| Basic Information | |
|---|---|
| Species | Picea abies |
| Cazyme ID | MA_10426147g0010 |
| Family | GH32 |
| Protein Properties | Length: 583 Molecular Weight: 65942 Isoelectric Point: 9.3519 |
| View CDS | |
| Signature Domain Download full data set without filtering | |||
|---|---|---|---|
| Family | Start | End | Evalue |
| GH32 | 56 | 377 | 0 |
| HFQPVKHWMNDPNAPLFYKGYYHLFYQYNPKSAFWGRIIWGHAVSRDLINWVHMAGAAIYPDEWYDVNGAWSGSATFLHKGSAPMILYTGSNNVSEQVQA MAVPKDPSDPLLREWKKIPQNPIMLPTGINASSFRDPTTAWLGSDQIWRVLVGSKRDKNKEGMALMFRSKDFVKWVKAKRPLHSARHTGMWECPDFYPVS LYGSNGLDTSTTGSSVKHVLKNSLDDSKVDYYTVGHYLPELDRYVPDNGSVEAPNGLRYDYGKFYASKTFFDDNKSRRILWGWINESDSMVDDFKKGWAS VQAIPRVVRLDPITRRSLVQWP | |||
| Full Sequence |
|---|
| Protein Sequence Length: 583 Download |
| MAIGKSCSLL CPFFFVLLIC VSQASHHVWD EASLSALNAS KSSSLAPREA YRTAYHFQPV 60 KHWMNDPNAP LFYKGYYHLF YQYNPKSAFW GRIIWGHAVS RDLINWVHMA GAAIYPDEWY 120 DVNGAWSGSA TFLHKGSAPM ILYTGSNNVS EQVQAMAVPK DPSDPLLREW KKIPQNPIML 180 PTGINASSFR DPTTAWLGSD QIWRVLVGSK RDKNKEGMAL MFRSKDFVKW VKAKRPLHSA 240 RHTGMWECPD FYPVSLYGSN GLDTSTTGSS VKHVLKNSLD DSKVDYYTVG HYLPELDRYV 300 PDNGSVEAPN GLRYDYGKFY ASKTFFDDNK SRRILWGWIN ESDSMVDDFK KGWASVQAIP 360 RVVRLDPITR RSLVQWPVPE LESLRAYNVK KDNVVLERKS VMKVEGFKSG AAQVDVEVEF 420 ELGGHHEKVE ELEGMTAQSL CSRSKTRGFG LMVLASDDLK ERSAVFFKVF KGRVNGNVER 480 KVALCVDQSR STLQSDVDKT GYGGFVSVKP NKRSLSLIVL VDHSIVESFA EGGRTCITWR 540 SYPSVAVNEN AHLFVFNYNN SPLVLRRLSA WHMNTAKQVY ESK |
| Functional Domains Download unfiltered results here | ||||||
|---|---|---|---|---|---|---|
| Cdd ID | Domain | E-Value | Start | End | Length | Domain Description |
| TIGR01322 | scrB_fam | 3.0e-55 | 51 | 544 | 511 | + sucrose-6-phosphate hydrolase. [Energy metabolism, Biosynthesis and degradation of polysaccharides]. |
| COG1621 | SacC | 8.0e-69 | 48 | 549 | 516 | + Beta-fructosidases (levanase/invertase) [Carbohydrate transport and metabolism] |
| cd08996 | GH32_B_Fructosidase | 2.0e-88 | 62 | 380 | 331 | + Glycosyl hydrolase family 32, beta-fructosidases. Glycosyl hydrolase family GH32 cleaves sucrose into fructose and glucose via beta-fructofuranosidase activity, producing invert sugar that is a mixture of dextrorotatory D-glucose and levorotatory D-fructose, thus named invertase (EC 3.2.1.26). This family also contains other fructofuranosidases such as inulinase (EC 3.2.1.7), exo-inulinase (EC 3.2.1.80), levanase (EC 3.2.1.65), and transfructosidases such sucrose:sucrose 1-fructosyltransferase (EC 2.4.1.99), fructan:fructan 1-fructosyltransferase (EC 2.4.1.100), sucrose:fructan 6-fructosyltransferase (EC 2.4.1.10), fructan:fructan 6G-fructosyltransferase (EC 2.4.1.243) and levan fructosyltransferases (EC 2.4.1.-). These retaining enzymes (i.e. they retain the configuration at anomeric carbon atom of the substrate) catalyze hydrolysis in two steps involving a covalent glycosyl enzyme intermediate: an aspartate located close to the N-terminus acts as the catalytic nucleophile and a glutamate acts as the general acid/base; a conserved aspartate residue in the Arg-Asp-Pro (RDP) motif stabilizes the transition state. These enzymes are predicted to display a 5-fold beta-propeller fold as found for GH43 and CH68. The breakdown of sucrose is widely used as a carbon or energy source by bacteria, fungi, and plants. Invertase is used commercially in the confectionery industry, since fructose has a sweeter taste than sucrose and a lower tendency to crystallize. A common structural feature of all these enzymes is a 5-bladed beta-propeller domain, similar to GH43, that contains the catalytic acid and catalytic base. A long V-shaped groove, partially enclosed at one end, forms a single extended substrate-binding surface across the face of the propeller. |
| pfam00251 | Glyco_hydro_32N | 2.0e-135 | 56 | 377 | 331 | + Glycosyl hydrolases family 32 N-terminal domain. This domain corresponds to the N-terminal domain of glycosyl hydrolase family 32 which forms a five bladed beta propeller structure. |
| smart00640 | Glyco_32 | 6.0e-168 | 56 | 533 | 486 | + Glycosyl hydrolases family 32. |
| Annotations - NR Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| GenBank | AAD10960.1 | 0 | 23 | 577 | 23 | 575 | cell wall invertase precursor [Fragaria x ananassa] |
| GenBank | AAT09980.1 | 0 | 43 | 577 | 41 | 574 | cell wall apoplastic invertase [Vitis vinifera] |
| GenBank | ABI17893.1 | 0 | 9 | 576 | 8 | 570 | cell-wall invertase [Coffea canephora] |
| RefSeq | NP_566464.1 | 0 | 23 | 577 | 27 | 582 | ATBFRUCT1; beta-fructofuranosidase/ hydrolase, hydrolyzing O-glycosyl compounds [Arabidopsis thaliana] |
| RefSeq | XP_002279788.1 | 0 | 23 | 577 | 25 | 573 | PREDICTED: hypothetical protein [Vitis vinifera] |
| Annotations - PDB Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| PDB | 2xqr_K | 0 | 49 | 577 | 2 | 535 | B Chain B, Crystal Structure Of Plant Cell Wall Invertase In Complex With A Specific Protein Inhibitor |
| PDB | 2xqr_I | 0 | 49 | 577 | 2 | 535 | B Chain B, Crystal Structure Of Plant Cell Wall Invertase In Complex With A Specific Protein Inhibitor |
| PDB | 2xqr_G | 0 | 49 | 577 | 2 | 535 | B Chain B, Crystal Structure Of Plant Cell Wall Invertase In Complex With A Specific Protein Inhibitor |
| PDB | 2xqr_E | 0 | 49 | 577 | 2 | 535 | B Chain B, Crystal Structure Of Plant Cell Wall Invertase In Complex With A Specific Protein Inhibitor |
| PDB | 2xqr_C | 0 | 49 | 577 | 2 | 535 | B Chain B, Crystal Structure Of Plant Cell Wall Invertase In Complex With A Specific Protein Inhibitor |
