Basic Information | |
---|---|
Species | Malus domestica |
Cazyme ID | MDP0000267261 |
Family | GT8 |
Protein Properties | Length: 615 Molecular Weight: 69683.9 Isoelectric Point: 5.0124 |
Chromosome | Chromosome/Scaffold: 001326605 Start: 2836 End: 8652 |
Description | galactinol synthase 2 |
View CDS |
External Links |
---|
NCBI Taxonomy |
CAZyDB |
Signature Domain Download full data set without filtering | |||
---|---|---|---|
Family | Start | End | Evalue |
GT8 | 54 | 289 | 5.6e-39 |
YVTMLAGDYDEALFGXVGLAKGLKKRMSKYPLVVVVLPDVSEEYRKLLASHGCTVKEIEPVFRASYVTNYFKLRIWALEEYDKMIFLDGNVXVFENIDHM FDYPDSYFYAALDCFCENSWSHSPHHKIGHCQQWPNKVQWDPTLGPKPPLYFNASVFVFEPSLVTYSDLIKXLQTSRTTAFAEQDLLNVFFKDKLVALAP HYNLMLPMLWRHPVAVELDRVKVVNYCANGSKPWSY |
Full Sequence |
---|
Protein Sequence Length: 615 Download |
MSSANPHRDL VEFPLAGDDD DIRSQTYRRR LAIMHIDLKA QGAFSAAXEP RRAYVTMLAG 60 DYDEALFGXV GLAKGLKKRM SKYPLVVVVL PDVSEEYRKL LASHGCTVKE IEPVFRASYV 120 TNYFKLRIWA LEEYDKMIFL DGNVXVFENI DHMFDYPDSY FYAALDCFCE NSWSHSPHHK 180 IGHCQQWPNK VQWDPTLGPK PPLYFNASVF VFEPSLVTYS DLIKXLQTSR TTAFAEQDLL 240 NVFFKDKLVA LAPHYNLMLP MLWRHPVAVE LDRVKVVNYC ANGSKPWSYT GKEENMERED 300 VKMLFDQNRW PADSNDSVFV CVFWCMSFPL MLWKSVRGDV VLETKTYGCR ALVVQRFEEL 360 FSQTKSKGAA ELAAESPMGM LLTPDTVAKF QMSSADPGHR DDEEAPLAGD DEDTGAQVAP 420 IVRLEEVAVT TGEEEEDAIL DLKAKLYRFD KDGNQWKERG AGTVKFLKHK KSGKVRLVMR 480 QSKTLKICAN HLVLPKMTVQ EHAGNEKSCV WHATDFADGE LKEELFCIRF ASIENNKLFM 540 EKFQEVAESQ SPEEESKEAT EAAGLLDKLS VGEKSEAEAP AAAKKSDAXK EESPAEEKAE 600 EKAPAAKEEK KDDQE |
Functional Domains Download unfiltered results here | ||||||||
---|---|---|---|---|---|---|---|---|
Cdd ID | Domain | E-Value | Start | End | Length | Domain Description | ||
cd13176 | RanBD1_RanBD3_RanBP2_mammal-like | 2.0e-40 | 443 | 545 | 103 | + Ran-binding protein 2, Ran binding domain repeats 1 and 3. RanBP2 (also called E3 SUMO-protein ligase RanBP2, 358 kDa nucleoporin, and nuclear pore complex (NPC) protein Nup358) is a giant nucleoporin that localizes to the cytosolic face of the NPC. RanBP2 contains a leucine-rich region, 8 zinc-finger motifs, a cyclophilin A homologous domain, and 4 RanBDs. Ran is a Ras-like nuclear small GTPase, which regulates receptor-mediated transport between the nucleus and the cytoplasm. RanGTP hydrolysis is stimulated by RanGAP together with the Ran-binding domain containing acessory proteins RanBP1 and RanBP2. These accessory proteins stabilize the active GTP-bound form of Ran. All eukaryotic cells contain RanBP1, but in vertebrates however, the main RanBP seems to be RanBP2. There is no RanBP2 ortholog in yeast. Transport complex disassembly is accomplished by a small ubiquitin-related modifier-1 (SUMO-1)-modified version of RanGAP that is bound to RanBP2. RanBP1 acts as a second line of defense against exported RanGTP-importin complexes which have escaped from dissociation by RanBP2. RanBP2 also interacts with the importin subunit beta-1. RabBD shares structural similarity to the PH domain, but lacks detectable sequence similarity. The members here include human, chicken, frog, tunicates, sea urchins, ticks, sea anemones, and sponges. RanBD repeats 1 and 3 are present in this hierarchy. | ||
cd00835 | RanBD_family | 3.0e-43 | 437 | 545 | 109 | + Ran-binding domain. The RanBD is present in RanBD1, RanBD2, RanBD3, Nuc2, and Nuc50. Most of these proteins have a single RanBD, with the exception of RanBD2 which has 4 RanBDs. Ran is a Ras-like nuclear small GTPase, which regulates receptor-mediated transport between the nucleus and the cytoplasm. RanGTP hydrolysis is stimulated by RanGAP together with the Ran-binding domain containing acessory proteins RanBP1 and RanBP2. These accessory proteins stabilize the active GTP-bound form of Ran. The Ran-binding domain is found in multiple copies in Nuclear pore complex proteins. RabBD shares structural similarity to the PH domain, but lacks detectable sequence similarity. The RanBD proteins of the nuclear pore complex (NPC): nucleoporin 1 (NUP1), NUP2, NUP61, and Nuclear Pore complex Protein 9 (npp-9) are present in the parent, but specific models were not made due to lineage. To date there been no reports of inositol phosphate or phosphoinositide binding by Ran-binding proteins. | ||
