| Basic Information | |
|---|---|
| Species | Malus domestica |
| Cazyme ID | MDP0000316265 |
| Family | AA7 |
| Protein Properties | Length: 772 Molecular Weight: 85485.6 Isoelectric Point: 5.8729 |
| Chromosome | Chromosome/Scaffold: 03269513 Start: 708 End: 10398 |
| Description | FAD-binding Berberine family protein |
| View CDS | |
| External Links |
|---|
| NCBI Taxonomy |
| CAZyDB |
| Signature Domain Download full data set without filtering | |||
|---|---|---|---|
| Family | Start | End | Evalue |
| AA7 | 275 | 758 | 0 |
| PKPVAIILPESVEQLVNSVSCSRQGLLEIRVRSGGHSYEGTSSVAVDGALFVIIDMMNLNGVSVDLETETAWVEGGATLGETYHAIAQASNLHGFSAGSC PTVGAGGHIAGGGFGLMSRKYGLAADNVVDALLVDANGQLLDRQGMGEDVFWAIRGGGGGVFGIVYAWKIQLLNVPQVVTAFVVSRAASAAAGGGGNEIF NHNVARLVDKWQHIAPYLEDDFYISCFVGAGLPEVKTTXVTATSSTNISATFKGFYLGPXSSAMSILNKVFPDLGVAEEDCAEMSWIESIVFFSGLSKGS SVSDLRNRYLQGKGFFKAKSDYVRTPISYRGIRAALEILEEEPKGYVILDPYGGVMHDISSEAIAFPHRKGNLFTIQYLVEWKEEDNHKINDYIEWIRRF HNAMTEYVSWGPRAAYINYVDLDLGVMMRQQFPLNNNLVKDDESDAVEIARNWGEKYFLNNYDRLVRAKTLIDPTNVFRNQQGI | |||
| Full Sequence |
|---|
| Protein Sequence Length: 772 Download |
| MQHKVMKEAV ENHMPVVVIV DEIGTESEAL ACRLIAERGV MLIATAHGQK LENIIKNSTL 60 TYLQSRLFTV QPLATGYRAS LLHCTLMQNV EPLREFSYEH SVNMEDLIKL RDVMVEHALG 120 SNGGDNLEKL DGRNNGYQRN KKSMLKGVML EHSLLCCFTY SDSQFAFHSN VDVERFYEFS 180 YKHAVNMKDL IKLSNVMIKH SIGPNQDRKE CRSFAAGKNP TGRAEFPADQ ITFCLTLHDI 240 NNFTTFPNTE NESAAYYKLL NFSIQNLRFA DPALPKPVAI ILPESVEQLV NSVSCSRQGL 300 LEIRVRSGGH SYEGTSSVAV DGALFVIIDM MNLNGVSVDL ETETAWVEGG ATLGETYHAI 360 AQASNLHGFS AGSCPTVGAG GHIAGGGFGL MSRKYGLAAD NVVDALLVDA NGQLLDRQGM 420 GEDVFWAIRG GGGGVFGIVY AWKIQLLNVP QVVTAFVVSR AASAAAGGGG NEIFNHNVAR 480 LVDKWQHIAP YLEDDFYISC FVGAGLPEVK TTXVTATSST NISATFKGFY LGPXSSAMSI 540 LNKVFPDLGV AEEDCAEMSW IESIVFFSGL SKGSSVSDLR NRYLQGKGFF KAKSDYVRTP 600 ISYRGIRAAL EILEEEPKGY VILDPYGGVM HDISSEAIAF PHRKGNLFTI QYLVEWKEED 660 NHKINDYIEW IRRFHNAMTE YVSWGPRAAY INYVDLDLGV MMRQQFPLNN NLVKDDESDA 720 VEIARNWGEK YFLNNYDRLV RAKTLIDPTN VFRNQQGIPP MSSSSSLVVL DH 780 |
| Functional Domains Download unfiltered results here | ||||||||
|---|---|---|---|---|---|---|---|---|
| Cdd ID | Domain | E-Value | Start | End | Length | Domain Description | ||
| TIGR02858 | spore_III_AA | 7.0e-5 | 6 | 56 | 51 | + stage III sporulation protein AA. Members of this protein are the stage III sporulation protein AA, encoded by one of several genes in the spoIIIA locus. It seems that this protein is found in a species if and only if that species is capable of endospore formation [Cellular processes, Sporulation and germination]. | ||
| pfam08031 | BBE | 4.0e-15 | 689 | 759 | 71 | + Berberine and berberine like. This domain is found in the berberine bridge and berberine bridge- like enzymes which are involved in the biosynthesis of numerous isoquinoline alkaloids. They catalyze the transformation of the N-methyl group of (S)-reticuline into the C-8 berberine bridge carbon of (S)-scoulerine. | ||
| COG3854 | SpoIIIAA | 6.0e-18 | 2 | 63 | 62 | + ncharacterized protein conserved in bacteria [Function unknown] | ||
