Basic Information | |
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Species | Malus domestica |
Cazyme ID | MDP0000435532 |
Family | GT8 |
Protein Properties | Length: 579 Molecular Weight: 67374.9 Isoelectric Point: 8.8822 |
Chromosome | Chromosome/Scaffold: 021878318 Start: 3596 End: 10198 |
Description | plant glycogenin-like starch initiation protein 3 |
View CDS |
External Links |
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NCBI Taxonomy |
CAZyDB |
Signature Domain Download full data set without filtering | |||
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Family | Start | End | Evalue |
GT8 | 281 | 496 | 5.19999e-40 |
KPKREAYVTVLHSSDSYVCGAITLAKSLLQTGTKRDLVLLLDNSISAPKRDALSDAGWKLHVIERIRNPRXKKGTYNEYNYSKFRLWQLIEYDKIXFIDA DIIVLRNLDLLFHFPQMSAISNSGYLFNSGIMVIEPSNCTFKFLMDHRDDIVSNNGGDQGYLNEVFVWWHRVLKKVNAMTYFPANRTNNTTIQDKQFGAD PPKIFAIHYLGWKPWL |
Full Sequence |
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Protein Sequence Length: 579 Download |
MHIASKRNIG HLDEHGIYTG QXSTFTLCGY VKAQGEADSG LDRLWRKKKT DVDNGVKIMM 60 KSVLEETKGN IPRXKGSMSK IEVPSFLNGT MGKGMMKFAM VNMDEYDLSE WDKLGKTIPI 120 HFNHIXDNIK WDDLFPTWID EEEESDVPTC PKIPMLNIQI YEKMDMVVAK LPCKYPEEGW 180 RREVFRLQVH LIVANLAVRR GKRDGNRKTT KMLFWSKCRP MLEIFRCDDL VRQEGNWWLF 240 EPDMARLRQK VSLPXGTCNL ALPFWGQQVY DLAKIQGTTN KPKREAYVTV LHSSDSYVCG 300 AITLAKSLLQ TGTKRDLVLL LDNSISAPKR DALSDAGWKL HVIERIRNPR XKKGTYNEYN 360 YSKFRLWQLI EYDKIXFIDA DIIVLRNLDL LFHFPQMSAI SNSGYLFNSG IMVIEPSNCT 420 FKFLMDHRDD IVSNNGGDQG YLNEVFVWWH RVLKKVNAMT YFPANRTNNT TIQDKQFGAD 480 PPKIFAIHYL GWKPWLCYRD YDCNWDTKGR HVFASDVAHE RWWRVHDTVE EGLQRFCGLT 540 KQRKDYLDST RSEARKLGFP DEHWKINITD PRQHHLVA* |
Functional Domains Download unfiltered results here | ||||||||
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Cdd ID | Domain | E-Value | Start | End | Length | Domain Description | ||
pfam01249 | Ribosomal_S21e | 1.0e-12 | 8 | 49 | 42 | + Ribosomal protein S21e. | ||
PLN00176 | PLN00176 | 4.0e-15 | 272 | 426 | 198 | + galactinol synthase | ||
cd06914 | GT8_GNT1 | 6.0e-16 | 286 | 446 | 172 | + GNT1 is a fungal enzyme that belongs to the GT 8 family. N-acetylglucosaminyltransferase is a fungal enzyme that catalyzes the addition of N-acetyl-D-glucosamine to mannotetraose side chains by an alpha 1-2 linkage during the synthesis of mannan. The N-acetyl-D-glucosamine moiety in mannan plays a role in the attachment of mannan to asparagine residues in proteins. The mannotetraose and its N-acetyl-D-glucosamine derivative side chains of mannan are the principle immunochemical determinants on the cell surface. N-acetylglucosaminyltransferase is a member of glycosyltransferase family 8, which are, based on the relative anomeric stereochemistry of the substrate and product in the reaction catalyzed, retaining glycosyltransferases. | ||
pfam01501 | Glyco_transf_8 | 2.0e-36 | 288 | 498 | 253 | + Glycosyl transferase family 8. This family includes enzymes that transfer sugar residues to donor molecules. Members of this family are involved in lipopolysaccharide biosynthesis and glycogen synthesis. This family includes Lipopolysaccharide galactosyltransferase, lipopolysaccharide glucosyltransferase 1, and glycogenin glucosyltransferase. | ||
