| Basic Information | |
|---|---|
| Species | Malus domestica |
| Cazyme ID | MDP0000279125 |
| Family | AA7 |
| Protein Properties | Length: 582 Molecular Weight: 64772.3 Isoelectric Point: 6.3873 |
| Chromosome | Chromosome/Scaffold: 006803275 Start: 4872 End: 8728 |
| Description | cytokinin oxidase 7 |
| View CDS | |
| External Links |
|---|
| NCBI Taxonomy |
| CAZyDB |
| Signature Domain Download full data set without filtering | |||
|---|---|---|---|
 | |||
| Family | Start | End | Evalue |
| AA7 | 48 | 219 | 6.9e-27 |
| FPPLAFIRPAGADDVARVVKEAARSSNLTVAARGNGHSINGQAMADRGLVLDMRSLGEHLRVVRASDGSVYADVSGGALWEDVLKRCVSEHGMAPRSWTD YLSLTVGGTLSNAGVSGQAFRYGPQTSNVTELEVVTGKGEIFNCSEKENSELFFGALGGLGQFGIITKAKVR | |||
| Full Sequence |
|---|
| Protein Sequence Length: 582 Download |
| MIAYLECFVS ESRHDDGVSS LSKALGLQGT VADFGGEAGR DFGGMKSFPP LAFIRPAGAD 60 DVARVVKEAA RSSNLTVAAR GNGHSINGQA MADRGLVLDM RSLGEHLRVV RASDGSVYAD 120 VSGGALWEDV LKRCVSEHGM APRSWTDYLS LTVGGTLSNA GVSGQAFRYG PQTSNVTELE 180 VVTGKGEIFN CSEKENSELF FGALGGLGQF GIITKAKVRW IRLVYTEFDD FTRDAELLVT 240 RQDDGGDSFD YVEGFAFVNS DNPADGRPSV PLDPQETFDP AHLPRTSGPI LYCLELAHHY 300 RNTDRPSSVD TGVNRLLGGL GFVEELKFQV DLSYVEFLLR VKRAEEHAKA NGIWDAPHPW 360 LNMFVSKSDI ADFDRTVFKK ILKDGIGGPM LVYPLRRSKW YRLWTSFSEL DTLTKGENIL 420 NRMVVKLGQI FSTCKVIKWD PIGGLMTVVP FELCTGPVGN PDPHVXEVTI YAMWRAGWDT 480 RTSVVLPESE IFYIVALLRF TPPYPKGPSA ENLVAQNQEI IQYCTRKGFD FKLYLPHYRS 540 QEDWKRHFGS DQWSRFVERK KCFDPMAILA PGQKIFSRVP QP |
| Functional Domains Download unfiltered results here | ||||||||
|---|---|---|---|---|---|---|---|---|
 | ||||||||
| Cdd ID | Domain | E-Value | Start | End | Length | Domain Description | ||
| pfam01565 | FAD_binding_4 | 5.0e-31 | 50 | 192 | 143 | + FAD binding domain. This family consists of various enzymes that use FAD as a co-factor, most of the enzymes are similar to oxygen oxidoreductase. One of the enzymes Vanillyl-alcohol oxidase (VAO) has a solved structure, the alignment includes the FAD binding site, called the PP-loop, between residues 99-110. The FAD molecule is covalently bound in the known structure, however the residue that links to the FAD is not in the alignment. VAO catalyzes the oxidation of a wide variety of substrates, ranging form aromatic amines to 4-alkylphenols. Other members of this family include D-lactate dehydrogenase, this enzyme catalyzes the conversion of D-lactate to pyruvate using FAD as a co-factor; mitomycin radical oxidase, this enzyme oxidises the reduced form of mitomycins and is involved in mitomycin resistance. This family includes MurB an UDP-N-acetylenolpyruvoylglucosamine reductase enzyme EC:1.1.1.158. This enzyme is involved in the biosynthesis of peptidoglycan. | ||
| pfam09265 | Cytokin-bind | 3.0e-42 | 470 | 576 | 107 | + Cytokinin dehydrogenase 1, FAD and cytokinin binding. Members of this family adopt an alpha+beta sandwich structure with an antiparallel beta-sheet, in a ferredoxin-like fold. They are predominantly found in plant cytokinin dehydrogenase 1, where they are capable of binding both FAD and cytokinin substrates. The substrate displays a 'plug-into-socket' binding mode that seals the catalytic site and precisely positions the carbon atom undergoing oxidation in close contact with the reactive locus of the flavin. | ||
