| Basic Information | |
|---|---|
| Species | Malus domestica |
| Cazyme ID | MDP0000292695 |
| Family | GH16 |
| Protein Properties | Length: 355 Molecular Weight: 40760.8 Isoelectric Point: 8.4141 |
| Chromosome | Chromosome/Scaffold: 013462165 Start: 13009 End: 16891 |
| Description | xyloglucan endotransglucosylase/hydrolase 30 |
| View CDS | |
| External Links |
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| NCBI Taxonomy |
| CAZyDB |
| Signature Domain Download full data set without filtering | |||
|---|---|---|---|
| Family | Start | End | Evalue |
| GH16 | 55 | 229 | 1.9e-26 |
| GDGNLVRSPDGKGVRLLLDRFTGSGFISSNLYNHGFFSANIKLPSDYTAGICVAFYTSNADVFEKNHDELDIEFLGNTEGKPWRFQTNLYGNGSTNRGRE ERYRLWFDPTKDFHRYSILWTPENTIFYVDEVPIREVVRNEEMGADYPSKPMSLYTTIWDASSWATSGGQYKVNY | |||
| Full Sequence |
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| Protein Sequence Length: 355 Download |
| MEGLQPCRYW SNGVSLFFQP LRLIPLILVC VGNVDAAFYN LTTIPFDQGY TPLFGDGNLV 60 RSPDGKGVRL LLDRFTGSGF ISSNLYNHGF FSANIKLPSD YTAGICVAFY TSNADVFEKN 120 HDELDIEFLG NTEGKPWRFQ TNLYGNGSTN RGREERYRLW FDPTKDFHRY SILWTPENTI 180 FYVDEVPIRE VVRNEEMGAD YPSKPMSLYT TIWDASSWAT SGGQYKVNYK YAPFVAEFKD 240 LVLEGCPADP IQQIPAAEAC AEHYTHLATQ DYSVITRQRR AAMRRFRQRY MYYSYCYDSL 300 RYPVPPSECV TVSAEKARFK DTGRLKFGGS HKRQSKRRSR NRNRAVPNSD NASDV 360 |
| Functional Domains Download unfiltered results here | ||||||||
|---|---|---|---|---|---|---|---|---|
| Cdd ID | Domain | E-Value | Start | End | Length | Domain Description | ||
| cd02183 | GH16_fungal_CRH1_transglycosylase | 8.0e-21 | 64 | 241 | 190 | + glycosylphosphatidylinositol-glucanosyltransferase. Group of fungal GH16 members related to Saccharomyces cerevisiae Crh1p. Chr1p and Crh2p are transglycosylases that are required for the linkage of chitin to beta(1-3)glucose branches of beta(1-6)glucan, an important step in the assembly of new cell wall. Both have been shown to be glycosylphosphatidylinositol (GPI)-anchored. A third homologous protein, Crr1p, functions in the formation of the spore wall. They belongs to the family 16 of glycosyl hydrolases that includes lichenase, xyloglucan endotransglycosylase (XET), beta-agarase, kappa-carrageenase, endo-beta-1,3-glucanase, endo-beta-1,3-1,4-glucanase, and endo-beta-galactosidase, all of which have a conserved jelly roll fold with a deep active site channel harboring the catalytic residues. | ||
| cd00413 | Glyco_hydrolase_16 | 2.0e-26 | 64 | 223 | 170 | + glycosyl hydrolase family 16. The O-Glycosyl hydrolases are a widespread group of enzymes that hydrolyse the glycosidic bond between two or more carbohydrates, or between a carbohydrate and a non-carbohydrate moiety. A glycosyl hydrolase classification system based on sequence similarity has led to the definition of more than 95 different families inlcuding glycosyl hydrolase family 16. Family 16 includes lichenase, xyloglucan endotransglycosylase (XET), beta-agarase, kappa-carrageenase, endo-beta-1,3-glucanase, endo-beta-1,3-1,4-glucanase, and endo-beta-galactosidase, all of which have a conserved jelly roll fold with a deep active site channel harboring the catalytic residues. | ||
