| Basic Information | |
|---|---|
| Species | Medicago truncatula |
| Cazyme ID | Medtr1g015910.1 |
| Family | GH32 |
| Protein Properties | Length: 581 Molecular Weight: 65741 Isoelectric Point: 5.2685 |
| Chromosome | Chromosome/Scaffold: 1 Start: 4518932 End: 4522894 |
| Description | beta-fructofuranosidase 5 |
| View CDS | |
| External Links |
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| NCBI Taxonomy |
| Plaza |
| CAZyDB |
| Signature Domain Download full data set without filtering | |||
|---|---|---|---|
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| Family | Start | End | Evalue |
| GH32 | 50 | 368 | 0 |
| HFQPPQNWMNDPNAPMYYKGVYHLFYQHNPLAATFGDIIVWAHSVSYDLINWIHLNIALEPSGPYDINSCWSGSATILPGEKPAILYTGIDHYKNEVQNL AIPKNLTDAFLREWEKHPQNPVMTPPTGVEEDNFRDPSTAWHGKDGKWRVIVGAQNGDEGKTILYQSEDFVNWTVNPNPFFATDNTGVCECPDFFPVYIN STNGVDTSVDNSSVRHVLKISYLRKQHDYYFLGKYVSDKENFVPDVEFTGTSKDLRFDYGKFYASKSFFDYAKNRRILWGWVNESDSTQDDIEKGWAGLQ TIPRKVWLDKSGKRLMQWP | |||
| Full Sequence |
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| Protein Sequence Length: 581 Download |
| MKNFAVQLLL LLISFLLNSR NGIEASTCSN SIDPIKYRIP KEQPYRTSYH FQPPQNWMND 60 PNAPMYYKGV YHLFYQHNPL AATFGDIIVW AHSVSYDLIN WIHLNIALEP SGPYDINSCW 120 SGSATILPGE KPAILYTGID HYKNEVQNLA IPKNLTDAFL REWEKHPQNP VMTPPTGVEE 180 DNFRDPSTAW HGKDGKWRVI VGAQNGDEGK TILYQSEDFV NWTVNPNPFF ATDNTGVCEC 240 PDFFPVYINS TNGVDTSVDN SSVRHVLKIS YLRKQHDYYF LGKYVSDKEN FVPDVEFTGT 300 SKDLRFDYGK FYASKSFFDY AKNRRILWGW VNESDSTQDD IEKGWAGLQT IPRKVWLDKS 360 GKRLMQWPIE EIENLRHKQI SITGEKLEGG STLEISGINV SQVDVEVLFE LPELESGDWL 420 EDPSEVDPQL LCSKQHASRS GKIGPFGLLA LASKDLTEET AVSFQIFRAS NRYLCLMCSD 480 QSRSSLRNDL DKTTYGTIFD IDPNLKTISL RTLIDKSIIE SFGDGGKAVI TSRVYPLLAI 540 EKDAHLYVFN NGSQSVVISK LNAWSMKQAE IGHEGNINCA * |
| Functional Domains Download unfiltered results here | ||||||||
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| Cdd ID | Domain | E-Value | Start | End | Length | Domain Description | ||
| TIGR01322 | scrB_fam | 9.0e-58 | 44 | 536 | 510 | + sucrose-6-phosphate hydrolase. [Energy metabolism, Biosynthesis and degradation of polysaccharides]. | ||
| COG1621 | SacC | 5.0e-79 | 35 | 536 | 516 | + Beta-fructosidases (levanase/invertase) [Carbohydrate transport and metabolism] | ||
| cd08996 | GH32_B_Fructosidase | 3.0e-96 | 56 | 371 | 328 | + Glycosyl hydrolase family 32, beta-fructosidases. Glycosyl hydrolase family GH32 cleaves sucrose into fructose and glucose via beta-fructofuranosidase activity, producing invert sugar that is a mixture of dextrorotatory D-glucose and levorotatory D-fructose, thus named invertase (EC 3.2.1.26). This family also contains other fructofuranosidases such as inulinase (EC 3.2.1.7), exo-inulinase (EC 3.2.1.80), levanase (EC 3.2.1.65), and transfructosidases such sucrose:sucrose 1-fructosyltransferase (EC 2.4.1.99), fructan:fructan 1-fructosyltransferase (EC 2.4.1.100), sucrose:fructan 6-fructosyltransferase (EC 2.4.1.10), fructan:fructan 6G-fructosyltransferase (EC 2.4.1.243) and levan fructosyltransferases (EC 2.4.1.