| PLN02441 | PLN02441 | 3.0e-49 | 470 | 582 | 113 | + cytokinin dehydrogenase | ||
| pfam09265 | Cytokin-bind | 6.0e-70 | 216 | 400 | 186 | + Cytokinin dehydrogenase 1, FAD and cytokinin binding. Members of this family adopt an alpha+beta sandwich structure with an antiparallel beta-sheet, in a ferredoxin-like fold. They are predominantly found in plant cytokinin dehydrogenase 1, where they are capable of binding both FAD and cytokinin substrates. The substrate displays a 'plug-into-socket' binding mode that seals the catalytic site and precisely positions the carbon atom undergoing oxidation in close contact with the reactive locus of the flavin. | ||
| PLN02441 | PLN02441 | 0 | 1 | 400 | 417 | + cytokinin dehydrogenase | ||
| Gene Ontology | |
|---|---|
| GO Term | Description |
| GO:0008762 | UDP-N-acetylmuramate dehydrogenase activity |
| GO:0009690 | cytokinin metabolic process |
| GO:0016491 | oxidoreductase activity |
| GO:0019139 | cytokinin dehydrogenase activity |
| GO:0050660 | flavin adenine dinucleotide binding |
| Annotations - NR Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| RefSeq | XP_002279960.1 | 0 | 10 | 441 | 15 | 436 | PREDICTED: hypothetical protein [Vitis vinifera] |
| RefSeq | XP_002279960.1 | 5.04467e-44 | 470 | 582 | 404 | 515 | PREDICTED: hypothetical protein [Vitis vinifera] |
| RefSeq | XP_002309468.1 | 0 | 1 | 400 | 1 | 417 | cytokinin oxidase [Populus trichocarpa] |
| RefSeq | XP_002309468.1 | 1.4013e-45 | 468 | 581 | 407 | 520 | cytokinin oxidase [Populus trichocarpa] |
| RefSeq | XP_002516133.1 | 0 | 1 | 400 | 1 | 416 | gulonolactone oxidase, putative [Ricinus communis] |
| Annotations - PDB Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| PDB | 2q4w_A | 0 | 2 | 400 | 2 | 422 | A Chain A, Structure Of The Thermostable Pectate Lyase Pl 47 |
| PDB | 2q4w_A | 6.00036e-42 | 442 | 578 | 392 | 522 | A Chain A, Structure Of The Thermostable Pectate Lyase Pl 47 |
| PDB | 2exr_A | 0 | 2 | 400 | 2 | 422 | A Chain A, X-Ray Structure Of Cytokinin OxidaseDEHYDROGENASE (CKX) FROM Arabidopsis Thaliana At5g21482 |
| PDB | 2exr_A | 6.00036e-42 | 442 | 578 | 392 | 522 | A Chain A, X-Ray Structure Of Cytokinin OxidaseDEHYDROGENASE (CKX) FROM Arabidopsis Thaliana At5g21482 |
| PDB | 3s1d_A | 0 | 24 | 400 | 22 | 420 | A Chain A, Glu381ser Mutant Of Maize Cytokinin OxidaseDEHYDROGENASE COMPLEXED With N6-Isopentenyladenosine |
| Hydropathy |
|---|
 |
| EST Download unfiltered results here | ||||
|---|---|---|---|---|
 | ||||
| Hit | Length | Start | End | EValue |
| HO781924 | 413 | 1 | 400 | 0 |
| ES791244 | 330 | 79 | 399 | 0 |
| FC070877 | 298 | 24 | 310 | 0 |
| GO520859 | 201 | 166 | 357 | 0 |
| HO781924 | 109 | 468 | 576 | 2e-36 |
| Sequence Alignments (This image is cropped. Click for full image.) |
|---|
 |
