| Basic Information | |
|---|---|
| Species | Malus domestica |
| Cazyme ID | MDP0000243097 |
| Family | GT1 |
| Protein Properties | Length: 1302 Molecular Weight: 144979 Isoelectric Point: 6.8517 |
| Chromosome | Chromosome/Scaffold: 016275375 Start: 11535 End: 24698 |
| Description | acyl activating enzyme 1 |
| View CDS | |
| External Links |
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| NCBI Taxonomy |
| CAZyDB |
| Signature Domain Download full data set without filtering | |||
|---|---|---|---|
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| Family | Start | End | Evalue |
| GT1 | 1016 | 1263 | 0 |
| LIWWGIRGYINDFRKKKLKLSPIAYFSTYHGSISHLPTGYMWSPHVVPKPSDWGSLVDVVGYCFLNLGSKYQPQDEFVEWIQKGPKPIYIGFGSMPLDDP KKTTNIILEALKDTGQRGIIDRGWGDLGNVTEASNNVFLLEDSPHDWLFPQCSAVVHHGGAGTTATGLRAGCPTTIVPFFGDQFFWGDRIHEKGLGPVPI PISQLSVENLSNAIRFMLEPEVKSRVLEIAKLIENEDGVAAAVDAFHR | |||
| Full Sequence |
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| Protein Sequence Length: 1302 Download |
| MEGSIRCSAN YIPLSPISFL ERSAIVYRDR PSVVYGNIVY TWRQTLERCT RLASALAQLG 60 VSYGDVVAAL APNIPAMYEL HFGVPMAGAV LCTLNIRHDS AMVSVLLKHS DAKIIFVDYQ 120 LLDVAKGAID ILSKTRTKLP VLVLIPESDQ SSPDFCNPTS GNLDYESLLG RGNFDFEIRR 180 PKDEWDAISL NYTSGTTSSP KGVIYSHRGA YLNSFAAALL NEMSSLPVYL WCVPMFHCNG 240 WCLTWAVAAQ GGTNICQRNV TAKGIFSHIS EHKVTHMGGA PTILNMIVNA PVHERRPLPG 300 KVIVMTGGAP PPAHVLFKME ELGFSVTHSY GLTETYGPGT VCAWKPEWDS LPRDEQAKIK 360 SRQGLQHLGM EEVDVKDPVT MKTVPSDAKT MGEVMFRGNT VMNGYLKDHQ ATTDAFKGGW 420 FRSGDLGVKH PDGYVELKDR LKDIIISGGE NISTIEVESV LFSHPDILEA AIVGRPDDYW 480 GETPCAFVKL KDGCNTDKEE IIKFCRNRLP HYMAPRTVVF SDLPKTSTGK VQNVRLCQPR 540 IGKILLPTII TWEVLARYSS VDRHRGGAID DYRVCKQVDS QGLPSDLGWI DAAKRKDYFT 600 NSLADVDVHQ LPLSIRAVHD LHVEQLNFDN FDFWALFLKR FELIRGAEIK VVGVGVWSVS 660 VAHPRALGKM GSNGVDHPLK HLEEEGGSSQ GQSKDFLLSC RIYDSVEMDN KYSNERLVAG 720 CFQQERNQKT NSSRPSPVLE IFQAKEFNVG SSPRRGMLIE DYEIRFPRSR MERKGRHDLK 780 LDRLSEREKR KLIVEMVKIQ NDGTVEVDLG KSAPVASEFL ELQTVEDVPI NLDDTPSNTI 840 KSIPRLKICI LVVGTRGDEF GHHVRLATHV NFSSFVKSAG VDFYPLGGDP RVLAGYMARN 900 KGLIPSGRAE ILIQRKQLKA IIESLLPACT EPDIETGVPF KAQAIVANPP AYGHAHVAEA 960 LGVPLHIFFT MPWTNPLEEF FKLTIWKHSR VSEMFPGIHN NIYSSILISY IIVDLLIWWG 1020 IRGYINDFRK KKLKLSPIAY FSTYHGSISH LPTGYMWSPH VVPKPSDWGS LVDVVGYCFL 1080 NLGSKYQPQD EFVEWIQKGP KPIYIGFGSM PLDDPKKTTN IILEALKDTG QRGIIDRGWG 1140 DLGNVTEASN NVFLLEDSPH DWLFPQCSAV VHHGGAGTTA TGLRAGCPTT IVPFFGDQFF 1200 WGDRIHEKGL GPVPIPISQL SVENLSNAIR FMLEPEVKSR VLEIAKLIEN EDGVAAAVDA 1260 FHRQLPPELP LQPASSEEDE LPSPMVLLFL QLQKWSCLPF GM |
| Functional Domains Download unfiltered results here | ||||||||
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| Cdd ID | Domain | E-Value | Start | End | Length | Domain Description | ||
| cd05915 | ttLC_FACS_like | 6.0e-171 | 13 | 532 | 529 | + Fatty acyl-CoA synthetases similar to LC-FACS from Thermus thermophiles. This family includes fatty acyl-CoA synthetases that can activate medium-chain to long-chain fatty acids. They catalyze the ATP-dependent acylation of fatty acids in a two-step reaction. The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. Fatty acyl-CoA synthetases are responsible for fatty acid degradation as well as physiological regulation of cellular functions via the production of fatty acyl-CoA esters. The fatty acyl-CoA synthetase from Thermus thermophiles in this family has been shown to catalyze the long-chain fatty acid, myristoyl acid, while another member in this family, the AlkK protein identified in Pseudomonas oleovorans, targets medium chain fatty acids. This family also includes an uncharacterized subgroup of FACS. | ||
