Basic Information | |
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Species | Malus domestica |
Cazyme ID | MDP0000218396 |
Family | GH13 |
Protein Properties | Length: 669 Molecular Weight: 75300 Isoelectric Point: 6.2881 |
Chromosome | Chromosome/Scaffold: 020208387 Start: 411 End: 6400 |
Description | starch branching enzyme 2.1 |
View CDS |
External Links |
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NCBI Taxonomy |
CAZyDB |
Signature Domain Download full data set without filtering | |||
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Family | Start | End | Evalue |
GH13 | 459 | 607 | 8.7e-28 |
LPRIKKLGYNAVQLMAIQEHSYYASFGYHVTNFFAPSSRCGTPDDLKSLIDKAHELGLLVLMDIVHSHASNNTLDGLNMFDGTDSHYFHSGSRGYHWMWD SRLFNYGSWEVLRYLLSNARWWLEEYKFDGFRFDGVTSMMYTHHGLEVA |
Full Sequence |
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Protein Sequence Length: 669 Download |
MDDRKFRGEA DWVPPTHPHA TLNCTRGIQR YSTKYLVTLT PPSLSPPDFS VASLTHSTFL 60 PGGEFRNSAK RNGCCCCFEI DRSFSRASVD LITKGGRRNG LDSLGDXLPS PTFRLHLAFQ 120 LQWRSEDLRP LSLPLQHLLL SYSLSLRKIF VGKPSYDSNL PSLAVXASKK MLVPDSQSDG 180 SSSLKKEQSG AASTVPEDPQ VIQDVDNVAK EDXEKLEDAP SLVVANVDDA EAKVEDTPRP 240 LEVKASTATN KATGKTIPPP GNGQKIYEID SLLVGHRDHL DYRYXQYKRL REEIXKYEGG 300 LEVFSRGYEK FGFTRSAXGI TYREWAPGAK SASLIGDFNN WNTNADVMTQ NDXGVWEIFL 360 PNNADGSPAI PHGSRVKVRM DTPSGIKDSI PAWIKFSIQA PGEIPYNGIY YDPPEEEKYV 420 FQHSQPSRPK SLRIYEAHVG MSSTEPKINT YAEFRDDVLP RIKKLGYNAV QLMAIQEHSY 480 YASFGYHVTN FFAPSSRCGT PDDLKSLIDK AHELGLLVLM DIVHSHASNN TLDGLNMFDG 540 TDSHYFHSGS RGYHWMWDSR LFNYGSWEVL RYLLSNARWW LEEYKFDGFR FDGVTSMMYT 600 HHGLEVAFTG NYREYFGLAT DVDAVTYLML VNDLIHGLYP EAVTIGEDVC CLFPFISKVE 660 HPLLQLSPK |
Functional Domains Download unfiltered results here | ||||||||
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Cdd ID | Domain | E-Value | Start | End | Length | Domain Description | ||
PLN02960 | PLN02960 | 4.0e-9 | 283 | 360 | 84 | + alpha-amylase | ||
COG0296 | GlgB | 4.0e-86 | 308 | 648 | 348 | + 1,4-alpha-glucan branching enzyme [Carbohydrate transport and metabolism] | ||
PLN02960 | PLN02960 | 6.0e-113 | 368 | 654 | 288 | + alpha-amylase | ||
cd11321 | AmyAc_bac_euk_BE | 2.0e-170 | 415 | 649 | 235 | + Alpha amylase catalytic domain found in bacterial and eukaryotic branching enzymes. Branching enzymes (BEs) catalyze the formation of alpha-1,6 branch points in either glycogen or starch by cleavage of the alpha-1,4 glucosidic linkage yielding a non-reducing end oligosaccharide chain, and subsequent attachment to the alpha-1,6 position. By increasing the number of non-reducing ends, glycogen is more reactive to synthesis and digestion as well as being more soluble. This group includes bacterial and eukaryotic proteins. The Alpha-amylase family comprises the largest family of glycoside hydrolases (GH), with the majority of enzymes acting on starch, glycogen, and related oligo- and polysaccharides. These proteins catalyze the transformation of alpha-1,4 and alpha-1,6 glucosidic linkages with retention of the anomeric center. The protein is described as having 3 domains: A, B, C. A is a (beta/alpha) 8-barrel; B is a loop between the beta 3 strand and alpha 3 helix of A; C is the