cd13179 | RanBD_RanBP1 | 6.0e-46 | 437 | 545 | 112 | + Ran-binding domain. RanBP1 interacts specifically with GTP-charged Ran. RanBP1 does not activate GTPase activity of Ran, but does markedly increase GTP hydrolysis by the RanGTPase-activating protein (RanGAP1). In both mammalian cells and in yeast, RanBP1 acts as a negative regulator of Regulator of chromosome condensation 1 (RCC1) by inhibiting RCC1-stimulated guanine nucleotide release from Ran. In addition to Ran, RanBP1 has been shown to interact with Exportin-1 and Importin subunit beta-1 which docks the NPC at the cytoplasmic side of the nuclear pore complex. RabBP1 contains a single RanBD. The RanBD is present in RanBD1, RanBD2, RanBD3, Nuc2, and Nuc50. Most of these proteins have a single RanBD, with the exception of RanBD2 which has 4 RanBDs. Ran is a Ras-like nuclear small GTPase, which regulates receptor-mediated transport between the nucleus and the cytoplasm. RanGTP hydrolysis is stimulated by RanGAP together with the Ran-binding domain containing acessory proteins RanBP1 and RanBP2. These accessory proteins stabilize the active GTP-bound form of Ran. The Ran-binding domain is found in multiple copies in Nuclear pore complex proteins. RabBD shares structural similarity to the PH domain, but lacks detectable sequence similarity. | ||
cd02537 | GT8_Glycogenin | 1.0e-56 | 52 | 307 | 268 | + Glycogenin belongs the GT 8 family and initiates the biosynthesis of glycogen. Glycogenin initiates the biosynthesis of glycogen by incorporating glucose residues through a self-glucosylation reaction at a Tyr residue, and then acts as substrate for chain elongation by glycogen synthase and branching enzyme. It contains a conserved DxD motif and an N-terminal beta-alpha-beta Rossmann-like fold that are common to the nucleotide-binding domains of most glycosyltransferases. The DxD motif is essential for coordination of the catalytic divalent cation, most commonly Mn2+. Glycogenin can be classified as a retaining glycosyltransferase, based on the relative anomeric stereochemistry of the substrate and product in the reaction catalyzed. It is placed in glycosyltransferase family 8 which includes lipopolysaccharide glucose and galactose transferases and galactinol synthases. | ||
PLN00176 | PLN00176 | 5.0e-146 | 34 | 304 | 280 | + galactinol synthase |
Gene Ontology | |
---|---|
GO Term | Description |
GO:0016757 | transferase activity, transferring glycosyl groups |
GO:0046907 | intracellular transport |
Annotations - NR Download unfiltered results here | |||||||
---|---|---|---|---|---|---|---|
Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
GenBank | AAM96867.1 | 0 | 46 | 304 | 19 | 285 | galactinol synthase [Glycine max] |
GenBank | ABF66656.1 | 0 | 34 | 304 | 1 | 285 | galactinol synthase [Ammopiptanthus mongolicus] |
GenBank | ABQ44212.1 | 0 | 39 | 316 | 11 | 294 | galactinol synthase [Capsicum annuum] |
RefSeq | XP_002512546.1 | 0 | 53 | 304 | 27 | 286 | conserved hypothetical protein [Ricinus communis] |
RefSeq | XP_002512547.1 | 0 | 53 | 304 | 27 | 286 | conserved hypothetical protein [Ricinus communis] |
Annotations - PDB Download unfiltered results here | |||||||
---|---|---|---|---|---|---|---|
Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
PDB | 4gpt_B | 8e-36 | 420 | 548 | 6 | 135 | C Chain C, Crystal Structure Of Kpt251 In Complex With Crm1-ran-ranbp1 |
PDB | 3m1i_B | 2e-35 | 403 | 548 | 38 | 186 | C Chain C, Crystal Structure Of Yeast Crm1 (Xpo1p) In Complex With Yeas (Yrb1p) And Yeast Rangtp (Gsp1pgtp) |
PDB | 4hb4_B | 2e-35 | 420 | 548 | 6 | 135 | C Chain C, Crystal Structure Of Crm1 Inhibitor Leptomycin B In Complex With Crm1(537dltvk541/glceq)-ran-ran |
PDB | 4hb3_B | 2e-35 | 420 | 548 | 6 | 135 | C Chain C, Crystal Structure Of Crm1(t539s)-ran-ranbp1 With Weakly Bound Unmodeled Leptomycin B |
PDB | 4hb2_B | 2e-35 | 420 | 548 | 6 | 135 | C Chain C, Crystal Structure Of Crm1-ran-ranbp1 |
EST Download unfiltered results here | ||||
---|---|---|---|---|
Hit | Length | Start | End | EValue |
CV198253 | 276 | 38 | 304 | 0 |
CN942063 | 193 | 380 | 572 | 0 |
CN848726 | 193 | 380 | 572 | 0 |
CO902483 | 189 | 384 | 572 | 0 |
CN992910 | 192 | 380 | 571 | 0 |
Sequence Alignments (This image is cropped. Click for full image.) |
---|