH31) enzymes: maltase-glucoamylase (MGAM), sucrase-isomaltase (SI), and lysosomal acid alpha-glucosidase (GAA), also known as acid-maltase. MGAM is one of the two enzymes responsible for catalyzing the last glucose-releasing step in starch digestion. SI is implicated in the digestion of dietary starch and major disaccharides such as sucrose and isomaltose, while GAA degrades glycogen in the lysosome, cleaving both alpha-1,4 and alpha-1,6 glucosidic linkages. MGAM and SI are anchored to small-intestinal brush-border epithelial cells. The absence of SI from the brush border membrane or its malfunction is associated with malabsorption disorders such as congenital sucrase-isomaltase deficiency (CSID). The domain architectures of MGAM and SI include two tandem GH31 catalytic domains, an N-terminal domain found near the membrane-bound end, and a C-terminal luminal domain. Both of the tandem GH31 domains of MGAM and SI are included in this family. The domain architecture of GAA includes an N-terminal TFF (trefoil factor family) domain in addition to the GH31 catalytic domain. Deficient GAA expression causes pompe disease, an autosomal recessive genetic disorder also known as glycogen storage disease type II (GSDII). | ||
pfam01055 | Glyco_hydro_31 | 2.0e-102 | 455 | 702 | 252 | + Glycosyl hydrolases family 31. Glycosyl hydrolases are key enzymes of carbohydrate metabolism. Family 31 comprises of enzymes that are, or similar to, alpha- galactosidases. | ||
cd06604 | GH31_glucosidase_II_MalA | 5.0e-110 | 230 | 613 | 390 | + Alpha-glucosidase II (alpha-D-glucoside glucohydrolase) is a glycosyl hydrolase family 31 (GH31) enzyme, found in bacteria and plants, which has exo-alpha-1,4-glucosidase and oligo-1,6-glucosidase activities. Alpha-glucosidase II has been characterized in Bacillus thermoamyloliquefaciens where it forms a homohexamer. This family also includes the MalA alpha-glucosidase from Sulfolobus sulfataricus and the AglA alpha-glucosidase from Picrophilus torridus. MalA is part of the carbohydrate-metabolizing machinery that allows this organism to utilize carbohydrates, such as maltose, as the sole carbon and energy source. |
Annotations - NR Download unfiltered results here | |||||||
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Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
GenBank | AAL40352.1 | 0 | 19 | 861 | 71 | 906 | AF448201_1 putative alpha-xylosidase [Pinus pinaster] |
GenBank | ABR16166.1 | 0 | 19 | 861 | 68 | 902 | unknown [Picea sitchensis] |
RefSeq | XP_002282429.1 | 0 | 19 | 861 | 72 | 916 | PREDICTED: hypothetical protein isoform 1 [Vitis vinifera] |
RefSeq | XP_002311455.1 | 0 | 19 | 861 | 58 | 902 | predicted protein [Populus trichocarpa] |
RefSeq | XP_002531635.1 | 0 | 19 | 861 | 78 | 922 | alpha-glucosidase, putative [Ricinus communis] |
Annotations - PDB Download unfiltered results here | |||||||
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Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
PDB | 3w38_A | 0 | 14 | 792 | 81 | 850 | A Chain A, Crystal Structure Of Medicago Truncatula Ugt71g1 |
PDB | 3w37_A | 0 | 14 | 792 | 81 | 850 | A Chain A, Crystal Structure Of Medicago Truncatula Ugt71g1 |
PDB | 3l4z_A | 0 | 19 | 831 | 91 | 855 | A Chain A, Crystal Structure Of Medicago Truncatula Ugt71g1 |
PDB | 3l4y_A | 0 | 19 | 831 | 91 | 855 | A Chain A, Crystal Structure Of Medicago Truncatula Ugt71g1 |
PDB | 3l4x_A | 0 | 19 | 831 | 91 | 855 | A Chain A, Crystal Structure Of Medicago Truncatula Ugt71g1 |
EST Download unfiltered results here | ||||
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Hit | Length | Start | End | EValue |
HO795307 | 678 | 194 | 861 | 0 |
HO777477 | 486 | 19 | 491 | 0 |
EX429586 | 268 | 433 | 700 | 0 |
HO777477 | 20 | 503 | 522 | 2.8 |
HO777477 | 16 | 486 | 501 | 2.8 |
Sequence Alignments (This image is cropped. Click for full image.) |
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