y
Basic Information | |
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Species | Malus domestica |
Cazyme ID | MDP0000149570 |
Family | GH32 |
Protein Properties | Length: 665 Molecular Weight: 73921.9 Isoelectric Point: 4.4865 |
Chromosome | Chromosome/Scaffold: 010572241 Start: 579 End: 4716 |
Description | Glycosyl hydrolases family 32 protein |
View CDS |
External Links |
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NCBI Taxonomy |
CAZyDB |
Signature Domain Download full data set without filtering | |||
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Family | Start | End | Evalue |
GH32 | 155 | 461 | 0 |
TGPLFYKGWYHLFYQYNPDSAVWGNITWGHAVSTDMIHWLYLPLAMVPXRWYDANGVWTGSATILPNGEIMILYTGSTNDSVQVQNLAYPANLSDPLLLD WIKYXGNPVLTPPSGIGSTDFRDPTTAWIGPDGKWRITIGSKINTTGISMVYTTTDFINYELHDGVLHEVPGTGMWECVDFYPVSINGTKGVETSVNNDG VKHVLKASLDDTKLDHYAIGTYFIENETWVPDDPTIDVGIGLRYDYGRYYASKTFYDQNKERRILWGWINETDTATDDLEKGWSSLQTIPRTVLFDSKTG TNLLQWP |
Full Sequence |
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Protein Sequence Length: 665 Download |
MDSNNTSYTP LPGEDPSAAV PPPTHRRPXK VFAVTFSSVI FLLSLVTLII HQGPGAPPKT 60 VPEQPDHDHR HQQPAASTSP EPRSFSVPRG KLEGVSAKSS PYFSEEASYN WTNAMFSWQR 120 TAFHFQPEHN WMNEYPSKAL CFVSCGPVNQ AGNWTGPLFY KGWYHLFYQY NPDSAVWGNI 180 TWGHAVSTDM IHWLYLPLAM VPXRWYDANG VWTGSATILP NGEIMILYTG STNDSVQVQN 240 LAYPANLSDP LLLDWIKYXG NPVLTPPSGI GSTDFRDPTT AWIGPDGKWR ITIGSKINTT 300 GISMVYTTTD FINYELHDGV LHEVPGTGMW ECVDFYPVSI NGTKGVETSV NNDGVKHVLK 360 ASLDDTKLDH YAIGTYFIEN ETWVPDDPTI DVGIGLRYDY GRYYASKTFY DQNKERRILW 420 GWINETDTAT DDLEKGWSSL QTIPRTVLFD SKTGTNLLQW PVEEIEDLRL NSTEFTDVLV 480 EAGTTVPLDI GTATQLDILV DFEIELLGTE ESVNGSSGCG DGAADRSTFG PFGILALADE 540 TLSEFTPVYF RVTNSTDGDV TTYFCADELR SSRAPEVFKQ VYGGEVPVLD GETYSARVLV 600 DHSILESYAQ GGRTVISSRV YPTEAIYGAA RLFLFNNATG MNVKATLKIW QLNSAFIHPF 660 PLDQV 720 |
Functional Domains Download unfiltered results here | ||||||||
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Cdd ID | Domain | E-Value | Start | End | Length | Domain Description | ||
TIGR01322 | scrB_fam | 1.0e-42 | 120 | 622 | 533 | + sucrose-6-phosphate hydrolase. [Energy metabolism, Biosynthesis and degradation of polysaccharides]. | ||
COG1621 | SacC | 1.0e-60 | 120 | 654 | 556 | + Beta-fructosidases (levanase/invertase) [Carbohydrate transport and metabolism] | ||
cd08996 | GH32_B_Fructosidase | 2.0e-84 | 156 | 464 | 321 | + Glycosyl hydrolase family 32, beta-fructosidases. Glycosyl hydrolase family GH32 cleaves sucrose into fructose and glucose via beta-fructofuranosidase activity, producing invert sugar that is a mixture of dextrorotatory D-glucose and levorotatory D-fructose, thus named invertase (EC 3.2.1.26). This family also contains other fructofuranosidases such as inulinase (EC 3.2.1.7), exo-inulinase (EC 3.2.1.80), levanase (EC 3.2.1.65), and transfructosidases such sucrose:sucrose 1-fructosyltransferase (EC 2.4.1.99), fructan:fructan 1-fructosyltransferase (EC 2.4.1.100), sucrose:fructan 6-fructosyltransferase (EC 2.4.1.10), fructan:fructan 6G-fructosyltransferase (EC 2.4.1.243) and levan fructosyltransferases (EC 2.4.1.