| cd12118 | ttLC_FACS_AEE21_like | 0 | 11 | 536 | 526 | + Fatty acyl-CoA synthetases similar to LC-FACS from Thermus thermophiles and Arabidopsis. This family includes fatty acyl-CoA synthetases that can activate medium to long-chain fatty acids. These enzymes catalyze the ATP-dependent acylation of fatty acids in a two-step reaction. The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. Fatty acyl-CoA synthetases are responsible for fatty acid degradation as well as physiological regulation of cellular functions via the production of fatty acyl-CoA esters. The fatty acyl-CoA synthetase from Thermus thermophiles in this family has been shown to catalyze the long-chain fatty acid, myristoyl acid. Also included in this family are acyl activating enzymes from Arabidopsis, which contains a large number of proteins from this family with up to 63 different genes, many of which are uncharacterized. | ||
| PRK08162 | PRK08162 | 0 | 2 | 536 | 536 | + acyl-CoA synthetase; Validated | ||
| PLN02479 | PLN02479 | 0 | 6 | 532 | 543 | + acetate-CoA ligase | ||
| PLN03102 | PLN03102 | 0 | 1 | 536 | 552 | + acyl-activating enzyme; Provisional | ||
| Gene Ontology | |
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| GO Term | Description |
| GO:0003824 | catalytic activity |
| GO:0005975 | carbohydrate metabolic process |
| GO:0008152 | metabolic process |
| GO:0016758 | transferase activity, transferring hexosyl groups |
| GO:0030259 | lipid glycosylation |
| Annotations - NR Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| RefSeq | XP_002265312.1 | 0 | 661 | 1301 | 43 | 681 | PREDICTED: hypothetical protein [Vitis vinifera] |
| RefSeq | XP_002270022.1 | 0 | 1 | 536 | 10 | 548 | PREDICTED: similar to adenosine monophosphate binding protein 1 AMPBP1 [Vitis vinifera] |
| RefSeq | XP_002302172.1 | 0 | 670 | 1299 | 1 | 614 | predicted protein [Populus trichocarpa] |
| RefSeq | XP_002307787.1 | 0 | 1 | 536 | 1 | 533 | predicted protein [Populus trichocarpa] |
| RefSeq | XP_002510668.1 | 0 | 1 | 536 | 1 | 533 | AMP dependent ligase, putative [Ricinus communis] |
| Annotations - PDB Download unfiltered results here | |||||||
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| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| PDB | 1v26_B | 0 | 19 | 530 | 23 | 524 | A Chain A, Crystal Structure Of Glycogen Debranching Enzyme Trex From Sulfolobus Solfataricus |
| PDB | 1v26_A | 0 | 19 | 530 | 23 | 524 | A Chain A, Crystal Structure Of Glycogen Debranching Enzyme Trex From Sulfolobus Solfataricus |
| PDB | 1v25_B | 0 | 19 | 530 | 23 | 524 | A Chain A, Crystal Structure Of Glycogen Debranching Enzyme Trex From Sulfolobus Solfataricus |
| PDB | 1v25_A | 0 | 19 | 530 | 23 | 524 | A Chain A, Crystal Structure Of Glycogen Debranching Enzyme Trex From Sulfolobus Solfataricus |
| PDB | 1ult_B | 0 | 19 | 530 | 23 | 524 | A Chain A, Crystal Structure Of Tt0168 From Thermus Thermophilus Hb8 |
| Hydropathy |
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| EST Download unfiltered results here | ||||
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| Hit | Length | Start | End | EValue |
| HO779296 | 540 | 8 | 532 | 0 |
| ES790091 | 548 | 5 | 536 | 0 |
| HO801427 | 432 | 864 | 1290 | 0 |
| ES790056 | 542 | 1 | 538 | 0 |
| DY268391 | 323 | 159 | 481 | 0 |
| Sequence Alignments (This image is cropped. Click for full image.) |
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