cd02537 | GT8_Glycogenin | 2.0e-63 | 285 | 527 | 254 | + Glycogenin belongs the GT 8 family and initiates the biosynthesis of glycogen. Glycogenin initiates the biosynthesis of glycogen by incorporating glucose residues through a self-glucosylation reaction at a Tyr residue, and then acts as substrate for chain elongation by glycogen synthase and branching enzyme. It contains a conserved DxD motif and an N-terminal beta-alpha-beta Rossmann-like fold that are common to the nucleotide-binding domains of most glycosyltransferases. The DxD motif is essential for coordination of the catalytic divalent cation, most commonly Mn2+. Glycogenin can be classified as a retaining glycosyltransferase, based on the relative anomeric stereochemistry of the substrate and product in the reaction catalyzed. It is placed in glycosyltransferase family 8 which includes lipopolysaccharide glucose and galactose transferases and galactinol synthases. |
Gene Ontology | |
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GO Term | Description |
GO:0003735 | structural constituent of ribosome |
GO:0005622 | intracellular |
GO:0005840 | ribosome |
GO:0006412 | translation |
GO:0016757 | transferase activity, transferring glycosyl groups |
Annotations - NR Download unfiltered results here | |||||||
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Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
GenBank | ABK25357.1 | 0 | 85 | 574 | 126 | 610 | unknown [Picea sitchensis] |
RefSeq | NP_001154284.1 | 0 | 52 | 573 | 63 | 589 | transferase, transferring glycosyl groups [Arabidopsis thaliana] |
RefSeq | NP_195059.3 | 0 | 52 | 573 | 63 | 589 | transferase, transferring glycosyl groups [Arabidopsis thaliana] |
RefSeq | XP_002282762.1 | 0 | 46 | 573 | 62 | 588 | PREDICTED: hypothetical protein [Vitis vinifera] |
RefSeq | XP_002510223.1 | 0 | 52 | 577 | 66 | 589 | glycogenin, putative [Ricinus communis] |
Annotations - PDB Download unfiltered results here | |||||||
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Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
PDB | 1zcv_A | 3e-21 | 285 | 546 | 24 | 293 | A Chain A, Apo Form Of A Mutant Of Glycogenin In Which Asp159 Is Replaced By Asn |
PDB | 1ll3_A | 9e-21 | 285 | 546 | 4 | 273 | A Chain A, Apo Form Of A Mutant Of Glycogenin In Which Asp159 Is Replaced By Asn |
PDB | 1ll2_A | 9e-21 | 285 | 546 | 4 | 273 | A Chain A, Crystal Structure Of Rabbit Muscle Glycogenin Complexed With Udp- Glucose And Manganese |
PDB | 1ll0_J | 1e-20 | 285 | 546 | 10 | 279 | A Chain A, Crystal Structure Of Rabbit Muscle Glycogenin |
PDB | 1ll0_I | 1e-20 | 285 | 546 | 10 | 279 | A Chain A, Crystal Structure Of Rabbit Muscle Glycogenin |
EST Download unfiltered results here | ||||
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Hit | Length | Start | End | EValue |
EB152076 | 237 | 268 | 504 | 0 |
EE593179 | 315 | 232 | 543 | 0 |
EB152259 | 234 | 268 | 501 | 0 |
CO084566 | 275 | 256 | 527 | 0 |
AJ778414 | 243 | 274 | 516 | 0 |
Sequence Alignments (This image is cropped. Click for full image.) |
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