| Basic Information | |
|---|---|
| Species | Malus domestica |
| Cazyme ID | MDP0000832632 |
| Family | GH5 |
| Protein Properties | Length: 430 Molecular Weight: 48134.3 Isoelectric Point: 8.291 |
| Chromosome | Chromosome/Scaffold: 017109176 Start: 43674 End: 46465 |
| Description | Glycosyl hydrolase superfamily protein |
| View CDS | |
| External Links |
|---|
| NCBI Taxonomy |
| CAZyDB |
| Signature Domain Download full data set without filtering | |||
|---|---|---|---|
| Family | Start | End | Evalue |
| GH5 | 39 | 358 | 2.7e-33 |
| GVPYYGNGFNAYWLMYMATDPSEREKVSSAFREATKNGLTIARTWAFADGGYRALQTSPGSYDEKTFQGLDFVIAEAKKNGIKVMLSLVNNYDDFGGKKQ YVEWARSQGQSLSSEDDFFTNSVVKGFYKNHIKTVLTRINTLTGVAYKDEPTIMAWELMNEPRCTSDPSGKTIQAWITEMASYLKSIDGNHLLEVGLEGF YGPSNPKTNPNYYQIGTDFLANNQIPGIDFATVHSYPDQWLNGSSYEDQVSFLNSWVNDHIHDAQNILKKPVLFAEFGRSLKESGYTINQRDRIFTTVYS GIYSSARGGGPAVGGLFWQL | |||
| Full Sequence |
|---|
| Protein Sequence Length: 430 Download |
| MKHLGVIFFG ALLFIFLNHG VIAEDGFVKT RGVQLVLNGV PYYGNGFNAY WLMYMATDPS 60 EREKVSSAFR EATKNGLTIA RTWAFADGGY RALQTSPGSY DEKTFQGLDF VIAEAKKNGI 120 KVMLSLVNNY DDFGGKKQYV EWARSQGQSL SSEDDFFTNS VVKGFYKNHI KTVLTRINTL 180 TGVAYKDEPT IMAWELMNEP RCTSDPSGKT IQAWITEMAS YLKSIDGNHL LEVGLEGFYG 240 PSNPKTNPNY YQIGTDFLAN NQIPGIDFAT VHSYPDQWLN GSSYEDQVSF LNSWVNDHIH 300 DAQNILKKPV LFAEFGRSLK ESGYTINQRD RIFTTVYSGI YSSARGGGPA VGGLFWQLLA 360 QGMDSFQDGY GVVLSESSST VGLIAEESQK LIKIRKMYAR LRNIEKWKRA REIRRDQFWS 420 GNKVSGTGN* 480 |
| Functional Domains Download unfiltered results here | ||||||||
|---|---|---|---|---|---|---|---|---|
| Cdd ID | Domain | E-Value | Start | End | Length | Domain Description | ||
| cd11315 | AmyAc_bac1_AmyA | 0.002 | 84 | 159 | 101 | + Alpha amylase catalytic domain found in bacterial Alpha-amylases (also called 1,4-alpha-D-glucan-4-glucanohydrolase). AmyA (EC 3.2.1.1) catalyzes the hydrolysis of alpha-(1,4) glycosidic linkages of glycogen, starch, related polysaccharides, and some oligosaccharides. This group includes Firmicutes, Proteobacteria, Actinobacteria, and Cyanobacteria. The Alpha-amylase family comprises the largest family of glycoside hydrolases (GH), with the majority of enzymes acting on starch, glycogen, and related oligo- and polysaccharides. These proteins catalyze the transformation of alpha-1,4 and alpha-1,6 glucosidic linkages with retention of the anomeric center. The protein is described as having 3 domains: A, B, C. A is a (beta/alpha) 8-barrel; B is a loop between the beta 3 strand and alpha 3 helix of A; C is the C-terminal extension characterized by a Greek key. The majority of the enzymes have an active site cleft found between domains A and B where a triad of catalytic residues (Asp, Glu and Asp) performs catalysis. Other members of this family have lost the catalytic activity as in the case of the human 4F2hc, or only have 2 residues that serve as the catalytic nucleophile and the acid/base, such as Thermus A4 beta-galactosidase with 2 Glu residues (GH42) and human alpha-galactosidase with 2 Asp residues (GH31). The family members are quite extensive and include: alpha amylase, maltosyltransferase, cyclodextrin glycotransferase, maltogenic amylase, neopullulanase, isoamylase, 1,4-alpha-D-glucan maltotetrahydrolase, 4-alpha-glucotransferase, oligo-1,6-glucosidase, amylosucrase, sucrose phosphorylase, and amylomaltase. | ||
| pfam00150 | Cellulase | 2.0e-5 | 67 | 316 | 264 | + Cellulase (glycosyl hydrolase family 5). | ||
| COG3934 | COG3934 | 3.0e-28 | 31 | 407 | 384 | + Endo-beta-mannanase [Carbohydrate transport and metabolism] | ||
| Gene Ontology | |
|---|---|
| GO Term | Description |
| GO:0004553 | hydrolase activity, hydrolyzing O-glycosyl compounds |
| GO:0005975 | carbohydrate metabolic process |
| Annotations - NR Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| GenBank | ABV32547.1 | 0 | 1 | 429 | 1 | 431 | endo-1,4-beta-mannosidase protein 1 [Prunus persica] |
| GenBank | ACN78663.1 | 0 | 2 | 429 | 1 | 428 | endo-beta-mannanase [Malus x domestica] |
| GenBank | ACN78664.1 | 0 | 1 | 429 | 1 | 429 | endo-beta-mannanase [Malus x domestica] |
| RefSeq | XP_002281804.1 | 0 | 21 | 429 | 23 | 433 | PREDICTED: hypothetical protein [Vitis vinifera] |
| RefSeq | XP_002510418.1 | 0 | 1 | 429 | 1 | 432 | hydrolase, hydrolyzing O-glycosyl compounds, putative [Ricinus communis] |
| Annotations - PDB Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| PDB | 1rh9_A | 0 | 23 | 394 | 2 | 372 | A Chain A, Family Gh5 Endo-Beta-Mannanase From Lycopersicon Esculentum (Tomato) |
| PDB | 3pzq_A | 0 | 36 | 370 | 21 | 356 | A Chain A, Family Gh5 Endo-Beta-Mannanase From Lycopersicon Esculentum (Tomato) |
| PDB | 3pzo_A | 0 | 36 | 370 | 21 | 356 | A Chain A, Family Gh5 Endo-Beta-Mannanase From Lycopersicon Esculentum (Tomato) |
| PDB | 3pzn_A | 0 | 36 | 370 | 21 | 356 | A Chain A, Family Gh5 Endo-Beta-Mannanase From Lycopersicon Esculentum (Tomato) |
| PDB | 3pzm_A | 0 | 36 | 370 | 21 | 356 | A Chain A, Family Gh5 Endo-Beta-Mannanase From Lycopersicon Esculentum (Tomato) |