C-terminal extension characterized by a Greek key. The majority of the enzymes have an active site cleft found between domains A and B where a triad of catalytic residues (Asp, Glu and Asp) performs catalysis. Other members of this family have lost the catalytic activity as in the case of the human 4F2hc, or only have 2 residues that serve as the catalytic nucleophile and the acid/base, such as Thermus A4 beta-galactosidase with 2 Glu residues (GH42) and human alpha-galactosidase with 2 Asp residues (GH31). The family members are quite extensive and include: alpha amylase, maltosyltransferase, cyclodextrin glycotransferase, maltogenic amylase, neopullulanase, isoamylase, 1,4-alpha-D-glucan maltotetrahydrolase, 4-alpha-glucotransferase, oligo-1,6-glucosidase, amylosucrase, sucrose phosphorylase, and amylomaltase. | ||
PLN02447 | PLN02447 | 0 | 218 | 649 | 435 | + 1,4-alpha-glucan-branching enzyme |
Gene Ontology | |
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GO Term | Description |
GO:0003824 | catalytic activity |
GO:0004553 | hydrolase activity, hydrolyzing O-glycosyl compounds |
GO:0005975 | carbohydrate metabolic process |
GO:0043169 | cation binding |
Annotations - NR Download unfiltered results here | |||||||
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Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
GenBank | ABO31358.1 | 0 | 138 | 661 | 35 | 557 | starch branching enzyme II-1 [Malus x domestica] |
GenBank | ABO31359.1 | 0 | 138 | 660 | 34 | 556 | starch branching enzyme II-2 [Malus x domestica] |
GenBank | ACA35282.1 | 0 | 133 | 660 | 30 | 586 | starch branching enzyme I [Cucumis sativus] |
GenBank | ACA35286.1 | 0 | 133 | 660 | 30 | 586 | starch branching enzyme I [Cucumis sativus] |
EMBL | CBI30261.1 | 0 | 147 | 660 | 44 | 568 | unnamed protein product [Vitis vinifera] |
Annotations - PDB Download unfiltered results here | |||||||
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Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
PDB | 3amk_A | 0 | 266 | 652 | 13 | 404 | A Chain A, Structure Of The Starch Branching Enzyme I (Bei) From Oryza Sativa L |
PDB | 3vu2_B | 0 | 266 | 652 | 13 | 404 | A Chain A, Structure Of The Starch Branching Enzyme I (bei) Complexed With Maltopentaose From Oryza Sativa L |
PDB | 3vu2_A | 0 | 266 | 652 | 13 | 404 | A Chain A, Structure Of The Starch Branching Enzyme I (bei) Complexed With Maltopentaose From Oryza Sativa L |
PDB | 3aml_A | 0 | 266 | 652 | 13 | 404 | A Chain A, Structure Of The Starch Branching Enzyme I (Bei) From Oryza Sativa L |
PDB | 3k1d_A | 1e-39 | 319 | 648 | 137 | 456 | A Chain A, Crystal Structure Of Glycogen Branching Enzyme Synonym: 1,4- Glucan:1,4-Alpha-D-Glucan 6-Glucosyl-Transferase From Mycob Tuberculosis H3 |
EST Download unfiltered results here | ||||
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Hit | Length | Start | End | EValue |
HO794536 | 396 | 260 | 655 | 0 |
HO777638 | 340 | 316 | 655 | 0 |
DY669254 | 264 | 286 | 549 | 0 |
HO458123 | 296 | 260 | 548 | 0 |
HO777638 | 47 | 269 | 315 | 0.000000001 |
Sequence Alignments (This image is cropped. Click for full image.) |
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