| Basic Information | |
|---|---|
| Species | Malus domestica |
| Cazyme ID | MDP0000124776 |
| Family | GH32 |
| Protein Properties | Length: 678 Molecular Weight: 75834 Isoelectric Point: 6.6595 |
| Chromosome | Chromosome/Scaffold: 009378171 Start: 1408 End: 6181 |
| Description | Glycosyl hydrolases family 32 protein |
| View CDS | |
| External Links |
|---|
| NCBI Taxonomy |
| CAZyDB |
| Signature Domain Download full data set without filtering | |||
|---|---|---|---|
| Family | Start | End | Evalue |
| GH32 | 128 | 443 | 0 |
| HFQPEKNWMNGPLFYKGWYHFFYQWNPNGAIWGDIVWGHAVSKDLIHWLHLPLAMVADQWYDINGVWTGSATILPDGKIVMLYTGSTNESVQVQNLAYPA DHNDPLLTKWVKYSGNPILVPPPGIGYKDFRDPTTAWYTSQGKWRITIGSKLNKTGISLVYDTKDFKTYEQLNGVLHAVPGTGMWECVDFYPVSKTSDKG LDTSVNGPDVKHVVKASLDDDRNDYYSLGTYEEKTGKWVPDNQKIDVGIGIRYDYGIFYASKTFYDQNKERRVLWGWIGESDSENADMQKGWASVQGIPR TVVFDKKTGSNLLQWP | |||
| Full Sequence |
|---|
| Protein Sequence Length: 678 Download |
| MADPRPFLPS DPDALPYTYT PLPNPLEASE IRKRQFKELL AVFSGLLMLS LLVVIISSGN 60 NSDSHANKNE PVFLAKEPYT TRTVKLRPVP RGIAAGVSEK SNRLADAVDG PAFPWNNSML 120 SWQRTAFHFQ PEKNWMNGPL FYKGWYHFFY QWNPNGAIWG DIVWGHAVSK DLIHWLHLPL 180 AMVADQWYDI NGVWTGSATI LPDGKIVMLY TGSTNESVQV QNLAYPADHN DPLLTKWVKY 240 SGNPILVPPP GIGYKDFRDP TTAWYTSQGK WRITIGSKLN KTGISLVYDT KDFKTYEQLN 300 GVLHAVPGTG MWECVDFYPV SKTSDKGLDT SVNGPDVKHV VKASLDDDRN DYYSLGTYEE 360 KTGKWVPDNQ KIDVGIGIRY DYGIFYASKT FYDQNKERRV LWGWIGESDS ENADMQKGWA 420 SVQGIPRTVV FDKKTGSNLL QWPVEEVEKL RLSITDFDKV EVKAGSVLPL QVGTATQLDI 480 VAEFELDKKV LESVAESNEV FSCNTSAGAS HRGALGPFGL LVLADETLSE QTPVYFYVAK 540 GPGGNFDTFF CADQTRSSVA NDVKKKVTGS YVPVLKDEKL SARILVDHSI VESFAQGGRT 600 TITSRVYPTK AIYGAARLIL FNNATETSVT ASLKVWQMNS AFIRPYHPDE TSNASLNLVT 660 YIKFIVTFFI IFTLSFLR 720 |
| Functional Domains Download unfiltered results here | ||||||||
|---|---|---|---|---|---|---|---|---|
| Cdd ID | Domain | E-Value | Start | End | Length | Domain Description | ||
| TIGR01322 | scrB_fam | 7.0e-48 | 124 | 608 | 507 | + sucrose-6-phosphate hydrolase. [Energy metabolism, Biosynthesis and degradation of polysaccharides]. | ||
| COG1621 | SacC | 3.0e-70 | 124 | 637 | 526 | + Beta-fructosidases (levanase/invertase) [Carbohydrate transport and metabolism] | ||
| cd08996 | GH32_B_Fructosidase | 9.0e-88 | 134 | 446 | 322 | + Glycosyl hydrolase family 32, beta-fructosidases. Glycosyl hydrolase family GH32 cleaves sucrose into fructose and glucose via beta-fructofuranosidase activity, producing invert sugar that is a mixture of dextrorotatory D-glucose and levorotatory D-fructose, thus named invertase (EC 3.2.1.26). This family also contains other fructofuranosidases such as inulinase (EC 3.2.1.7), exo-inulinase (EC 3.2.1.80), levanase (EC 3.2.1.65), and transfructosidases such sucrose:sucrose 1-fructosyltransferase (EC 2.4.1.99), fructan:fructan 1-fructosyltransferase (EC 2.4.1.100), sucrose:fructan 6-fructosyltransferase (EC 2.4.1.10), fructan:fructan 6G-fructosyltransferase (EC 2.4.1.243) and levan fructosyltransferases (EC 2.4.1.