| Basic Information | |
|---|---|
| Species | Malus domestica |
| Cazyme ID | MDP0000166647 |
| Family | GH32 |
| Protein Properties | Length: 219 Molecular Weight: 24326.1 Isoelectric Point: 7.2692 |
| Chromosome | Chromosome/Scaffold: 004892281 Start: 88 End: 2490 |
| Description | Glycosyl hydrolases family 32 protein |
| View CDS | |
| External Links |
|---|
| NCBI Taxonomy |
| CAZyDB |
| Signature Domain Download full data set without filtering | |||
|---|---|---|---|
| Family | Start | End | Evalue |
| GH32 | 123 | 201 | 2.5e-34 |
| PLFYKGWYHLFYQYNPDSAVWGNITWGHAVSTDMIHWLYLPLAMVPNRWYDANGVWTGSATILPNGEIMILYTGSTNDS | |||
| Full Sequence |
|---|
| Protein Sequence Length: 219 Download |
| MDSNNTSYTP LPGEDPSAAV PPPTHRRPXK VFAVTFSSVI FLLSLVTLII HQGPGAPPKT 60 VPEQPDHDHR HQQPAASTSP EPRISQQSAM LCELWTSQSS WKLDGSVRAY PTKLIPNPIQ 120 PRPLFYKGWY HLFYQYNPDS AVWGNITWGH AVSTDMIHWL YLPLAMVPNR WYDANGVWTG 180 SATILPNGEI MILYTGSTND SVPSTXPCLP RQPIGSPPP 240 |
| Functional Domains Download unfiltered results here | ||||||||
|---|---|---|---|---|---|---|---|---|
| Cdd ID | Domain | E-Value | Start | End | Length | Domain Description | ||
| TIGR01322 | scrB_fam | 6.0e-20 | 125 | 201 | 77 | + sucrose-6-phosphate hydrolase. [Energy metabolism, Biosynthesis and degradation of polysaccharides]. | ||
| COG1621 | SacC | 2.0e-23 | 111 | 201 | 93 | + Beta-fructosidases (levanase/invertase) [Carbohydrate transport and metabolism] | ||
| cd08996 | GH32_B_Fructosidase | 6.0e-36 | 123 | 200 | 78 | + Glycosyl hydrolase family 32, beta-fructosidases. Glycosyl hydrolase family GH32 cleaves sucrose into fructose and glucose via beta-fructofuranosidase activity, producing invert sugar that is a mixture of dextrorotatory D-glucose and levorotatory D-fructose, thus named invertase (EC 3.2.1.26). This family also contains other fructofuranosidases such as inulinase (EC 3.2.1.7), exo-inulinase (EC 3.2.1.80), levanase (EC 3.2.1.65), and transfructosidases such sucrose:sucrose 1-fructosyltransferase (EC 2.4.1.99), fructan:fructan 1-fructosyltransferase (EC 2.4.1.100), sucrose:fructan 6-fructosyltransferase (EC 2.4.1.10), fructan:fructan 6G-fructosyltransferase (EC 2.4.1.243) and levan fructosyltransferases (EC 2.4.1.-). These retaining enzymes (i.e. they retain the configuration at anomeric carbon atom of the substrate) catalyze hydrolysis in two steps involving a covalent glycosyl enzyme intermediate: an aspartate located close to the N-terminus acts as the catalytic nucleophile and a glutamate acts as the general acid/base; a conserved aspartate residue in the Arg-Asp-Pro (RDP) motif stabilizes the transition state. These enzymes are predicted to display a 5-fold beta-propeller fold as found for GH43 and CH68. The breakdown of sucrose is widely used as a carbon or energy source by bacteria, fungi, and plants. Invertase is used commercially in the confectionery industry, since fructose has a sweeter taste than sucrose and a lower tendency to crystallize. A common structural feature of all these enzymes is a 5-bladed beta-propeller domain, similar to GH43, that contains the catalytic acid and catalytic base. A long V-shaped groove, partially enclosed at one end, forms a single extended substrate-binding surface across the face of the propeller. | ||
| smart00640 | Glyco_32 | 3.0e-39 | 123 | 200 | 78 | + Glycosyl hydrolases family 32. | ||
| pfam00251 | Glyco_hydro_32N | 3.0e-42 | 123 | 203 | 81 | + Glycosyl hydrolases family 32 N-terminal domain. This domain corresponds to the N-terminal domain of glycosyl hydrolase family 32 which forms a five bladed beta propeller structure. | ||
| Gene Ontology | |
|---|---|
| GO Term | Description |
| GO:0004564 | beta-fructofuranosidase activity |
| GO:0004575 | sucrose alpha-glucosidase activity |
| Annotations - NR Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| GenBank | AAB47171.1 | 0 | 8 | 202 | 22 | 208 | vacuolar invertase 1, GIN1 [Vitis vinifera=grape berries, Sultana, berries, Peptide, 642 aa] |
| DDBJ | BAD91192.1 | 5.60519e-45 | 123 | 202 | 21 | 100 | soluble acid invertase [Pyrus communis] |
| DDBJ | BAF35858.1 | 0 | 3 | 202 | 4 | 216 | Soluble acid invertase [Pyrus pyrifolia var. culta] |
| DDBJ | BAG30919.1 | 0 | 3 | 202 | 4 | 216 | soluble acid invertase [Pyrus pyrifolia] |
| RefSeq | XP_002265534.1 | 1.4013e-45 | 8 | 202 | 22 | 215 | PREDICTED: hypothetical protein [Vitis vinifera] |
| Annotations - PDB Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| PDB | 3ugh_B | 2e-34 | 123 | 202 | 37 | 116 | A Chain A, Structure And Mechanisim Of Core 2 Beta1,6-N- Acetylglucosaminyltransferase: A Metal-Ion Independent Gt-A Glycosyltransferase |
| PDB | 3ugh_A | 2e-34 | 123 | 202 | 37 | 116 | A Chain A, Structure And Mechanisim Of Core 2 Beta1,6-N- Acetylglucosaminyltransferase: A Metal-Ion Independent Gt-A Glycosyltransferase |
| PDB | 3ugg_B | 2e-34 | 123 | 202 | 37 | 116 | A Chain A, Structure And Mechanisim Of Core 2 Beta1,6-N- Acetylglucosaminyltransferase: A Metal-Ion Independent Gt-A Glycosyltransferase |
| PDB | 3ugg_A | 2e-34 | 123 | 202 | 37 | 116 | A Chain A, Structure And Mechanisim Of Core 2 Beta1,6-N- Acetylglucosaminyltransferase: A Metal-Ion Independent Gt-A Glycosyltransferase |
| PDB | 3ugf_B | 2e-34 | 123 | 202 | 37 | 116 | A Chain A, Crystal Structure Of A 6-Sst6-Sft From Pachysandra Terminalis |
