| Basic Information | |
|---|---|
| Species | Malus domestica |
| Cazyme ID | MDP0000231077 |
| Family | GT24 |
| Protein Properties | Length: 1801 Molecular Weight: 203218 Isoelectric Point: 5.966 |
| Chromosome | Chromosome/Scaffold: 016992159 Start: 2001 End: 21537 |
| Description | UDP-glucose:glycoprotein glucosyltransferases;transferases, transferring hexosyl groups;transferases, transferring glycosyl groups |
| View CDS | |
| External Links |
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| NCBI Taxonomy |
| CAZyDB |
| Signature Domain Download full data set without filtering | |||
|---|---|---|---|
| Family | Start | End | Evalue |
| GT24 | 1467 | 1742 | 0 |
| INIFSIASGHLYERFLKIMILSVLKNTXRPVKFWFIKNYLSPQFKDVIPPMAQEYGFEYELITYKWPTWLHKQKEKQRIIWAYKILFLDVIFPLSLEKVI FVDADQIVRADMGELYDMDIKGRPLAYTPFCDNNKDMDGYRFWRQGFWKEHLRGRSYHIRKTAGVDMLILCSALYVVDLKKFRETAAGDNLRVFYETLSK DPNSLSNLDQASGHVSNSTLTTEAQXDLPNYAQHTVPIFSLPQEWLWCESWCGNATKSKAKTIDLCNNPMTKEPKL | |||
| Full Sequence |
|---|
| Protein Sequence Length: 1801 Download |
| MRTRFKSAFC AVIVLVCLGA SGIGLVSGQN XRPKNVQAAV RAKWSGTPLL LEAGELLSKE 60 QKDHFWDFID AWHHSEKDDA ESYTAKGCLK KIVKHGLSIL NEPLASLFEF SLMLRSTSPR 120 LVLYRQLAEE ALSSFPLVDE TNSSSDSGIS ETNELMEGKR SDLLNIGRNP KSPNGKCCWV 180 DTGGALFFDP ADLKIWLQSP RDSSGDSFQQ PELFEFDHIH FDSSVGSPVA VLYGALGTDC 240 FREFHLTLVE AAKEIVRSFS GKTSRMATPV FELLPGRFGW MLLSSGRMFG LAQNFQGKAK 300 YVVRQVLPSG CDXKIDRCGA VGTRDSLNLG GYGVELALKN MEYKAMDDSA IKKAFEACPX 360 ILRMFECKKR NRFFVLGQNI PQKYXVYVAG VTLEDPRIED LSQEVRGFIF SKILERKPEL 420 SSEIMAFRDY LLSSTISDTL DVWELKDLGH QTAQRIVQAS DPLQAMQEIN QNFPSIVSSL 480 SRMKLNDSVK DEISANQRMX PPGKSLMALN GALLNIEDID LYLLLDLVHQ DLSLADQFSK 540 LKSTLGTLGL IPHGTIRKLL ASLPPPESNM LRVDFRSDHV QYLNDIEEDD MYRRWRSNLN 600 EILMPVFPGQ LRYIRKNLFH AVSVIDPSTV CGLQSIDMIL SLYENNFPMR FGVVLYSSKF 660 IKQIETRGSE DDHEIEEDMS SLIIRLFIYI KENHGIQTAF QFLSNINKLR IDSEDSADDA 720 LEMHHVEGAF IETVLPNTKS PPQDLLLKLE KEQTFKELSQ ESSMFVFKLG LAKLQCCLLM 780 NGLVLESNEE ALINSMNDEL PRIQEQVYYG HINSRTDVLD KFLSESXTTR YNPQIIAGGK 840 PRFISLPTSI LGGDGVLNDI NYLHSPETMD DLKPVTHLLA VXIASKKGMK LLHEGLQYLI 900 EASNRARVGV LFSVNQDADV SSHLFVKVFE ITASSYSHKK KVLDFLDQMC SFYEHNYLLA 960 SSKGTKSTQE FIDKVCELAE ANGLSSKAYR FSLSEFSDEX LRKSMNKVSQ FLYRQLGLES 1020 GVNAVITNGR VTVVNDGGTF LSHDLRLLES LEFAQRIKHI VEIIEEVKWE DMDPDILTSR 1080 TMCTTDYNSL KFSFFHPSSM XLEDPSSVAN TLGKFISDTI MSVSSXMAMR DRSSESARFE 1140 VLSAQYSAIV LNNENASIHI DAVIDPLSPF GQKLSSILRV LWKYTQPSMR IVLNPLSSLV 1200 DLPLKNYYRY VLPTVDDFSS TDYTINGPKA FFANMPLSKT LTMNLDVPDP WLVEPVIAVH 1260 DLDNILLENL GETRTLQAVF ELEALVLTVN GKVRVTNYVV ICTDKSSTGH CSEKGHDHPR 1320 GLQLIIGTKS TPHLVDTLVM ANLGYWQMKV SPGVWYLQLA PGRSSELYFL KEDGNESGSK 1380 TLSKRITIDD LRGKVVHMEV AKKKGKEHEK LLVPDGEDNS HDXKEGSSWN SNFLKWASGF 1440 IGGGEQSKKS ESTSAEQGKG GRHGKTINIF SIASGHLYER FLKIMILSVL KNTXRPVKFW 1500 FIKNYLSPQF KDVIPPMAQE YGFEYELITY KWPTWLHKQK EKQRIIWAYK ILFLDVIFPL 1560 SLEKVIFVDA DQIVRADMGE LYDMDIKGRP LAYTPFCDNN KDMDGYRFWR QGFWKEHLRG 1620 RSYHIRKTAG VDMLILCSAL YVVDLKKFRE TAAGDNLRVF YETLSKDPNS LSNLDQASGH 1680 VSNSTLTTEA QXDLPNYAQH TVPIFSLPQE WLWCESWCGN ATKSKAKTID LCNNPMTKEP 1740 KLQGARRIVS EWPDLDLEAR QFTAKILGDE LDIQEPAPLP NEPEKSVTGS PEEDLESKAE 1800 L 1860 |
| Functional Domains Download unfiltered results here | ||||||||
|---|---|---|---|---|---|---|---|---|
| Cdd ID | Domain | E-Value | Start | End | Length | Domain Description | ||
| pfam01501 | Glyco_transf_8 | 2.0e-5 | 1471 | 1676 | 213 | + Glycosyl transferase family 8. This family includes enzymes that transfer sugar residues to donor molecules. Members of this family are involved in lipopolysaccharide biosynthesis and glycogen synthesis. This family includes Lipopolysaccharide galactosyltransferase, lipopolysaccharide glucosyltransferase 1, and glycogenin glucosyltransferase. | ||