-). These retaining enzymes (i.e. they retain the configuration at anomeric carbon atom of the substrate) catalyze hydrolysis in two steps involving a covalent glycosyl enzyme intermediate: an aspartate located close to the N-terminus acts as the catalytic nucleophile and a glutamate acts as the general acid/base; a conserved aspartate residue in the Arg-Asp-Pro (RDP) motif stabilizes the transition state. These enzymes are predicted to display a 5-fold beta-propeller fold as found for GH43 and CH68. The breakdown of sucrose is widely used as a carbon or energy source by bacteria, fungi, and plants. Invertase is used commercially in the confectionery industry, since fructose has a sweeter taste than sucrose and a lower tendency to crystallize. A common structural feature of all these enzymes is a 5-bladed beta-propeller domain, similar to GH43, that contains the catalytic acid and catalytic base. A long V-shaped groove, partially enclosed at one end, forms a single extended substrate-binding surface across the face of the propeller. | ||
| pfam00251 | Glyco_hydro_32N | 6.0e-146 | 128 | 443 | 327 | + Glycosyl hydrolases family 32 N-terminal domain. This domain corresponds to the N-terminal domain of glycosyl hydrolase family 32 which forms a five bladed beta propeller structure. | ||
| smart00640 | Glyco_32 | 0 | 128 | 598 | 484 | + Glycosyl hydrolases family 32. | ||
| Gene Ontology | |
|---|---|
| GO Term | Description |
| GO:0004564 | beta-fructofuranosidase activity |
| GO:0004575 | sucrose alpha-glucosidase activity |
| Annotations - NR Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| DDBJ | BAF34362.1 | 0 | 85 | 661 | 81 | 659 | beta-fructofuranosidase [Citrus sinensis] |
| DDBJ | BAF35859.1 | 0 | 1 | 677 | 1 | 680 | soluble acid invertase [Pyrus pyrifolia var. culta] |
| Swiss-Prot | P29001 | 0 | 1 | 652 | 1 | 649 | INVA_PHAAU RecName: Full=Acid beta-fructofuranosidase; AltName: Full=Acid sucrose hydrolase; AltName: Full=Acid invertase; Short=AI; AltName: Full=Vacuolar invertase; Contains: RecName: Full=Acid beta-fructofuranosidase 30 kDa subunit; Contains: RecName: Full=Acid beta-fructofuranosidase 38 kDa subunit; Flags: Precursor |
| RefSeq | XP_002303519.1 | 0 | 1 | 650 | 1 | 661 | predicted protein [Populus trichocarpa] |
| RefSeq | XP_002532576.1 | 0 | 1 | 646 | 1 | 645 | Acid beta-fructofuranosidase precursor, putative [Ricinus communis] |
| Annotations - PDB Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| PDB | 3ugh_B | 0 | 113 | 647 | 8 | 545 | A Chain A, The Crystal Structure Of A Cyanogenic Beta-Glucosidase From White Clover (Trifolium Repens L.), A Family 1 Glycosyl-Hydrolase |
| PDB | 3ugh_A | 0 | 113 | 647 | 8 | 545 | A Chain A, The Crystal Structure Of A Cyanogenic Beta-Glucosidase From White Clover (Trifolium Repens L.), A Family 1 Glycosyl-Hydrolase |
| PDB | 3ugg_B | 0 | 113 | 647 | 8 | 545 | A Chain A, The Crystal Structure Of A Cyanogenic Beta-Glucosidase From White Clover (Trifolium Repens L.), A Family 1 Glycosyl-Hydrolase |
| PDB | 3ugg_A | 0 | 113 | 647 | 8 | 545 | A Chain A, The Crystal Structure Of A Cyanogenic Beta-Glucosidase From White Clover (Trifolium Repens L.), A Family 1 Glycosyl-Hydrolase |
| PDB | 3ugf_B | 0 | 113 | 647 | 8 | 545 | A Chain A, Crystal Structure Of A 6-Sst6-Sft From Pachysandra Terminalis |
