Basic Information | |
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Species | Picea abies |
Cazyme ID | MA_10437270g0020 |
Family | GT8 |
Protein Properties | Length: 770 Molecular Weight: 85499.9 Isoelectric Point: 8.2642 |
View CDS |
Signature Domain Download full data set without filtering | |||
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Family | Start | End | Evalue |
GT8 | 54 | 135 | 4.3e-21 |
NQKRPKRFWGVYTKLKIFNMTNYKKVVYLDADTVVLRSIDDLFQCRKFCANLKHSERLNSGVMVVEPSESLFKDMMAKVTTL |
Full Sequence |
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Protein Sequence Length: 770 Download |
MPDEAGASAW PLLPPADEIG KTKEVIFLMG LGLGEPRKAD GWIVEHIGLL ANPNQKRPKR 60 FWGVYTKLKI FNMTNYKKVV YLDADTVVLR SIDDLFQCRK FCANLKHSER LNSGVMVVEP 120 SESLFKDMMA KVTTLPSYTG GRGVAKRAEL STEGDSWHRK LPQQLRGTRV GLSVGLLHKK 180 RSTSSRLRRR CRQPPTVATT TVRRKCFAGK VVAAEDLADE DLHVGSGVDT MHALKGVGWV 240 RFQLESSGSL EVAGVMYVLG LKLLSVSTLE DMGYAVMFED GHVLIHSEGA DTQDAIVRLG 300 IREGDQGFLN SYYGDFPNAR LFEPNLSADE LKSRPEPSME RLSTLYNADV GLYMLANKVF 360 VAFLEWLLQQ KVLKHRLVFI EFVFLGRYLD DLYNPICLVL EVTHHFGKNT VKTITMDEIE 420 GPVPGQKVLN MRSPIAISVG RATLGRIMRV IGEAVDKRVN CILITSCQFI IIIQDSRHQC 480 SSSETTIAWR GSWGIGGQLG YRILLISGRS LSKIPDINVP QVKPLSPGEV LGCTVPSSLP 540 HGTLDTAVFV SGGRFPLEAF MLANPQVQAF RYDPYLKVSV LQKYDHKGMK DMLKKVILQA 600 QRAKQWGIIL GTLGRQGNPK ILNHIEQRLA EKGILFTVFL MPEISPSKIA LFGDFVDVWV 660 QIVCPCLSIN WGEAFTKPLV TQFEAEVALG FVHPWWNRET EQEDACRHMV EGQVQSCGSC 720 AETRSSVVTC NKDSCCAGRS SVNGTASVTD IDRTKSEESC LSDGILLESF |
Functional Domains Download unfiltered results here | ||||||||
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Cdd ID | Domain | E-Value | Start | End | Length | Domain Description | ||
cd02537 | GT8_Glycogenin | 8.0e-34 | 38 | 141 | 110 | + Glycogenin belongs the GT 8 family and initiates the biosynthesis of glycogen. Glycogenin initiates the biosynthesis of glycogen by incorporating glucose residues through a self-glucosylation reaction at a Tyr residue, and then acts as substrate for chain elongation by glycogen synthase and branching enzyme. It contains a conserved DxD motif and an N-terminal beta-alpha-beta Rossmann-like fold that are common to the nucleotide-binding domains of most glycosyltransferases. The DxD motif is essential for coordination of the catalytic divalent cation, most commonly Mn2+. Glycogenin can be classified as a retaining glycosyltransferase, based on the relative anomeric stereochemistry of the substrate and product in the reaction catalyzed. It is placed in glycosyltransferase family 8 which includes lipopolysaccharide glucose and galactose transferases and galactinol synthases. | ||
COG1736 | DPH2 | 7.0e-37 | 507 | 699 | 197 | + Diphthamide synthase subunit DPH2 [Translation, ribosomal structure and biogenesis] | ||
pfam01866 | Diphthamide_syn | 1.0e-53 | 512 | 690 | 179 | + Putative diphthamide synthesis protein. Human DPH1 is a candidate tumour suppressor gene. DPH2 from yeast, which confers resistance to diphtheria toxin, has been found to be involved in diphthamide synthesis. Diphtheria toxin inhibits eukaryotic protein synthesis by ADP-ribosylating diphthamide, a posttranslationally modified histidine residue present in EF2. The exact function of the members of this family is unknown. | ||
TIGR00322 | diphth2_R | 9.0e-54 | 514 | 689 | 178 | + diphthamide biosynthesis enzyme Dph1/Dph2 domain. Archaea and Eukaryotes, but not Eubacteria, share the property of having a covalently modified residue, 2'-[3-carboxamido-3-(trimethylammonio)propyl]histidine, as a part of a cytosolic protein. The modified His, termed diphthamide, is part of translation elongation factor EF-2 and is the site for ADP-ribosylation by diphtheria toxin. This model includes both Dph1 and Dph2 from Saccharomyces cerevisiae, although only Dph2 is found in the Archaea (see TIGR03682). Dph2 has been shown to act analogously to the radical SAM (rSAM) family (pfam04055), with 4Fe-4S-assisted cleavage of S-adenosylmethionine to create a free radical, but a different organic radical than in rSAM. |
Annotations - NR Download unfiltered results here | |||||||
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Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
DDBJ | BAB10164.1 | 0 | 516 | 734 | 207 | 418 | unnamed protein product [Arabidopsis thaliana] |
RefSeq | NP_201009.1 | 0 | 516 | 734 | 192 | 403 | diphthamide synthesis DPH2 family protein [Arabidopsis thaliana] |
RefSeq | XP_001764133.1 | 0 | 514 | 718 | 192 | 398 | predicted protein [Physcomitrella patens subsp. patens] |
RefSeq | XP_002265458.1 | 0 | 519 | 706 | 206 | 396 | PREDICTED: hypothetical protein [Vitis vinifera] |
RefSeq | XP_002529006.1 | 0 | 519 | 712 | 193 | 393 | Diphthamide biosynthesis protein, putative [Ricinus communis] |
Annotations - PDB Download unfiltered results here | |||||||
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Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
PDB | 4b2q_d | 0.00000000000004 | 398 | 458 | 41 | 101 | V Chain V, Model Of The Yeast F1fo-Atp Synthase Dimer Based On Subtomogram Average |
PDB | 4b2q_D | 0.00000000000004 | 398 | 458 | 41 | 101 | V Chain V, Model Of The Yeast F1fo-Atp Synthase Dimer Based On Subtomogram Average |
PDB | 3zry_F | 0.00000000000004 | 398 | 458 | 46 | 106 | V Chain V, Model Of The Yeast F1fo-Atp Synthase Dimer Based On Subtomogram Average |
PDB | 3zry_E | 0.00000000000004 | 398 | 458 | 46 | 106 | V Chain V, Model Of The Yeast F1fo-Atp Synthase Dimer Based On Subtomogram Average |
PDB | 3zry_D | 0.00000000000004 | 398 | 458 | 46 | 106 | V Chain V, Model Of The Yeast F1fo-Atp Synthase Dimer Based On Subtomogram Average |
EST Download unfiltered results here | ||||
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Hit | Length | Start | End | EValue |
EX314344 | 213 | 553 | 765 | 0 |
GW770465 | 220 | 546 | 765 | 0 |
DR547045 | 186 | 580 | 765 | 0 |
GW770820 | 186 | 580 | 765 | 0 |
CT580900 | 186 | 580 | 765 | 0 |
Sequence Alignments (This image is cropped. Click for full image.) |
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