| Basic Information | |
|---|---|
| Species | Picea abies |
| Cazyme ID | MA_35999g0010 |
| Family | GH32 |
| Protein Properties | Length: 633 Molecular Weight: 70142.8 Isoelectric Point: 4.6069 |
| View CDS | |
| Signature Domain Download full data set without filtering | |||
|---|---|---|---|
| Family | Start | End | Evalue |
| GH32 | 102 | 419 | 0 |
| HFQPEKNWMNDPDGPLVYKGWYHLFYQYNPDSAIWGNITWGHAVSKDLVHWLYLDLAMVPDHWYDINGVWTGSATILANGSIVMLYTGSTNESVQVQNLA FPENASDPLLRKWVKSEANPVLVPPKGIELKDFRDPTTGWIGEDGAWRITIGSKLEKTGVALVFKTEDFVKYEEEDILHSVNDTGMWECVDFYPVAASGV SGLDTSANGAGVKHVLKASFDDDKHDYYAIGTYNATSQKFIPDDPELDVGLGLRYDYGKYYASKSFYDPVKERRILWGWVGETDSEQDDLRKGWASLQTI PRHIWFDNKTKTNIIQWP | |||
| Full Sequence |
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| Protein Sequence Length: 633 Download |
| MAKDLEEVAG EYVGLSDNVN KKNGIYRPAI LCVFLIAVVG LCGKTIFNKP NDGEKSEERP 60 AAAEYVISRG RIEGVSEKSV GASISPYPWT NAELSWQRTG FHFQPEKNWM NDPDGPLVYK 120 GWYHLFYQYN PDSAIWGNIT WGHAVSKDLV HWLYLDLAMV PDHWYDINGV WTGSATILAN 180 GSIVMLYTGS TNESVQVQNL AFPENASDPL LRKWVKSEAN PVLVPPKGIE LKDFRDPTTG 240 WIGEDGAWRI TIGSKLEKTG VALVFKTEDF VKYEEEDILH SVNDTGMWEC VDFYPVAASG 300 VSGLDTSANG AGVKHVLKAS FDDDKHDYYA IGTYNATSQK FIPDDPELDV GLGLRYDYGK 360 YYASKSFYDP VKERRILWGW VGETDSEQDD LRKGWASLQT IPRHIWFDNK TKTNIIQWPV 420 EEIDELRSAA LVVENVLLQP GSVVEVNTTA ASQLDIELVV EFPEDAAAID AAEGNAVYNC 480 STSGGAAGRG VLGPFGLLVI AEKNLTEHTA VYFYVAKSAD GKLQTFFCHD ESRSSLATDL 540 ILGIHGSSVP VLANETISVR TLVDHSIVES YAQGGRRCIT SRVYPTQALD GAAKLFLFNN 600 ATTAVKVQSL NVWQMETVLL HPYQQLEQEH PVD 660 |
| Functional Domains Download unfiltered results here | ||||||||
|---|---|---|---|---|---|---|---|---|
| Cdd ID | Domain | E-Value | Start | End | Length | Domain Description | ||
| TIGR01322 | scrB_fam | 2.0e-51 | 91 | 585 | 513 | + sucrose-6-phosphate hydrolase. [Energy metabolism, Biosynthesis and degradation of polysaccharides]. | ||
| COG1621 | SacC | 7.0e-65 | 98 | 617 | 536 | + Beta-fructosidases (levanase/invertase) [Carbohydrate transport and metabolism] | ||
| cd08996 | GH32_B_Fructosidase | 3.0e-93 | 108 | 422 | 322 | + Glycosyl hydrolase family 32, beta-fructosidases. Glycosyl hydrolase family GH32 cleaves sucrose into fructose and glucose via beta-fructofuranosidase activity, producing invert sugar that is a mixture of dextrorotatory D-glucose and levorotatory D-fructose, thus named invertase (EC 3.2.1.26). This family also contains other fructofuranosidases such as inulinase (EC 3.2.1.7), exo-inulinase (EC 3.2.1.80), levanase (EC 3.2.1.65), and transfructosidases such sucrose:sucrose 1-fructosyltransferase (EC 2.4.1.99), fructan:fructan 1-fructosyltransferase (EC 2.4.1.100), sucrose:fructan 6-fructosyltransferase (EC 2.4.1.10), fructan:fructan 6G-fructosyltransferase (EC 2.4.1.243) and levan fructosyltransferases (EC 2.4.1.