| PLN03161 | PLN03161 | 4.0e-47 | 64 | 309 | 252 | + Probable xyloglucan endotransglucosylase/hydrolase protein; Provisional | ||
| pfam00722 | Glyco_hydro_16 | 1.0e-66 | 46 | 231 | 186 | + Glycosyl hydrolases family 16. | ||
| cd02176 | GH16_XET | 4.0e-123 | 42 | 309 | 269 | + Xyloglucan endotransglycosylase, member of glycosyl hydrolase family 16. Xyloglucan endotransglycosylases (XETs) cleave and religate xyloglucan polymers in plant cell walls via a transglycosylation mechanism. Xyloglucan is a soluble hemicellulose with a backbone of beta-1,4-linked glucose units, partially substituted with alpha-1,6-linked xylopyranose branches. It binds noncovalently to cellulose, cross-linking the adjacent cellulose microfibrils, giving it a key structural role as a matrix polymer. Therefore, XET plays an important role in all plant processes that require cell wall remodeling. | ||
| Gene Ontology | |
|---|---|
| GO Term | Description |
| GO:0004553 | hydrolase activity, hydrolyzing O-glycosyl compounds |
| GO:0005618 | cell wall |
| GO:0005975 | carbohydrate metabolic process |
| GO:0006073 | cellular glucan metabolic process |
| GO:0016762 | xyloglucan:xyloglucosyl transferase activity |
| Annotations - NR Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| GenBank | ABM91070.1 | 0 | 37 | 332 | 28 | 323 | xyloglucan endotransglycosylase/hydrolase precursor XTH-3 [Populus tremula x Populus tremuloides] |
| GenBank | ACU21372.1 | 0 | 34 | 341 | 30 | 336 | unknown [Glycine max] |
| EMBL | CBI33819.1 | 0 | 36 | 354 | 24 | 336 | unnamed protein product [Vitis vinifera] |
| RefSeq | XP_002326748.1 | 0 | 34 | 332 | 3 | 300 | predicted protein [Populus trichocarpa] |
| RefSeq | XP_002518990.1 | 0 | 34 | 332 | 29 | 327 | Xyloglucan endotransglucosylase/hydrolase protein 2 precursor, putative [Ricinus communis] |
| Annotations - PDB Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| PDB | 2vh9_B | 0 | 12 | 309 | 4 | 290 | A Chain A, Crystal Structure Of Nxg1-Deltayniig In Complex With Xllg, A Xyloglucan Derived Oligosaccharide |
| PDB | 2vh9_A | 0 | 12 | 309 | 4 | 290 | A Chain A, Crystal Structure Of Nxg1-Deltayniig In Complex With Xllg, A Xyloglucan Derived Oligosaccharide |
| PDB | 2uwc_B | 0 | 40 | 309 | 13 | 271 | A Chain A, Crystal Structure Of Nasturtium Xyloglucan Hydrolase Isoform Nxg2 |
| PDB | 2uwc_A | 0 | 40 | 309 | 13 | 271 | A Chain A, Crystal Structure Of Nasturtium Xyloglucan Hydrolase Isoform Nxg2 |
| PDB | 2uwa_C | 0 | 40 | 309 | 16 | 274 | A Chain A, Crystal Structure Of The Nasturtium Seedling Xyloglucanase Isoform Nxg1 |
| Hydropathy |
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| EST Download unfiltered results here | ||||
|---|---|---|---|---|
| Hit | Length | Start | End | EValue |
| HO778510 | 298 | 34 | 331 | 0 |
| EC962891 | 296 | 36 | 331 | 0 |
| DY674177 | 238 | 39 | 276 | 0 |
| EX530554 | 276 | 39 | 314 | 0 |
| EG667085 | 243 | 34 | 276 | 0 |
| Sequence Alignments (This image is cropped. Click for full image.) |
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