| Basic Information | |
|---|---|
| Species | Malus domestica |
| Cazyme ID | MDP0000256199 |
| Family | AA7 |
| Protein Properties | Length: 472 Molecular Weight: 52875.7 Isoelectric Point: 7.6987 |
| Chromosome | Chromosome/Scaffold: 000662220 Start: 27772 End: 29662 |
| Description | cytokinin oxidase/dehydrogenase 6 |
| View CDS | |
| External Links |
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| NCBI Taxonomy |
| CAZyDB |
| Signature Domain Download full data set without filtering | |||
|---|---|---|---|
| Family | Start | End | Evalue |
| AA7 | 64 | 264 | 4.3e-21 |
| GNRYQFLPVAVLHPKTVSDIATIVKHVWDLGPRSELTVSARGHGHSLQGQSQAHRGVVINMESLKGPQIQIHTGSSPYVDASGGELWINILRESLKYGLA PKSWTDYLHLTIGGTLSNAGISGQAFRHGPQISNVHQLEVVTDELKMSAGKGEVVNCSEEQNEDLFHSVLGGLGQFGIITRARIFLEAAPARVKWIRVLY S | |||
| Full Sequence |
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| Protein Sequence Length: 472 Download |
| MRYPPIYFLK QMNMLXIEGF MVLLVSCLTI RTNLCSPNTP SLKTLPLHGH FDFHEVHHAA 60 RDFGNRYQFL PVAVLHPKTV SDIATIVKHV WDLGPRSELT VSARGHGHSL QGQSQAHRGV 120 VINMESLKGP QIQIHTGSSP YVDASGGELW INILRESLKY GLAPKSWTDY LHLTIGGTLS 180 NAGISGQAFR HGPQISNVHQ LEVVTDELKM SAGKGEVVNC SEEQNEDLFH SVLGGLGQFG 240 IITRARIFLE AAPARVKWIR VLYSDFTAFT QDQEHLMSAE NTFDYIEGFV IINRTGLLNN 300 WRSSFNSKDP VQASQFKSDG KTLFCLELAK YFNLDKXDLI TEEVDNSLSQ LSYIRSTLFV 360 SEVAYIDFLD RVHVSEIKLR SKDLWDVPHP WLNLLIPKSK IHSFAKEVFG NILTETSNGP 420 VLIYPANRSK SVTSNVSLSP KVGQQNFCCY TGGRYFLLXG IPNLCGSLVI RN 480 |
| Functional Domains Download unfiltered results here | ||||||||
|---|---|---|---|---|---|---|---|---|
| Cdd ID | Domain | E-Value | Start | End | Length | Domain Description | ||
| TIGR01679 | bact_FAD_ox | 5.0e-5 | 71 | 315 | 247 | + FAD-linked oxidoreductase. This model represents a family of bacterial oxidoreductases with covalently linked FAD, closely related to two different eukaryotic oxidases, L-gulonolactone oxidase (EC 1.1.3.8) from rat and D-arabinono-1,4-lactone oxidase (EC 1.1.3.37) from Saccharomyces cerevisiae. | ||
| COG0277 | GlcD | 3.0e-19 | 64 | 272 | 215 | + FAD/FMN-containing dehydrogenases [Energy production and conversion] | ||
| pfam01565 | FAD_binding_4 | 2.0e-21 | 71 | 221 | 152 | + FAD binding domain. This family consists of various enzymes that use FAD as a co-factor, most of the enzymes are similar to oxygen oxidoreductase. One of the enzymes Vanillyl-alcohol oxidase (VAO) has a solved structure, the alignment includes the FAD binding site, called the PP-loop, between residues 99-110. The FAD molecule is covalently bound in the known structure, however the residue that links to the FAD is not in the alignment. VAO catalyzes the oxidation of a wide variety of substrates, ranging form aromatic amines to 4-alkylphenols. Other members of this family include D-lactate dehydrogenase, this enzyme catalyzes the conversion of D-lactate to pyruvate using FAD as a co-factor; mitomycin radical oxidase, this enzyme oxidises the reduced form of mitomycins and is involved in mitomycin resistance. This family includes MurB an UDP-N-acetylenolpyruvoylglucosamine reductase enzyme EC:1.1.1.158. This enzyme is involved in the biosynthesis of peptidoglycan. | ||