-). These retaining enzymes (i.e. they retain the configuration at anomeric carbon atom of the substrate) catalyze hydrolysis in two steps involving a covalent glycosyl enzyme intermediate: an aspartate located close to the N-terminus acts as the catalytic nucleophile and a glutamate acts as the general acid/base; a conserved aspartate residue in the Arg-Asp-Pro (RDP) motif stabilizes the transition state. These enzymes are predicted to display a 5-fold beta-propeller fold as found for GH43 and CH68. The breakdown of sucrose is widely used as a carbon or energy source by bacteria, fungi, and plants. Invertase is used commercially in the confectionery industry, since fructose has a sweeter taste than sucrose and a lower tendency to crystallize. A common structural feature of all these enzymes is a 5-bladed beta-propeller domain, similar to GH43, that contains the catalytic acid and catalytic base. A long V-shaped groove, partially enclosed at one end, forms a single extended substrate-binding surface across the face of the propeller. | ||
| pfam00251 | Glyco_hydro_32N | 5.0e-148 | 102 | 419 | 327 | + Glycosyl hydrolases family 32 N-terminal domain. This domain corresponds to the N-terminal domain of glycosyl hydrolase family 32 which forms a five bladed beta propeller structure. | ||
| smart00640 | Glyco_32 | 2.0e-177 | 102 | 575 | 483 | + Glycosyl hydrolases family 32. | ||
| Annotations - NR Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| EMBL | CAA53098.1 | 0 | 48 | 623 | 77 | 656 | beta-fructofuranosidase [Daucus carota] |
| EMBL | CAA53099.1 | 0 | 48 | 623 | 77 | 656 | beta-fructofuranosidase [Daucus carota] |
| EMBL | CAC83577.2 | 0 | 67 | 626 | 77 | 643 | vacuolar invertase [Nicotiana tabacum] |
| Swiss-Prot | P80065 | 0 | 48 | 623 | 77 | 656 | INVB_DAUCA RecName: Full=Beta-fructofuranosidase, soluble isoenzyme I; AltName: Full=Sucrose hydrolase; AltName: Full=Invertase; AltName: Full=Saccharase; Flags: Precursor |
| RefSeq | XP_002303519.1 | 0 | 68 | 628 | 98 | 662 | predicted protein [Populus trichocarpa] |
| Annotations - PDB Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| PDB | 3ugh_B | 0 | 86 | 625 | 7 | 546 | C Chain C, The Mouse Von Willebrand Factor A1-Botrocetin Complex |
| PDB | 3ugh_A | 0 | 86 | 625 | 7 | 546 | C Chain C, The Mouse Von Willebrand Factor A1-Botrocetin Complex |
| PDB | 3ugg_B | 0 | 86 | 625 | 7 | 546 | C Chain C, The Mouse Von Willebrand Factor A1-Botrocetin Complex |
| PDB | 3ugg_A | 0 | 86 | 625 | 7 | 546 | C Chain C, The Mouse Von Willebrand Factor A1-Botrocetin Complex |
| PDB | 3ugf_B | 0 | 86 | 625 | 7 | 546 | A Chain A, Crystal Structure Of A 6-Sst6-Sft From Pachysandra Terminalis |
| Hydropathy |
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| EST Download unfiltered results here | ||||
|---|---|---|---|---|
| Hit | Length | Start | End | EValue |
| EX417157 | 274 | 360 | 633 | 0 |
| CO488687 | 301 | 314 | 613 | 0 |
| DR097982 | 268 | 234 | 501 | 0 |
| DR094404 | 263 | 360 | 622 | 0 |
| GT267661 | 259 | 339 | 597 | 0 |
| Sequence Alignments (This image is cropped. Click for full image.) |
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