Basic Information | |
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Species | Malus domestica |
Cazyme ID | MDP0000139096 |
Family | GT20 |
Protein Properties | Length: 954 Molecular Weight: 107471 Isoelectric Point: 7.0516 |
Chromosome | Chromosome/Scaffold: 01251988 Start: 977 End: 7733 |
Description | trehalose-6-phosphate synthase |
View CDS |
External Links |
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NCBI Taxonomy |
CAZyDB |
Signature Domain Download full data set without filtering | |||
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Family | Start | End | Evalue |
GT20 | 83 | 550 | 0 |
RQRLLVVANRLPVSAIRRGEDSWSLEMSAGGLVSALLGVKEFEARWIGWAGVNVPDEIGQKALTRALAEKRCIPVFLDEEIVHQYYNGYCNNILWPLFHY LGLPQEDRLATTRSFQSQFAAYKKANQMFADVVNEHYEEGDVVWCHDYHLMFLPKCLKDYDSKMKVGWFLHTPFPSSEIHRTLPSRSELLRAVLAADLVG FHTYDYARHFVSACTRILGLEGTPEGVEDQGRLTRVAAFPIGIDSDRFIRGLEXPXVQEHIKKMKERLAGRKVMLGVDRLDMIKGIPQKLLAFEKFLEEN PTWRDKVVLLQIAVPTRTDVPEYQKLTSQVHEIVGRINGRFGTLTAVPIHHLDCSLDFHELCALYAVTDVALVTSLRDGMNLVSYEFVACQDAKRGVLIL SEFAGAAQSLGAGAILVNPWNITEVANSIAQALNMDSEQREKRHKHNFLHVTAHTAQEWAETFVSELN |
Full Sequence |
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Protein Sequence Length: 954 Download |
MPGNKYNGNS AHIPPTRIEX LLRERELRKN IKALNLNETF EPELQLREDE NSKAALVERF 60 LEGAAAARAL NEGCEKQDGR PSRQRLLVVA NRLPVSAIRR GEDSWSLEMS AGGLVSALLG 120 VKEFEARWIG WAGVNVPDEI GQKALTRALA EKRCIPVFLD EEIVHQYYNG YCNNILWPLF 180 HYLGLPQEDR LATTRSFQSQ FAAYKKANQM FADVVNEHYE EGDVVWCHDY HLMFLPKCLK 240 DYDSKMKVGW FLHTPFPSSE IHRTLPSRSE LLRAVLAADL VGFHTYDYAR HFVSACTRIL 300 GLEGTPEGVE DQGRLTRVAA FPIGIDSDRF IRGLEXPXVQ EHIKKMKERL AGRKVMLGVD 360 RLDMIKGIPQ KLLAFEKFLE ENPTWRDKVV LLQIAVPTRT DVPEYQKLTS QVHEIVGRIN 420 GRFGTLTAVP IHHLDCSLDF HELCALYAVT DVALVTSLRD GMNLVSYEFV ACQDAKRGVL 480 ILSEFAGAAQ SLGAGAILVN PWNITEVANS IAQALNMDSE QREKRHKHNF LHVTAHTAQE 540 WAETFVSELN DTVVAAQLRT RQVPPPLPNK DAIQSYLQAN NRLIILGFNA TLTESVDTXE 600 RRGDQIKEME LKLHPKLKET LSALCNDPTT TIVVLSGSER HVLDDNFGEL DMWLAAENGM 660 FLRLTKGEWM TTMPEHVNME WVNSVEHVFE YFKERTPRSH YERRQTSLVW NYKYAGTSFF 720 FQPSNQKKKL GSLNPISLGD DIDFGRLQAR DMLQHLWTGP ISNASVDVVQ GSRSVEVRAV 780 GVTKGAAIDR ILGEIVHSKS MTSPIDYVLC IGHFLGKDED VYTFFEPDLP PDPSSLPRTK 840 IPDGLKPNER RSSLKAPAIK SGSKSSQSKA QRAPLPNHEK KNANHNTTNP RWPSPEKVSW 900 NVLDLKKENY YSCAVGRTRT NARYLLQSSD DVVSFLKELX GASSASVSSS GSEI 960 |
Functional Domains Download unfiltered results here | ||||||||
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Cdd ID | Domain | E-Value | Start | End | Length | Domain Description | ||
PLN03064 | PLN03064 | 0 | 1 | 939 | 952 | + alpha,alpha-trehalose-phosphate synthase (UDP-forming); Provisional | ||
PLN03063 | PLN03063 | 0 | 78 | 939 | 862 | + alpha,alpha-trehalose-phosphate synthase (UDP-forming); Provisional | ||
TIGR02400 | trehalose_OtsA | 0 | 85 | 551 | 469 | + alpha,alpha-trehalose-phosphate synthase [UDP-forming]. This enzyme catalyzes the key, penultimate step in biosynthesis of trehalose, a compatible solute made as an osmoprotectant in some species in all three domains of life. The gene symbol OtsA stands for osmotically regulated trehalose synthesis A. Trehalose helps protect against both osmotic and thermal stresses, and is made from two glucose subunits. This model excludes glucosylglycerol-phosphate synthase, an enzyme of an analogous osmoprotectant system in many cyanobacterial strains. This model does not identify archaeal examples, as they are more divergent than glucosylglycerol-phosphate synthase. Sequences that score in the gray