| Basic Information | |
|---|---|
| Species | Malus domestica |
| Cazyme ID | MDP0000312902 |
| Family | AA7 |
| Protein Properties | Length: 313 Molecular Weight: 34160.5 Isoelectric Point: 5.8414 |
| Chromosome | Chromosome/Scaffold: 020918109 Start: 5465 End: 8015 |
| Description | cytokinin oxidase 4 |
| View CDS | |
| External Links |
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| NCBI Taxonomy |
| CAZyDB |
| Signature Domain Download full data set without filtering | |||
|---|---|---|---|
| Family | Start | End | Evalue |
| AA7 | 38 | 232 | 3.2e-24 |
| HIVYQYASAVLNPASPNDIASLILSANNNSAPFSVAARGQGHSVRGQALTSDGVVINMTALISNGSRIVVFESPSLGSYADVGGEQRWIDVLRATLDRGL TPVSWTDYLYLSVGGTLSNAGISGQTFRYGPQINNVYELDVVTGKGELVTCSSNKVPGLFYAVLGGLGQFGIITRARIPLEPXPNRAKWVRMMYR | |||
| Full Sequence |
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| Protein Sequence Length: 313 Download |
| MVLFTTGTSV LLPALGDISG FLRNDTESIN STSTDYGHIV YQYASAVLNP ASPNDIASLI 60 LSANNNSAPF SVAARGQGHS VRGQALTSDG VVINMTALIS NGSRIVVFES PSLGSYADVG 120 GEQRWIDVLR ATLDRGLTPV SWTDYLYLSV GGTLSNAGIS GQTFRYGPQI NNVYELDVVT 180 GKGELVTCSS NKVPGLFYAV LGGLGQFGII TRARIPLEPX PNRAKWVRMM YRNFSAFSKD 240 QERLMANNGF DYLEGFLLMK QGPLDLSFYP LLDQPRITAL INQYGIIYYI EAAKYYGDST 300 RPTVDKVVEK WVL 360 |
| Functional Domains Download unfiltered results here | ||||||||
|---|---|---|---|---|---|---|---|---|
| Cdd ID | Domain | E-Value | Start | End | Length | Domain Description | ||
| TIGR01679 | bact_FAD_ox | 1.0e-7 | 43 | 252 | 214 | + FAD-linked oxidoreductase. This model represents a family of bacterial oxidoreductases with covalently linked FAD, closely related to two different eukaryotic oxidases, L-gulonolactone oxidase (EC 1.1.3.8) from rat and D-arabinono-1,4-lactone oxidase (EC 1.1.3.37) from Saccharomyces cerevisiae. | ||
| COG0277 | GlcD | 2.0e-23 | 14 | 225 | 219 | + FAD/FMN-containing dehydrogenases [Energy production and conversion] | ||
| pfam01565 | FAD_binding_4 | 5.0e-24 | 44 | 189 | 147 | + FAD binding domain. This family consists of various enzymes that use FAD as a co-factor, most of the enzymes are similar to oxygen oxidoreductase. One of the enzymes Vanillyl-alcohol oxidase (VAO) has a solved structure, the alignment includes the FAD binding site, called the PP-loop, between residues 99-110. The FAD molecule is covalently bound in the known structure, however the residue that links to the FAD is not in the alignment. VAO catalyzes the oxidation of a wide variety of substrates, ranging form aromatic amines to 4-alkylphenols. Other members of this family include D-lactate dehydrogenase, this enzyme catalyzes the conversion of D-lactate to pyruvate using FAD as a co-factor; mitomycin radical oxidase, this enzyme oxidises the reduced form of mitomycins and is involved in mitomycin resistance. This family includes MurB an UDP-N-acetylenolpyruvoylglucosamine reductase enzyme EC:1.1.1.158. This enzyme is involved in the biosynthesis of peptidoglycan. | ||
| pfam09265 | Cytokin-bind | 2.0e-25 | 221 | 309 | 96 | + Cytokinin dehydrogenase 1, FAD and cytokinin binding. Members of this family adopt an alpha+beta sandwich structure with an antiparallel beta-sheet, in a ferredoxin-like fold. They are predominantly found in plant cytokinin dehydrogenase 1, where they are capable of binding both FAD and cytokinin substrates. The substrate displays a 'plug-into-socket' binding mode that seals the catalytic site and precisely positions the carbon atom undergoing oxidation in close contact with the reactive locus of the flavin. | ||
| PLN02441 | PLN02441 | 5.0e-159 | 4 | 309 | 313 | + cytokinin dehydrogenase | ||
| Gene Ontology | |
|---|---|
| GO Term | Description |
| GO:0008762 | UDP-N-acetylmuramate dehydrogenase activity |
| GO:0009690 | cytokinin metabolic process |
| GO:0016491 | oxidoreductase activity |
| GO:0019139 | cytokinin dehydrogenase activity |
| GO:0050660 | flavin adenine dinucleotide binding |
| Annotations - NR Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| EMBL | CAN77185.1 | 0 | 17 | 310 | 41 | 339 | hypothetical protein [Vitis vinifera] |
| RefSeq | XP_002263646.1 | 0 | 17 | 309 | 41 | 338 | PREDICTED: hypothetical protein [Vitis vinifera] |
| RefSeq | XP_002263754.1 | 0 | 17 | 310 | 41 | 339 | PREDICTED: hypothetical protein [Vitis vinifera] |
| RefSeq | XP_002308300.1 | 0 | 17 | 310 | 42 | 346 | cytokinin oxidase [Populus trichocarpa] |
| RefSeq | XP_002514119.1 | 0 | 17 | 304 | 42 | 342 | gulonolactone oxidase, putative [Ricinus communis] |
| Annotations - PDB Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| PDB | 3kjm_A | 0 | 13 | 296 | 6 | 318 | B Chain B, Structural Basis Of Gibberellin(Ga3)-Induced Della Recognition By The Gibberellin Receptor |
| PDB | 2qpm_A | 0 | 13 | 296 | 6 | 318 | A Chain A, Leu492ala Mutant Of Maize Cytokinin OxidaseDEHYDROGENASE COMPLEXED With Benzylurea Inhibitor Cpbu |
| PDB | 3s1e_A | 0 | 13 | 296 | 6 | 318 | A Chain A, Pro427gln Mutant Of Maize Cytokinin OxidaseDEHYDROGENASE COMPLEXED With N6-Isopentenyladenine |
| PDB | 3s1d_A | 0 | 13 | 296 | 6 | 318 | A Chain A, Glu381ser Mutant Of Maize Cytokinin OxidaseDEHYDROGENASE COMPLEXED With N6-Isopentenyladenosine |
| PDB | 3s1c_A | 0 | 13 | 296 | 6 | 318 | A Chain A, Glu381ser Mutant Of Maize Cytokinin OxidaseDEHYDROGENASE COMPLEXED With N6-Isopentenyladenosine |
| Hydropathy |
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| EST Download unfiltered results here | ||||
|---|---|---|---|---|
| Hit | Length | Start | End | EValue |
| GT684991 | 232 | 65 | 288 | 0 |
| JG470904 | 221 | 22 | 242 | 0 |
| EH041997 | 257 | 62 | 308 | 0 |
| CO117912 | 238 | 17 | 249 | 0 |
| GR193656 | 245 | 25 | 261 | 0 |
| Sequence Alignments (This image is cropped. Click for full image.) |
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