Basic Information | |
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Species | Medicago truncatula |
Cazyme ID | Medtr1g015970.1 |
Family | GH32 |
Protein Properties | Length: 552 Molecular Weight: 62960.8 Isoelectric Point: 5.0404 |
Chromosome | Chromosome/Scaffold: 1 Start: 4538820 End: 4542040 |
Description | Glycosyl hydrolases family 32 protein |
View CDS |
External Links |
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NCBI Taxonomy |
Plaza |
CAZyDB |
Signature Domain Download full data set without filtering | |||
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Family | Start | End | Evalue |
GH32 | 24 | 343 | 0 |
HFQPLKNWMNDPNGPMYYKGVYHLFYQYNPDAATFGHEKMVWGHSISNDLINWTHLNDAIVPTIPGDINSCWSGSATILPGEKPAMLYTGIDQNRHQVQN LAMPKNLSDPYLREWEKHPQNPLMTPPSGVEVGEFRDPSTAWQGKDGKWRVIIGAQNGDEGKIILYKSEDFVKWIVDPIPFFATDDTGVCECPDFFPVYI NNTNGVDTTMENSSVRHVLKISYLRRRHDYYFIGKYVSDKEEFVPDVKYTGTWKELRFDYGKVYASKSFFDHAKNRRILWAWAEESDTSEDDIQKGWAGL QTIPRKFWLDKNGKQLMQWP |
Full Sequence |
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Protein Sequence Length: 552 Download |
MENIREDASP NNINSEQPYR TWYHFQPLKN WMNDPNGPMY YKGVYHLFYQ YNPDAATFGH 60 EKMVWGHSIS NDLINWTHLN DAIVPTIPGD INSCWSGSAT ILPGEKPAML YTGIDQNRHQ 120 VQNLAMPKNL SDPYLREWEK HPQNPLMTPP SGVEVGEFRD PSTAWQGKDG KWRVIIGAQN 180 GDEGKIILYK SEDFVKWIVD PIPFFATDDT GVCECPDFFP VYINNTNGVD TTMENSSVRH 240 VLKISYLRRR HDYYFIGKYV SDKEEFVPDV KYTGTWKELR FDYGKVYASK SFFDHAKNRR 300 ILWAWAEESD TSEDDIQKGW AGLQTIPRKF WLDKNGKQLM QWPIEELEKL RGKQINITGE 360 TLQSGSTLEV KGITASQADV EVLFELPNLQ SAELLEPSEV DPQELCKEQY ASRNGMIGPF 420 GLQALASKDQ TERTTISFRI YRAGDGFKCL MISDQTRSSL REDVEKTSYA TIFDIDPNLK 480 TISLRSLIDR SIIESFGDGG RACITSRAYP LFATDKDAHL FVFNDGSQSV VISQLNAWSM 540 KQAQFGTESI I* |
Functional Domains Download unfiltered results here | ||||||||
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Cdd ID | Domain | E-Value | Start | End | Length | Domain Description | ||
TIGR01322 | scrB_fam | 2.0e-51 | 18 | 395 | 401 | + sucrose-6-phosphate hydrolase. [Energy metabolism, Biosynthesis and degradation of polysaccharides]. | ||
COG1621 | SacC | 1.0e-68 | 16 | 385 | 384 | + Beta-fructosidases (levanase/invertase) [Carbohydrate transport and metabolism] | ||
cd08996 | GH32_B_Fructosidase | 6.0e-94 | 30 | 346 | 329 | + Glycosyl hydrolase family 32, beta-fructosidases. Glycosyl hydrolase family GH32 cleaves sucrose into fructose and glucose via beta-fructofuranosidase activity, producing invert sugar that is a mixture of dextrorotatory D-glucose and levorotatory D-fructose, thus named invertase (EC 3.2.1.26). This family also contains other fructofuranosidases such as inulinase (EC 3.2.1.7), exo-inulinase (EC 3.2.1.80), levanase (EC 3.2.1.65), and transfructosidases such sucrose:sucrose 1-fructosyltransferase (EC 2.4.1.99), fructan:fructan 1-fructosyltransferase (EC 2.4.1.100), sucrose:fructan 6-fructosyltransferase (EC 2.4.1.10), fructan:fructan 6G-fructosyltransferase (EC 2.4.1.243) and levan fructosyltransferases (EC 2.4.1.