| COG0277 | GlcD | 2.0e-18 | 277 | 760 | 496 | + FAD/FMN-containing dehydrogenases [Energy production and conversion] | ||
| pfam01565 | FAD_binding_4 | 3.0e-24 | 277 | 416 | 141 | + FAD binding domain. This family consists of various enzymes that use FAD as a co-factor, most of the enzymes are similar to oxygen oxidoreductase. One of the enzymes Vanillyl-alcohol oxidase (VAO) has a solved structure, the alignment includes the FAD binding site, called the PP-loop, between residues 99-110. The FAD molecule is covalently bound in the known structure, however the residue that links to the FAD is not in the alignment. VAO catalyzes the oxidation of a wide variety of substrates, ranging form aromatic amines to 4-alkylphenols. Other members of this family include D-lactate dehydrogenase, this enzyme catalyzes the conversion of D-lactate to pyruvate using FAD as a co-factor; mitomycin radical oxidase, this enzyme oxidises the reduced form of mitomycins and is involved in mitomycin resistance. This family includes MurB an UDP-N-acetylenolpyruvoylglucosamine reductase enzyme EC:1.1.1.158. This enzyme is involved in the biosynthesis of peptidoglycan. | ||
| Gene Ontology | |
|---|---|
| GO Term | Description |
| GO:0008762 | UDP-N-acetylmuramate dehydrogenase activity |
| GO:0016491 | oxidoreductase activity |
| GO:0050660 | flavin adenine dinucleotide binding |
| GO:0055114 | oxidation-reduction process |
| Annotations - NR Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| DDBJ | BAC65012.1 | 0 | 234 | 763 | 34 | 539 | putative berberine bridge enzyme [Oryza sativa Japonica Group] |
| EMBL | CAO99130.1 | 0 | 234 | 765 | 33 | 560 | berberine bridge enzyme like protein [Nicotiana tabacum] |
| RefSeq | XP_002264336.1 | 0 | 227 | 764 | 27 | 537 | PREDICTED: hypothetical protein [Vitis vinifera] |
| RefSeq | XP_002329230.1 | 0 | 227 | 765 | 13 | 530 | predicted protein [Populus trichocarpa] |
| RefSeq | XP_002533924.1 | 0 | 228 | 765 | 28 | 539 | d-lactate dehydrogenase, putative [Ricinus communis] |
| Annotations - PDB Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| PDB | 3fw9_A | 0 | 234 | 762 | 5 | 495 | A Chain A, Structure Of Berberine Bridge Enzyme In Complex With (S)-Scoulerine |
| PDB | 4ec3_A | 0 | 234 | 763 | 11 | 502 | A Chain A, Structure Of Berberine Bridge Enzyme, H174a Variant In Complex With (S)-Reticuline |
| PDB | 3gsy_A | 0 | 234 | 763 | 11 | 502 | A Chain A, Structure Of Berberine Bridge Enzyme In Complex With Dehydroscoulerine |
| PDB | 3d2j_A | 0 | 234 | 763 | 30 | 521 | A Chain A, Structure Of Berberine Bridge Enzyme In Complex With Dehydroscoulerine |
| PDB | 3d2h_A | 0 | 234 | 763 | 30 | 521 | A Chain A, Structure Of Berberine Bridge Enzyme In Complex With Dehydroscoulerine |
| Hydropathy |
|---|
 |
| EST Download unfiltered results here | ||||
|---|---|---|---|---|
| Hit | Length | Start | End | EValue |
| EL451713 | 317 | 445 | 761 | 0 |
| HO777438 | 548 | 220 | 759 | 0 |
| GO028240 | 285 | 477 | 761 | 0 |
| FC883009 | 247 | 256 | 502 | 0 |
| DY275245 | 255 | 256 | 510 | 0 |
| Sequence Alignments (This image is cropped. Click for full image.) |
|---|
 |
