| Basic Information | |
|---|---|
| Species | Malus domestica |
| Cazyme ID | MDP0000128432 |
| Family | GH16 |
| Protein Properties | Length: 496 Molecular Weight: 55933.1 Isoelectric Point: 4.7222 |
| Chromosome | Chromosome/Scaffold: 015114289 Start: 11382 End: 15891 |
| Description | xyloglucan endotransglucosylase/hydrolase 10 |
| View CDS | |
| External Links |
|---|
| NCBI Taxonomy |
| CAZyDB |
| Signature Domain Download full data set without filtering | |||
|---|---|---|---|
| Family | Start | End | Evalue |
| GH16 | 47 | 212 | 1.1e-21 |
| NTSADGKTMSLKLDQESGAGFASNQMFLFGQIDMQIKLVPGHSAGTVVAYYLTSDQPNRDEVDYEFLGNVEGKPYILQTNIFADGLDDREERINLWFDPT KDFHTYSILWNLHQIVFMVDWVPIRVYRNHADKGVAFPRWQPMSIKASLWNGDSWATGGGRDKIDW | |||
| Full Sequence |
|---|
| Protein Sequence Length: 496 Download |
| MTENNRIIIF IGLFLTLIST RTVDASVVST GDFNKDFFVT WSPGHVNTSA DGKTMSLKLD 60 QESGAGFASN QMFLFGQIDM QIKLVPGHSA GTVVAYYLTS DQPNRDEVDY EFLGNVEGKP 120 YILQTNIFAD GLDDREERIN LWFDPTKDFH TYSILWNLHQ IVFMVDWVPI RVYRNHADKG 180 VAFPRWQPMS IKASLWNGDS WATGGGRDKI DWSKGPFIAS FRNHTIDACV WKGNARFCRA 240 ESPTNWWNEE RFSTLSNAQR RLFKWVRKYH MIYDYCEDKE RFQGNLPKEW TIGGKSWLSA 300 SSGGWKNSKK YFNLPYKSES TLLVCKNAST KKAQPEGKLI PALPLPKSQV LGQVRDFLGV 360 ISEANERLYR DAKELMLGVA DLQTPEAVTA AESAISGCQP VIPLAVNSSG EESEDSSDDD 420 SSSKDGDDED SSDDSSSEDS FDEDSDDIKD DTSDKDDKET CFHAKLKTRE PDQDNALSED 480 VGNNRSKKRP RIVELS 540 |
| Functional Domains Download unfiltered results here | ||||||||
|---|---|---|---|---|---|---|---|---|
| Cdd ID | Domain | E-Value | Start | End | Length | Domain Description | ||
| cd00413 | Glyco_hydrolase_16 | 2.0e-18 | 40 | 212 | 194 | + glycosyl hydrolase family 16. The O-Glycosyl hydrolases are a widespread group of enzymes that hydrolyse the glycosidic bond between two or more carbohydrates, or between a carbohydrate and a non-carbohydrate moiety. A glycosyl hydrolase classification system based on sequence similarity has led to the definition of more than 95 different families inlcuding glycosyl hydrolase family 16. Family 16 includes lichenase, xyloglucan endotransglycosylase (XET), beta-agarase, kappa-carrageenase, endo-beta-1,3-glucanase, endo-beta-1,3-1,4-glucanase, and endo-beta-galactosidase, all of which have a conserved jelly roll fold with a deep active site channel harboring the catalytic residues. | ||
| cd02183 | GH16_fungal_CRH1_transglycosylase | 5.0e-26 | 30 | 226 | 211 | + glycosylphosphatidylinositol-glucanosyltransferase. Group of fungal GH16 members related to Saccharomyces cerevisiae Crh1p. Chr1p and Crh2p are transglycosylases that are required for the linkage of chitin to beta(1-3)glucose branches of beta(1-6)glucan, an important step in the assembly of new cell wall. Both have been shown to be glycosylphosphatidylinositol (GPI)-anchored. A third homologous protein, Crr1p, functions in the formation of the spore wall. They belongs to the family 16 of glycosyl hydrolases that includes lichenase, xyloglucan endotransglycosylase (XET), beta-agarase, kappa-carrageenase, endo-beta-1,3-glucanase, endo-beta-1,3-1,4-glucanase, and endo-beta-galactosidase, all of which have a conserved jelly roll fold with a deep active site channel harboring the catalytic residues. | ||
