Basic Information | |
---|---|
Species | Malus domestica |
Cazyme ID | MDP0000218204 |
Family | CBM20 |
Protein Properties | Length: 390 Molecular Weight: 43789 Isoelectric Point: 4.8525 |
Chromosome | Chromosome/Scaffold: 021098161 Start: 8441 End: 10362 |
Description | Carbohydrate-binding-like fold |
View CDS |
External Links |
---|
NCBI Taxonomy |
CAZyDB |
Signature Domain Download full data set without filtering | |||
---|---|---|---|
Family | Start | End | Evalue |
CBM20 | 95 | 173 | 3.7e-24 |
QKECSFGQEFLIVGDDPMFGLWDPASAIPMIWSDGNVWTVELDIPVGKXIQFKFILKESTGNISWQPGPDRIFQTWETR |
Full Sequence |
---|
Protein Sequence Length: 390 Download |
MRTLSSPSSR VAVEKKWVGR ENVFPRELEP CFLRPRRSGN LRFLNLVCFL KQSKPVIRPI 60 ICLSSKTQTV NLISEHSLCR QNWRLQILKF RKHLQKECSF GQEFLIVGDD PMFGLWDPAS 120 AIPMIWSDGN VWTVELDIPV GKXIQFKFIL KESTGNISWQ PGPDRIFQTW ETRNRITVSE 180 DWADAELQKI IEEDRVSNQS DGSNVNADML IMDENLTQXN GELAFNTTNX PKITNSNTDS 240 AENPSVEPRK EXIGADNGSP SQENMTPKWP KAYVEPYKER VAARTAVLNE ERVIFPSXDS 300 AAISSKELLV ADNIFGNNGR AATVMNLTST HIEGSLVNXE EDPVLVPGLT PSPAVPNEEV 360 NKEGIEKQMS FDGSVGAFEA KDRNMPEVTA |
Functional Domains Download unfiltered results here | ||||||||
---|---|---|---|---|---|---|---|---|
Cdd ID | Domain | E-Value | Start | End | Length | Domain Description | ||
cd02861 | E_set_pullulanase_like | 2.0e-14 | 88 | 177 | 92 | + Early set domain associated with the catalytic domain of pullulanase-like proteins. E or "early" set domains are associated with the catalytic domain of pullulanase at either the N-terminal or C-terminal end, and in a few instances at both ends. Pullulanase (also called dextrinase or alpha-dextrin endo-1,6-alpha glucosidase) is an enzyme with action similar to that of isoamylase; it cleaves 1,6-alpha-glucosidic linkages in pullulan, amylopectin, and glycogen, and in alpha-and beta-amylase limit-dextrins of amylopectin and glycogen. The E set domain of pullulanase may be related to the immunoglobulin and/or fibronectin type III superfamilies. These domains are associated with different types of catalytic domains at either the N-terminal or C-terminal end and may be involved in homodimeric/tetrameric/dodecameric interactions. Members of this family include members of the alpha amylase family, sialidase, galactose oxidase, cellulase, cellulose, hyaluronate lyase, chitobiase, and chitinase. This domain is also a member of the CBM48 (Carbohydrate Binding Module 48) family whose members include maltooligosyl trehalose synthase, starch branching enzyme, glycogen branching enzyme, glycogen debranching enzyme, isoamylase, and the beta subunit of AMP-activated protein kinase. | ||
cd02853 | E_set_MTHase_like_N | 2.0e-15 | 100 | 169 | 70 | + N-terminal Early set domain associated with the catalytic domain of Maltooligosyl trehalose trehalohydrolase (also called Glycosyltrehalose trehalohydrolase) and similar proteins. E or "early" set domains are associated with the catalytic domain of Maltooligosyl trehalose trehalohydrolase (MTHase) and similar proteins at the N-terminal end. This subfamily also includes bacterial alpha amylases and 1,4-alpha-glucan branching enzymes which are highly similar to MTHase. Maltooligosyl trehalose synthase (MTSase) and MTHase work together to produce trehalose. MTSase is responsible for converting the alpha-1,4-glucosidic linkage to an alpha,alpha-1,1-glucosidic linkage at the reducing end