| pfam09265 | Cytokin-bind | 5.0e-85 | 253 | 430 | 182 | + Cytokinin dehydrogenase 1, FAD and cytokinin binding. Members of this family adopt an alpha+beta sandwich structure with an antiparallel beta-sheet, in a ferredoxin-like fold. They are predominantly found in plant cytokinin dehydrogenase 1, where they are capable of binding both FAD and cytokinin substrates. The substrate displays a 'plug-into-socket' binding mode that seals the catalytic site and precisely positions the carbon atom undergoing oxidation in close contact with the reactive locus of the flavin. | ||
| PLN02441 | PLN02441 | 0 | 10 | 430 | 431 | + cytokinin dehydrogenase | ||
| Gene Ontology | |
|---|---|
| GO Term | Description |
| GO:0008762 | UDP-N-acetylmuramate dehydrogenase activity |
| GO:0009690 | cytokinin metabolic process |
| GO:0016491 | oxidoreductase activity |
| GO:0019139 | cytokinin dehydrogenase activity |
| GO:0050660 | flavin adenine dinucleotide binding |
| Annotations - NR Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| EMBL | CBI28611.1 | 0 | 1 | 430 | 31 | 453 | unnamed protein product [Vitis vinifera] |
| RefSeq | XP_002270841.1 | 0 | 1 | 430 | 1 | 423 | PREDICTED: hypothetical protein [Vitis vinifera] |
| RefSeq | XP_002304773.1 | 0 | 14 | 430 | 1 | 411 | cytokinin oxidase [Populus trichocarpa] |
| RefSeq | XP_002332424.1 | 0 | 1 | 430 | 1 | 424 | cytokinin oxidase [Populus trichocarpa] |
| RefSeq | XP_002513118.1 | 0 | 1 | 430 | 1 | 424 | Cytokinin dehydrogenase, putative [Ricinus communis] |
| Annotations - PDB Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| PDB | 3s1d_A | 0 | 37 | 429 | 15 | 418 | A Chain A, Glu381ser Mutant Of Maize Cytokinin OxidaseDEHYDROGENASE COMPLEXED With N6-Isopentenyladenosine |
| PDB | 3s1c_A | 0 | 37 | 429 | 15 | 418 | A Chain A, Glu381ser Mutant Of Maize Cytokinin OxidaseDEHYDROGENASE COMPLEXED With N6-Isopentenyladenosine |
| PDB | 3dq0_A | 0 | 37 | 429 | 15 | 418 | A Chain A, Glu381ser Mutant Of Maize Cytokinin OxidaseDEHYDROGENASE COMPLEXED With N6-Isopentenyladenosine |
| PDB | 3c0p_A | 0 | 37 | 429 | 15 | 418 | A Chain A, Glu381ser Mutant Of Maize Cytokinin OxidaseDEHYDROGENASE COMPLEXED With N6-Isopentenyladenosine |
| PDB | 3bw7_A | 0 | 37 | 429 | 15 | 418 | A Chain A, Glu381ser Mutant Of Maize Cytokinin OxidaseDEHYDROGENASE COMPLEXED With N6-Isopentenyladenosine |
| Hydropathy |
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| EST Download unfiltered results here | ||||
|---|---|---|---|---|
| Hit | Length | Start | End | EValue |
| JK618028 | 282 | 68 | 349 | 0 |
| DY963441 | 308 | 49 | 354 | 0 |
| ES837714 | 269 | 162 | 430 | 0 |
| EB680403 | 311 | 27 | 316 | 0 |
| FS319644 | 269 | 11 | 278 | 0 |
| Sequence Alignments (This image is cropped. Click for full image.) |
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