| Basic Information | |
|---|---|
| Species | Malus domestica |
| Cazyme ID | MDP0000182058 |
| Family | GH16 |
| Protein Properties | Length: 299 Molecular Weight: 34774.5 Isoelectric Point: 4.5843 |
| Chromosome | Chromosome/Scaffold: 007611496 Start: 12854 End: 14685 |
| Description | xyloglucan endotransglucosylase/hydrolase 8 |
| View CDS | |
| External Links |
|---|
| NCBI Taxonomy |
| CAZyDB |
| Signature Domain Download full data set without filtering | |||
|---|---|---|---|
| Family | Start | End | Evalue |
| GH16 | 56 | 216 | 9.1e-24 |
| WYLSLDKDTGCGFQTKQKYRFGWFSMKIKLVGGDSAGVVTAYYMCSENGAGPERDELDFEFLGNRTGQPYLIQTNVFKNGTGNREMRHQLWFDPTEDYHS YSILWNNHQIVFFVDKVPIRVYKNNGEANNFFPNEKPMYLFSSIWNADSWATRGGLEKTDW | |||
| Full Sequence |
|---|
| Protein Sequence Length: 299 Download |
| MERRAFPMPI LLYXAVFIAA SCSSSEAAAT TSSFDDNFSI MWSENHFTTS ADKQIWYLSL 60 DKDTGCGFQT KQKYRFGWFS MKIKLVGGDS AGVVTAYYMC SENGAGPERD ELDFEFLGNR 120 TGQPYLIQTN VFKNGTGNRE MRHQLWFDPT EDYHSYSILW NNHQIVFFVD KVPIRVYKNN 180 GEANNFFPNE KPMYLFSSIW NADSWATRGG LEKTDWTKAP FVSSYTEFSV DACQWEDPFP 240 ACVSTTTDNW WDQYNAWHLS DDQKKDYAWI QRNMVIYDYC KDSERFPTLP VECPLSPWE 300 |
| Functional Domains Download unfiltered results here | ||||||||
|---|---|---|---|---|---|---|---|---|
| Cdd ID | Domain | E-Value | Start | End | Length | Domain Description | ||
| cd02175 | GH16_lichenase | 5.0e-18 | 35 | 206 | 184 | + lichenase, member of glycosyl hydrolase family 16. Lichenase, also known as 1,3-1,4-beta-glucanase, is a member of glycosyl hydrolase family 16, that specifically cleaves 1,4-beta-D-glucosidic bonds in mixed-linked beta glucans that also contain 1,3-beta-D-glucosidic linkages. Natural substrates of beta-glucanase are beta-glucans from grain endosperm cell walls or lichenan from the Islandic moss, Cetraria islandica. This protein is found not only in bacteria but also in anaerobic fungi. This domain includes two seven-stranded antiparallel beta-sheets that are adjacent to one another forming a compact, jellyroll beta-sandwich structure. | ||
| cd02183 | GH16_fungal_CRH1_transglycosylase | 1.0e-20 | 76 | 221 | 159 | + glycosylphosphatidylinositol-glucanosyltransferase. Group of fungal GH16 members related to Saccharomyces cerevisiae Crh1p. Chr1p and Crh2p are transglycosylases that are required for the linkage of chitin to beta(1-3)glucose branches of beta(1-6)glucan, an important step in the assembly of new cell wall. Both have been shown to be glycosylphosphatidylinositol (GPI)-anchored. A third homologous protein, Crr1p, functions in the formation of the spore wall. They belongs to the family 16 of glycosyl hydrolases that includes lichenase, xyloglucan endotransglycosylase (XET), beta-agarase, kappa-carrageenase, endo-beta-1,3-glucanase, endo-beta-1,3-1,4-glucanase, and endo-beta-galactosidase, all of which have a conserved jelly roll fold with a deep active site channel harboring the catalytic residues. | ||
| pfam00722 | Glyco_hydro_16 | 2.0e-66 | 34 | 218 | 186 | + Glycosyl hydrolases family 16. | ||
| PLN03161 | PLN03161 | 8.0e-83 | 34 | 293 | 263 | + Probable xyloglucan endotransglucosylase/hydrolase protein; Provisional | ||
| cd02176 | GH16_XET | 8.0e-149 | 34 | 293 | 260 | + Xyloglucan endotransglycosylase, member of glycosyl hydrolase family 16. Xyloglucan endotransglycosylases (XETs) cleave and religate xyloglucan polymers in plant cell walls via a transglycosylation mechanism. Xyloglucan is a soluble hemicellulose with a backbone of beta-1,4-linked glucose units, partially substituted with alpha-1,6-linked xylopyranose branches. It binds noncovalently to cellulose, cross-linking the adjacent cellulose microfibrils, giving it a key structural role as a matrix polymer. Therefore, XET plays an important role in all plant processes that require cell wall remodeling. | ||
| Gene Ontology | |
|---|---|
| GO Term | Description |
| GO:0004553 | hydrolase activity, hydrolyzing O-glycosyl compounds |
| GO:0005618 | cell wall |
| GO:0005975 | carbohydrate metabolic process |
| GO:0006073 | cellular glucan metabolic process |
| GO:0016762 | xyloglucan:xyloglucosyl transferase activity |
| Annotations - NR Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| GenBank | ABK95902.1 | 0 | 21 | 299 | 15 | 293 | unknown [Populus trichocarpa] |
| GenBank | ACD03227.1 | 0 | 1 | 299 | 1 | 300 | xyloglucan endotransglucosylase/hydrolase 3 [Malus x domestica] |
| GenBank | ACU21152.1 | 0 | 10 | 299 | 13 | 302 | unknown [Glycine max] |
| RefSeq | XP_002304928.1 | 0 | 21 | 298 | 17 | 294 | predicted protein [Populus trichocarpa] |
| RefSeq | XP_002518528.1 | 0 | 1 | 299 | 1 | 300 | Xyloglucan endotransglucosylase/hydrolase protein A precursor, putative [Ricinus communis] |
| Annotations - PDB Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| PDB | 1un1_B | 0 | 33 | 293 | 16 | 272 | A Chain A, Hevamine Mutant D125aY183F IN COMPLEX WITH TETRA-Nag |
| PDB | 1un1_A | 0 | 33 | 293 | 16 | 272 | A Chain A, Hevamine Mutant D125aY183F IN COMPLEX WITH TETRA-Nag |
| PDB | 1umz_B | 0 | 33 | 293 | 16 | 272 | A Chain A, Xyloglucan Endotransglycosylase In Complex With The Xyloglucan Nonasaccharide Xllg. |
| PDB | 1umz_A | 0 | 33 | 293 | 16 | 272 | A Chain A, Xyloglucan Endotransglycosylase In Complex With The Xyloglucan Nonasaccharide Xllg. |
| PDB | 2vh9_B | 0 | 8 | 293 | 13 | 290 | A Chain A, Crystal Structure Of Nxg1-Deltayniig In Complex With Xllg, A Xyloglucan Derived Oligosaccharide |