of the maltooligosaccharide through an intramolecular transglucosylation reaction, while MTHase hydrolyzes the penultimate alpha-1,4 linkage of the reducing end, resulting in the release of trehalose. The N-terminal domain of MTHase may be related to the immunoglobulin and/or fibronectin type III superfamilies. These domains are associated with different types of catalytic domains at either the N-terminal or C-terminal end and may be involved in homodimeric/tetrameric/dodecameric interactions. Members of this family include members of the alpha amylase family, sialidase, galactose oxidase, cellulase, cellulose, hyaluronate lyase, chitobiase, and chitinase, among others. | ||
smart01065 | CBM_2 | 4.0e-16 | 99 | 171 | 76 | + Starch binding domain. | ||
pfam00686 | CBM_20 | 1.0e-18 | 99 | 172 | 77 | + Starch binding domain. | ||
cd05467 | CBM20 | 2.0e-24 | 87 | 182 | 99 | + The family 20 carbohydrate-binding module (CBM20), also known as the starch-binding domain, is found in a large number of starch degrading enzymes including alpha-amylase, beta-amylase, glucoamylase, and CGTase (cyclodextrin glucanotransferase). CBM20 is also present in proteins that have a regulatory role in starch metabolism in plants (e.g. alpha-amylase) or glycogen metabolism in mammals (e.g. laforin). CBM20 folds as an antiparallel beta-barrel structure with two starch binding sites. These two sites are thought to differ functionally with site 1 acting as the initial starch recognition site and site 2 involved in the specific recognition of appropriate regions of starch. |
Gene Ontology | |
---|---|
GO Term | Description |
GO:0003824 | catalytic activity |
GO:0005975 | carbohydrate metabolic process |
GO:2001070 | starch binding |
Annotations - NR Download unfiltered results here | |||||||
---|---|---|---|---|---|---|---|
Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
GenBank | ACU23169.1 | 0 | 86 | 389 | 91 | 391 | unknown [Glycine max] |
EMBL | CAN60108.1 | 0 | 32 | 387 | 7 | 352 | hypothetical protein [Vitis vinifera] |
EMBL | CBI30404.1 | 0 | 1 | 249 | 1 | 243 | unnamed protein product [Vitis vinifera] |
RefSeq | XP_002308216.1 | 0 | 86 | 385 | 28 | 315 | predicted protein [Populus trichocarpa] |
RefSeq | XP_002528119.1 | 0 | 83 | 390 | 9 | 323 | catalytic, putative [Ricinus communis] |
Annotations - PDB Download unfiltered results here | |||||||
---|---|---|---|---|---|---|---|
Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
PDB | 1cyg_A | 0.00000003 | 98 | 182 | 591 | 678 | A Chain A, Cyclodextrin Glucanotransferase (E.C.2.4.1.19) (Cgtase) |
PDB | 1v3m_B | 0.00001 | 98 | 182 | 598 | 684 | A Chain A, Cyclodextrin Glucanotransferase (E.C.2.4.1.19) (Cgtase) |
PDB | 1v3m_A | 0.00001 | 98 | 182 | 598 | 684 | A Chain A, Cyclodextrin Glucanotransferase (E.C.2.4.1.19) (Cgtase) |
PDB | 1v3l_B | 0.00001 | 98 | 182 | 598 | 684 | A Chain A, Cyclodextrin Glucanotransferase (E.C.2.4.1.19) (Cgtase) |
PDB | 1v3l_A | 0.00001 | 98 | 182 | 598 | 684 | A Chain A, Cyclodextrin Glucanotransferase (E.C.2.4.1.19) (Cgtase) |
EST Download unfiltered results here | ||||
---|---|---|---|---|
Hit | Length | Start | End | EValue |
EB150095 | 192 | 140 | 331 | 0 |
CN492262 | 197 | 118 | 314 | 0 |
DT003352 | 205 | 1 | 204 | 0 |
EB148134 | 165 | 140 | 304 | 0 |
CN915421 | 181 | 1 | 181 | 0 |
Sequence Alignments (This image is cropped. Click for full image.) |
---|