-). These retaining enzymes (i.e. they retain the configuration at anomeric carbon atom of the substrate) catalyze hydrolysis in two steps involving a covalent glycosyl enzyme intermediate: an aspartate located close to the N-terminus acts as the catalytic nucleophile and a glutamate acts as the general acid/base; a conserved aspartate residue in the Arg-Asp-Pro (RDP) motif stabilizes the transition state. These enzymes are predicted to display a 5-fold beta-propeller fold as found for GH43 and CH68. The breakdown of sucrose is widely used as a carbon or energy source by bacteria, fungi, and plants. Invertase is used commercially in the confectionery industry, since fructose has a sweeter taste than sucrose and a lower tendency to crystallize. A common structural feature of all these enzymes is a 5-bladed beta-propeller domain, similar to GH43, that contains the catalytic acid and catalytic base. A long V-shaped groove, partially enclosed at one end, forms a single extended substrate-binding surface across the face of the propeller. | ||
pfam00251 | Glyco_hydro_32N | 2.0e-138 | 24 | 343 | 328 | + Glycosyl hydrolases family 32 N-terminal domain. This domain corresponds to the N-terminal domain of glycosyl hydrolase family 32 which forms a five bladed beta propeller structure. | ||
smart00640 | Glyco_32 | 3.0e-153 | 24 | 500 | 487 | + Glycosyl hydrolases family 32. |
Annotations - NR Download unfiltered results here | |||||||
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Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
PDB | 1ST8 | 0 | 16 | 545 | 4 | 533 | A Chain A, Crystal Structure Of Fructan 1-Exohydrolase Iia From Cichorium Intybus |
PDB | 2AEZ | 0 | 16 | 545 | 4 | 533 | A Chain A, Crystal Structure Of Fructan 1-Exohydrolase Iia (E201q) From Cichorium Intybus In Complex With 1-Kestose |
EMBL | CAC37922.1 | 0 | 1 | 545 | 18 | 571 | fructan 1-exohydrolase IIa [Cichorium intybus] |
RefSeq | XP_002309496.1 | 0 | 8 | 545 | 26 | 559 | predicted protein [Populus trichocarpa] |
RefSeq | XP_002309497.1 | 0 | 13 | 544 | 40 | 565 | predicted protein [Populus trichocarpa] |
Annotations - PDB Download unfiltered results here | |||||||
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Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
PDB | 2aey_A | 0 | 16 | 545 | 4 | 533 | A Chain A, Crystal Structure At 1.5a Resolution Of The Barley Alpha- Amylase Isozyme 1 |
PDB | 2ade_A | 0 | 16 | 545 | 4 | 533 | A Chain A, Crystal Structure At 1.5a Resolution Of The Barley Alpha- Amylase Isozyme 1 |
PDB | 2add_A | 0 | 16 | 545 | 4 | 533 | A Chain A, Crystal Structure At 1.5a Resolution Of The Barley Alpha- Amylase Isozyme 1 |
PDB | 1st8_A | 0 | 16 | 545 | 4 | 533 | A Chain A, Crystal Structure Of Fructan 1-Exohydrolase Iia From Cichorium Intybus |
PDB | 2aez_A | 0 | 16 | 545 | 4 | 533 | A Chain A, Crystal Structure Of Fructan 1-Exohydrolase Iia (E201q) From Cichorium Intybus In Complex With 1-Kestose |
EST Download unfiltered results here | ||||
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Hit | Length | Start | End | EValue |
BG645655 | 262 | 2 | 263 | 0 |
CT842376 | 532 | 20 | 542 | 0 |
FE693031 | 283 | 260 | 542 | 0 |
CA921732 | 202 | 348 | 549 | 0 |
DY273985 | 327 | 39 | 363 | 0 |
Sequence Alignments (This image is cropped. Click for full image.) |
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