zone between the trusted and noise cutoffs include a number of yeast multidomain proteins in which the N-terminal domain may be functionally equivalent to this family. The gray zone also includes the OtsA of Cornyebacterium glutamicum (and related species), shown to be responsible for synthesis of only trace amounts of trehalose while the majority is synthesized by the TreYZ pathway; the significance of OtsA in this species is unclear (see Wolf, et al., PMID:12890033) [Cellular processes, Adaptations to atypical conditions]. | ||
pfam00982 | Glyco_transf_20 | 0 | 84 | 551 | 474 | + Glycosyltransferase family 20. Members of this family belong to glycosyl transferase family 20. OtsA (Trehalose-6-phosphate synthase) is homologous to regions in the subunits of yeast trehalose-6-phosphate synthase/phosphate complex. | ||
PRK14501 | PRK14501 | 0 | 85 | 812 | 735 | + putative bifunctional trehalose-6-phosphate synthase/HAD hydrolase subfamily IIB; Provisional |
Gene Ontology | |
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GO Term | Description |
GO:0003824 | catalytic activity |
GO:0005992 | trehalose biosynthetic process |
Annotations - NR Download unfiltered results here | |||||||
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Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
GenBank | ABO61742.1 | 0 | 1 | 949 | 1 | 926 | trehalose-phosphate synthase 1 [Solanum lycopersicum] |
RefSeq | NP_177979.1 | 0 | 1 | 945 | 1 | 941 | ATTPS1 (TREHALOSE-6-PHOSPHATE SYNTHASE); alpha,alpha-trehalose-phosphate synthase (UDP-forming)/ transferase, transferring glycosyl groups [Arabidopsis thaliana] |
RefSeq | XP_002285632.1 | 0 | 1 | 945 | 1 | 926 | PREDICTED: hypothetical protein [Vitis vinifera] |
RefSeq | XP_002331300.1 | 0 | 1 | 944 | 1 | 922 | predicted protein [Populus trichocarpa] |
RefSeq | XP_002531237.1 | 0 | 1 | 946 | 1 | 926 | trehalose-6-phosphate synthase, putative [Ricinus communis] |
Annotations - PDB Download unfiltered results here | |||||||
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Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
PDB | 2wtx_D | 0 | 85 | 553 | 3 | 455 | A Chain A, Insight Into The Mechanism Of Enzymatic Glycosyltransfer With Retention Through The Synthesis And Analysis Of Bisubstrate Glycomimetics Of Trehalose-6-Phosphate Synthase |
PDB | 2wtx_C | 0 | 85 | 553 | 3 | 455 | A Chain A, Insight Into The Mechanism Of Enzymatic Glycosyltransfer With Retention Through The Synthesis And Analysis Of Bisubstrate Glycomimetics Of Trehalose-6-Phosphate Synthase |
PDB | 2wtx_B | 0 | 85 | 553 | 3 | 455 | A Chain A, Insight Into The Mechanism Of Enzymatic Glycosyltransfer With Retention Through The Synthesis And Analysis Of Bisubstrate Glycomimetics Of Trehalose-6-Phosphate Synthase |
PDB | 2wtx_A | 0 | 85 | 553 | 3 | 455 | A Chain A, Insight Into The Mechanism Of Enzymatic Glycosyltransfer With Retention Through The Synthesis And Analysis Of Bisubstrate Glycomimetics Of Trehalose-6-Phosphate Synthase |
PDB | 1gz5_D | 0 | 85 | 553 | 2 | 454 | A Chain A, Trehalose-6-Phosphate Synthase. Otsa |
EST Download unfiltered results here | ||||
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Hit | Length | Start | End | EValue |
CX546763 | 315 | 61 | 374 | 0 |
CV711307 | 294 | 82 | 375 | 0 |
DW069910 | 288 | 171 | 458 | 0 |
ES813375 | 381 | 446 | 826 | 0 |
CB651133 | 281 | 111 | 391 | 0 |
Sequence Alignments (This image is cropped. Click for full image.) |
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