-). These retaining enzymes (i.e. they retain the configuration at anomeric carbon atom of the substrate) catalyze hydrolysis in two steps involving a covalent glycosyl enzyme intermediate: an aspartate located close to the N-terminus acts as the catalytic nucleophile and a glutamate acts as the general acid/base; a conserved aspartate residue in the Arg-Asp-Pro (RDP) motif stabilizes the transition state. These enzymes are predicted to display a 5-fold beta-propeller fold as found for GH43 and CH68. The breakdown of sucrose is widely used as a carbon or energy source by bacteria, fungi, and plants. Invertase is used commercially in the confectionery industry, since fructose has a sweeter taste than sucrose and a lower tendency to crystallize. A common structural feature of all these enzymes is a 5-bladed beta-propeller domain, similar to GH43, that contains the catalytic acid and catalytic base. A long V-shaped groove, partially enclosed at one end, forms a single extended substrate-binding surface across the face of the propeller. | ||
| pfam00251 | Glyco_hydro_32N | 1.0e-142 | 50 | 368 | 327 | + Glycosyl hydrolases family 32 N-terminal domain. This domain corresponds to the N-terminal domain of glycosyl hydrolase family 32 which forms a five bladed beta propeller structure. | ||
| smart00640 | Glyco_32 | 4.0e-167 | 50 | 526 | 487 | + Glycosyl hydrolases family 32. | ||
| Annotations - NR Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| EMBL | CAC37923.1 | 0 | 42 | 580 | 42 | 580 | fructan 1-exohydrolase IIb [Cichorium intybus] |
| EMBL | CAD49079.1 | 0 | 35 | 571 | 35 | 568 | fructan 1-exohydrolase [Campanula rapunculoides] |
| RefSeq | XP_002278918.1 | 0 | 4 | 571 | 7 | 559 | PREDICTED: hypothetical protein isoform 3 [Vitis vinifera] |
| RefSeq | XP_002309496.1 | 0 | 42 | 571 | 34 | 559 | predicted protein [Populus trichocarpa] |
| RefSeq | XP_002309497.1 | 0 | 22 | 577 | 24 | 572 | predicted protein [Populus trichocarpa] |
| Annotations - PDB Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| PDB | 2aey_A | 0 | 42 | 580 | 4 | 542 | A Chain A, The Crystal Structure Of A Hyperthermoactive Exopolygalacturonase From Thermotoga Maritima |
| PDB | 2ade_A | 0 | 42 | 580 | 4 | 542 | A Chain A, The Crystal Structure Of A Hyperthermoactive Exopolygalacturonase From Thermotoga Maritima |
| PDB | 2add_A | 0 | 42 | 580 | 4 | 542 | A Chain A, The Crystal Structure Of A Hyperthermoactive Exopolygalacturonase From Thermotoga Maritima |
| PDB | 1st8_A | 0 | 42 | 580 | 4 | 542 | A Chain A, Crystal Structure Of Fructan 1-Exohydrolase Iia From Cichorium Intybus |
| PDB | 2aez_A | 0 | 42 | 580 | 4 | 542 | A Chain A, Crystal Structure Of Fructan 1-Exohydrolase Iia (E201q) From Cichorium Intybus In Complex With 1-Kestose |