-). These retaining enzymes (i.e. they retain the configuration at anomeric carbon atom of the substrate) catalyze hydrolysis in two steps involving a covalent glycosyl enzyme intermediate: an aspartate located close to the N-terminus acts as the catalytic nucleophile and a glutamate acts as the general acid/base; a conserved aspartate residue in the Arg-Asp-Pro (RDP) motif stabilizes the transition state. These enzymes are predicted to display a 5-fold beta-propeller fold as found for GH43 and CH68. The breakdown of sucrose is widely used as a carbon or energy source by bacteria, fungi, and plants. Invertase is used commercially in the confectionery industry, since fructose has a sweeter taste than sucrose and a lower tendency to crystallize. A common structural feature of all these enzymes is a 5-bladed beta-propeller domain, similar to GH43, that contains the catalytic acid and catalytic base. A long V-shaped groove, partially enclosed at one end, forms a single extended substrate-binding surface across the face of the propeller. | ||
pfam00251 | Glyco_hydro_32N | 8.0e-140 | 124 | 461 | 346 | + Glycosyl hydrolases family 32 N-terminal domain. This domain corresponds to the N-terminal domain of glycosyl hydrolase family 32 which forms a five bladed beta propeller structure. | ||
smart00640 | Glyco_32 | 6.0e-175 | 124 | 612 | 499 | + Glycosyl hydrolases family 32. |
Gene Ontology | |
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GO Term | Description |
GO:0004564 | beta-fructofuranosidase activity |
GO:0004575 | sucrose alpha-glucosidase activity |
Annotations - NR Download unfiltered results here | |||||||
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Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
GenBank | AAL05427.2 | 0 | 1 | 665 | 1 | 636 | vacuolar acid invertase [Prunus cerasus] |
GenBank | ACJ04702.1 | 0 | 8 | 660 | 13 | 629 | invertase 2 [Cucumis melo] |
DDBJ | BAF35858.1 | 0 | 3 | 665 | 4 | 645 | Soluble acid invertase [Pyrus pyrifolia var. culta] |
DDBJ | BAG30919.1 | 0 | 3 | 665 | 4 | 645 | soluble acid invertase [Pyrus pyrifolia] |
RefSeq | XP_002510944.1 | 0 | 14 | 664 | 19 | 638 | Beta-fructofuranosidase, soluble isoenzyme I precursor, putative [Ricinus communis] |
Annotations - PDB Download unfiltered results here | |||||||
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Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
PDB | 3ugh_B | 0 | 109 | 660 | 8 | 544 | A Chain A, Characterization And Engineering Of The Bifunctional N- And O-glucosyltransferase Involved In Xenobiotic Metabolism In Plants |
PDB | 3ugh_A | 0 | 109 | 660 | 8 | 544 | A Chain A, Characterization And Engineering Of The Bifunctional N- And O-glucosyltransferase Involved In Xenobiotic Metabolism In Plants |
PDB | 3ugg_B | 0 | 109 | 660 | 8 | 544 | A Chain A, Characterization And Engineering Of The Bifunctional N- And O-glucosyltransferase Involved In Xenobiotic Metabolism In Plants |
PDB | 3ugg_A | 0 | 109 | 660 | 8 | 544 | A Chain A, Characterization And Engineering Of The Bifunctional N- And O-glucosyltransferase Involved In Xenobiotic Metabolism In Plants |
PDB | 3ugf_B | 0 | 109 | 660 | 8 | 544 | A Chain A, Crystal Structure Of A 6-Sst6-Sft From Pachysandra Terminalis |