Basic Information | |
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Species | Medicago truncatula |
Cazyme ID | Medtr1g015940.1 |
Family | GH32 |
Protein Properties | Length: 610 Molecular Weight: 68852.9 Isoelectric Point: 5.9257 |
Chromosome | Chromosome/Scaffold: 1 Start: 4530088 End: 4535823 |
Description | Glycosyl hydrolases family 32 protein |
View CDS |
External Links |
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NCBI Taxonomy |
Plaza |
CAZyDB |
Signature Domain Download full data set without filtering | |||
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Family | Start | End | Evalue |
GH32 | 129 | 401 | 0 |
SFTYNPAAATFGHEKMVWGHSISNDLINWTHLNDAIVPTIPGDINSCWSGSATILPGEKPAMLYTGIDQNRHQVQNLAMPKNLSDPYLREWEKHPQNPLM TPPSGVEVGEFRDPSTAWQGKDGKWRVIIGAQNGDEGKIILYKSEDFVKWIVDPIPFFATDDTGVCECPDFFTVYINSTNGVDTTMENSSVRHEKFIPDV NYTGTCKDLRFDYGLFYASKSFFDYAKNRRILWGWVEENDSEQDEIDKGWAGLQTIPRKFWLDESGERLMQWP |
Full Sequence |
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Protein Sequence Length: 610 Download |
MHLGVICVEY ESHEECKEQS LGDSGSIFKY VLIHVNPHSL SFLAVTGPMY YKGVYHLFYQ 60 YNVSLNSLHA TSHLSSMQTP RVQLVTCISC ILQHVNACNH PLCHHALHEK CNFSFMHHGH 120 VSCYKSAVSF TYNPAAATFG HEKMVWGHSI SNDLINWTHL NDAIVPTIPG DINSCWSGSA 180 TILPGEKPAM LYTGIDQNRH QVQNLAMPKN LSDPYLREWE KHPQNPLMTP PSGVEVGEFR 240 DPSTAWQGKD GKWRVIIGAQ NGDEGKIILY KSEDFVKWIV DPIPFFATDD TGVCECPDFF 300 TVYINSTNGV DTTMENSSVR HEKFIPDVNY TGTCKDLRFD YGLFYASKSF FDYAKNRRIL 360 WGWVEENDSE QDEIDKGWAG LQTIPRKFWL DESGERLMQW PIEELEKLGG NQINITGETL 420 QSGSTLEVKG ITASQADVEV LFGLPDLQSA ELLEPSETNP QELCKEQYAS RKCMIGPFGL 480 QALASKDQTE KTTISFRIYR VADHYKCLMI SDQTRSSLRQ DLKKLIYATI FDIDPNLKTI 540 SLRSLIDRSI IESFGDGGKA CITNRVYPLL AIEKYAHLYV FNNGSQSVAI SQLNAWSMKQ 600 AEFRVENSI* |
Functional Domains Download unfiltered results here | ||||||||
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Cdd ID | Domain | E-Value | Start | End | Length | Domain Description | ||
smart00640 | Glyco_32 | 2.0e-5 | 47 | 62 | 16 | + Glycosyl hydrolases family 32. | ||
pfam00251 | Glyco_hydro_32N | 8.0e-6 | 47 | 62 | 16 | + Glycosyl hydrolases family 32 N-terminal domain. This domain corresponds to the N-terminal domain of glycosyl hydrolase family 32 which forms a five bladed beta propeller structure. | ||
cd08996 | GH32_B_Fructosidase | 3.0e-70 | 132 | 404 | 293 | + Glycosyl hydrolase family 32, beta-fructosidases. Glycosyl hydrolase family GH32 cleaves sucrose into fructose and glucose via beta-fructofuranosidase activity, producing invert sugar that is a mixture of dextrorotatory D-glucose and levorotatory D-fructose, thus named invertase (EC 3.2.1.26). This family also contains other fructofuranosidases such as inulinase (EC 3.2.1.7), exo-inulinase (EC 3.2.1.80), levanase (EC 3.2.1.65), and transfructosidases such sucrose:sucrose 1-fructosyltransferase (EC 2.4.1.99), fructan:fructan 1-fructosyltransferase (EC 2.4.1.100), sucrose:fructan 6-fructosyltransferase (EC 2.4.1.10), fructan:fructan 6G-fructosyltransferase (EC 2.4.1.243) and levan fructosyltransferases (EC 2.4.1.-). These retaining enzymes (i.e. they retain the configuration at anomeric carbon atom of the substrate) catalyze hydrolysis in two steps involving a covalent glycosyl enzyme intermediate: an aspartate located close to the N-terminus acts as the catalytic nucleophile and a glutamate acts as the general acid/base; a conserved aspartate residue in the Arg-Asp-Pro (RDP) motif stabilizes the transition state. These enzymes are predicted to display a 5-fold beta-propeller fold as found for GH43 and CH68. The breakdown of sucrose is widely used as a carbon or energy source by bacteria, fungi, and plants. Invertase is used commercially in the confectionery industry, since fructose has a sweeter taste than sucrose and a lower tendency to crystallize. A common structural feature of all these enzymes is a 5-bladed beta-propeller domain, similar to GH43, that contains the catalytic acid and catalytic base. A long V-shaped groove, partially enclosed at one end, forms a single extended substrate-binding surface across the face of the propeller. | ||
pfam00251 | Glyco_hydro_32N | 1.0e-95 | 132 | 401 | 285 | + Glycosyl hydrolases family 32 N-terminal domain. This domain corresponds to the N-terminal domain of glycosyl hydrolase family 32 which forms a five bladed beta propeller structure. | ||
smart00640 | Glyco_32 | 1.0e-110 | 132 | 558 | 449 | + Glycosyl hydrolases family 32. |
Annotations - NR Download unfiltered results here | |||||||
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Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
PDB | 1ST8 | 0 | 130 | 609 | 37 | 539 | A Chain A, Crystal Structure Of Fructan 1-Exohydrolase Iia From Cichorium Intybus |
EMBL | CAD49079.1 | 0 | 130 | 609 | 75 | 574 | fructan 1-exohydrolase [Campanula rapunculoides] |
RefSeq | XP_002309497.1 | 0 | 130 | 602 | 73 | 565 | predicted protein [Populus trichocarpa] |
Annotations - PDB Download unfiltered results here | |||||||
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Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
PDB | 2aey_A | 0 | 130 | 609 | 37 | 539 | B Chain B, Structural Basis Of Gibberellin(Ga3)-Induced Della Recognition By The Gibberellin Receptor |
PDB | 2ade_A | 0 | 130 | 609 | 37 | 539 | B Chain B, Structural Basis Of Gibberellin(Ga3)-Induced Della Recognition By The Gibberellin Receptor |
PDB | 2add_A | 0 | 130 | 609 | 37 | 539 | B Chain B, Structural Basis Of Gibberellin(Ga3)-Induced Della Recognition By The Gibberellin Receptor |
EST Download unfiltered results here | ||||
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Hit | Length | Start | End | EValue |
BG645655 | 192 | 130 | 321 | 0 |
FE693031 | 279 | 322 | 600 | 0 |
CT842376 | 500 | 130 | 600 | 0 |
DY273985 | 317 | 130 | 421 | 0 |
BG645655 | 17 | 46 | 62 | 0.6 |
Sequence Alignments (This image is cropped. Click for full image.) |
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