| pfam00722 | Glyco_hydro_16 | 5.0e-67 | 33 | 214 | 184 | + Glycosyl hydrolases family 16. | ||
| PLN03161 | PLN03161 | 4.0e-101 | 32 | 289 | 260 | + Probable xyloglucan endotransglucosylase/hydrolase protein; Provisional | ||
| cd02176 | GH16_XET | 2.0e-149 | 29 | 289 | 262 | + Xyloglucan endotransglycosylase, member of glycosyl hydrolase family 16. Xyloglucan endotransglycosylases (XETs) cleave and religate xyloglucan polymers in plant cell walls via a transglycosylation mechanism. Xyloglucan is a soluble hemicellulose with a backbone of beta-1,4-linked glucose units, partially substituted with alpha-1,6-linked xylopyranose branches. It binds noncovalently to cellulose, cross-linking the adjacent cellulose microfibrils, giving it a key structural role as a matrix polymer. Therefore, XET plays an important role in all plant processes that require cell wall remodeling. | ||
| Gene Ontology | |
|---|---|
| GO Term | Description |
| GO:0004553 | hydrolase activity, hydrolyzing O-glycosyl compounds |
| GO:0005618 | cell wall |
| GO:0005975 | carbohydrate metabolic process |
| GO:0006073 | cellular glucan metabolic process |
| GO:0016762 | xyloglucan:xyloglucosyl transferase activity |
| Annotations - NR Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| EMBL | CAN70891.1 | 0 | 20 | 289 | 2 | 271 | hypothetical protein [Vitis vinifera] |
| EMBL | CBI25107.1 | 0 | 1 | 289 | 1 | 289 | unnamed protein product [Vitis vinifera] |
| RefSeq | XP_002270633.1 | 0 | 1 | 289 | 1 | 289 | PREDICTED: hypothetical protein [Vitis vinifera] |
| RefSeq | XP_002313201.1 | 0 | 1 | 289 | 1 | 288 | predicted protein [Populus trichocarpa] |
| RefSeq | XP_002522076.1 | 0 | 1 | 289 | 1 | 288 | Xyloglucan endotransglucosylase/hydrolase protein A precursor, putative [Ricinus communis] |
| Annotations - PDB Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| PDB | 1un1_B | 0 | 33 | 288 | 17 | 271 | A Chain A, Crystal Structure Of A Putative Flavoprotein (Bacegg_01620) From Bacteroides Eggerthii Dsm 20697 At 1.40 A Resolution |
| PDB | 1un1_A | 0 | 33 | 288 | 17 | 271 | A Chain A, Crystal Structure Of A Putative Flavoprotein (Bacegg_01620) From Bacteroides Eggerthii Dsm 20697 At 1.40 A Resolution |
| PDB | 1umz_B | 0 | 33 | 288 | 17 | 271 | A Chain A, Xyloglucan Endotransglycosylase In Complex With The Xyloglucan Nonasaccharide Xllg. |
| PDB | 1umz_A | 0 | 33 | 288 | 17 | 271 | A Chain A, Xyloglucan Endotransglycosylase In Complex With The Xyloglucan Nonasaccharide Xllg. |
| PDB | 2uwb_B | 0 | 25 | 281 | 12 | 259 | A Chain A, Crystal Structure Of The Nasturtium Seedling Mutant Xyloglucanase Isoform Nxg1-Delta-Yniig |