| cd04194 | GT8_A4GalT_like | 8.0e-14 | 1467 | 1676 | 218 | + A4GalT_like proteins catalyze the addition of galactose or glucose residues to the lipooligosaccharide (LOS) or lipopolysaccharide (LPS) of the bacterial cell surface. The members of this family of glycosyltransferases catalyze the addition of galactose or glucose residues to the lipooligosaccharide (LOS) or lipopolysaccharide (LPS) of the bacterial cell surface. The enzymes exhibit broad substrate specificities. The known functions found in this family include: Alpha-1,4-galactosyltransferase, LOS-alpha-1,3-D-galactosyltransferase, UDP-glucose:(galactosyl) LPS alpha1,2-glucosyltransferase, UDP-galactose: (glucosyl) LPS alpha1,2-galactosyltransferase, and UDP-glucose:(glucosyl) LPS alpha1,2-glucosyltransferase. Alpha-1,4-galactosyltransferase from N. meningitidis adds an alpha-galactose from UDP-Gal (the donor) to a terminal lactose (the acceptor) of the LOS structure of outer membrane. LOSs are virulence factors that enable the organism to evade the immune system of host cells. In E. coli, the three alpha-1,2-glycosyltransferases, that are involved in the synthesis of the outer core region of the LPS, are all members of this family. The three enzymes share 40 % of sequence identity, but have different sugar donor or acceptor specificities, representing the structural diversity of LPS. | ||
| cd00505 | Glyco_transf_8 | 1.0e-50 | 1467 | 1742 | 285 | + Members of glycosyltransferase family 8 (GT-8) are involved in lipopolysaccharide biosynthesis and glycogen synthesis. Members of this family are involved in lipopolysaccharide biosynthesis and glycogen synthesis. GT-8 comprises enzymes with a number of known activities: lipopolysaccharide galactosyltransferase, lipopolysaccharide glucosyltransferase 1, glycogenin glucosyltransferase, and N-acetylglucosaminyltransferase. GT-8 enzymes contains a conserved DXD motif which is essential in the coordination of a catalytic divalent cation, most commonly Mn2+. | ||
| pfam06427 | UDP-g_GGTase | 4.0e-73 | 1117 | 1342 | 232 | + UDP-glucose:Glycoprotein Glucosyltransferase. The N-terminal region of this group of proteins is required for correct folding of the ER UDP-Glc: glucosyltransferase. | ||
| cd06432 | GT8_HUGT1_C_like | 3.0e-167 | 1467 | 1742 | 276 | + The C-terminal domain of HUGT1-like is highly homologous to the GT 8 family. C-terminal domain of glycoprotein glucosyltransferase (UGT). UGT is a large glycoprotein whose C-terminus contains the catalytic activity. This catalytic C-terminal domain is highly homologous to Glycosyltransferase Family 8 (GT 8) and contains the DXD motif that coordinates donor sugar binding, characteristic for Family 8 glycosyltransferases. GT 8 proteins are retaining enzymes based on the relative anomeric stereochemistry of the substrate and product in the reaction catalyzed. The non-catalytic N-terminal portion of the human UTG1 (HUGT1) has been shown to monitor the protein folding status and activate its glucosyltransferase activity. | ||
| Gene Ontology | |
|---|---|
| GO Term | Description |
| GO:0003980 | UDP-glucose:glycoprotein glucosyltransferase activity |
| GO:0006486 | protein glycosylation |
| Annotations - NR Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| GenBank | AAG51883.1 | 0 | 26 | 1801 | 23 | 1674 | AC016162_4 putative UDP-glucose:glycoprotein glucosyltransferase; 101200-91134 [Arabidopsis thaliana] |
| DDBJ | BAD28382.1 | 0 | 32 | 1794 | 36 | 1612 | putative UDP-glucose:glycoprotein glucosyltransferase [Oryza sativa Japonica Group] |
| RefSeq | NP_177278.3 | 0 | 9 | 1801 | 12 | 1613 | EBS1 (EMS-mutagenized bri1 suppressor 1); UDP-glucose:glycoprotein glucosyltransferase/ transferase, transferring glycosyl groups / transferase, transferring hexosyl groups [Arabidopsis thaliana] |
| RefSeq | XP_002268972.1 | 0 | 1 | 1801 | 1 | 1611 | PREDICTED: similar to EBS1 (EMS-MUTAGENIZED BRI1 SUPPRESSOR 1); UDP-glucose:glycoprotein glucosyltransferase/ transferase, transferring glycosyl groups / transferase, transferring hexosyl groups [Vitis vinifera] |
| RefSeq | XP_002529534.1 | 0 | 1 | 1678 | 1 | 1501 | UDP-glucose glycoprotein:glucosyltransferase, putative [Ricinus communis] |
