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Basic Information | |
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Species | Medicago truncatula |
Cazyme ID | Medtr1g015980.1 |
Family | GH32 |
Protein Properties | Length: 560 Molecular Weight: 63982.9 Isoelectric Point: 4.581 |
Chromosome | Chromosome/Scaffold: 1 Start: 4543878 End: 4546678 |
Description | Glycosyl hydrolases family 32 protein |
View CDS |
External Links |
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NCBI Taxonomy |
Plaza |
CAZyDB |
Signature Domain Download full data set without filtering | |||
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Family | Start | End | Evalue |
GH32 | 24 | 347 | 0 |
HFQPKKNWMNDPNGPMYYKGFYHFFYQHNPDGASFGVNKMVWGHSISKDLINWTHLNHAIEPTCAGETSCFSGSATIVPGEQPVIYMLYTGLINEKTHQV QYLAMPKDLSDPKLIEWIKHPQNPLMAAPNGVEVGEFRDPSTAWQGKDGKWRVLIGARNGEQGKAILYRSEDFVNWIVDPNPFYATDGTGVCECPDFFPV YINSTNGVDTSVENSSVRHVFKISYLLRCHDYYFIGKYVSDSDQEKFIPDEKFTGTWKELIFDYGNFYASKSFFDYAKNRRILWAWVLESDTKEDGIERG WAGLQTIPRKFWLDESGKRLLQWP |
Full Sequence |
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Protein Sequence Length: 560 Download |
MENIVEDAPP NNINLEQPYR TWYHFQPKKN WMNDPNGPMY YKGFYHFFYQ HNPDGASFGV 60 NKMVWGHSIS KDLINWTHLN HAIEPTCAGE TSCFSGSATI VPGEQPVIYM LYTGLINEKT 120 HQVQYLAMPK DLSDPKLIEW IKHPQNPLMA APNGVEVGEF RDPSTAWQGK DGKWRVLIGA 180 RNGEQGKAIL YRSEDFVNWI VDPNPFYATD GTGVCECPDF FPVYINSTNG VDTSVENSSV 240 RHVFKISYLL RCHDYYFIGK YVSDSDQEKF IPDEKFTGTW KELIFDYGNF YASKSFFDYA 300 KNRRILWAWV LESDTKEDGI ERGWAGLQTI PRKFWLDESG KRLLQWPIEE LEQLRYNQIN 360 ITRETLLSGS TLEVIGITAS QADVEVLFEL PDLESAEVLE PSEVDPQELC KEQYASIKGM 420 IGPFGLQALA SEDQTERTTI SFRIYRVSDE YKCLMISDQT RSSSSLREGL EKPIYATIFD 480 IDPNVKTISL RSLIDRSIIE SFGDGGKVVI TSRVYPLLAI EKDAHLFVFN DGSQSVVISE 540 LNAWSMNQAE FENEQTYYG* 600 |
Functional Domains Download unfiltered results here | ||||||||
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Cdd ID | Domain | E-Value | Start | End | Length | Domain Description | ||
TIGR01322 | scrB_fam | 3.0e-49 | 15 | 516 | 519 | + sucrose-6-phosphate hydrolase. [Energy metabolism, Biosynthesis and degradation of polysaccharides]. | ||
COG1621 | SacC | 7.0e-64 | 16 | 389 | 390 | + Beta-fructosidases (levanase/invertase) [Carbohydrate transport and metabolism] | ||
cd08996 | GH32_B_Fructosidase | 2.0e-84 | 30 | 350 | 333 | + Glycosyl hydrolase family 32, beta-fructosidases. Glycosyl hydrolase family GH32 cleaves sucrose into fructose and glucose via beta-fructofuranosidase activity, producing invert sugar that is a mixture of dextrorotatory D-glucose and levorotatory D-fructose, thus named invertase (EC 3.2.1.26). This family also contains other fructofuranosidases such as inulinase (EC 3.2.1.7), exo-inulinase (EC 3.2.1.80), levanase (EC 3.2.1.65), and transfructosidases such sucrose:sucrose 1-fructosyltransferase (EC 2.4.1.99), fructan:fructan 1-fructosyltransferase (EC 2.4.1.100), sucrose:fructan 6-fructosyltransferase (EC 2.4.1.10), fructan:fructan 6G-fructosyltransferase (EC 2.4.1.243) and levan fructosyltransferases (EC 2.4.1.-). These retaining enzymes (i.e. they retain the configuration at anomeric carbon atom of the substrate) catalyze hydrolysis in two steps involving a covalent glycosyl enzyme intermediate: an aspartate located close to the N-terminus acts as the catalytic nucleophile and a glutamate acts as the general acid/base; a conserved aspartate residue in the Arg-Asp-Pro (RDP) motif stabilizes the transition state. These enzymes are predicted to display a 5-fold beta-propeller fold as found for GH43 and CH68. The breakdown of sucrose is widely used as a carbon or energy source by bacteria, fungi, and plants. Invertase is used commercially in the confectionery industry, since fructose has a sweeter taste than sucrose and a lower tendency to crystallize. A common structural feature of all these enzymes is a 5-bladed beta-propeller domain, similar to GH43, that contains the catalytic acid and catalytic base. A long V-shaped groove, partially enclosed at one end, forms a single extended substrate-binding surface across the face of the propeller. | ||
pfam00251 | Glyco_hydro_32N | 1.0e-123 | 24 | 347 | 333 | + Glycosyl hydrolases family 32 N-terminal domain. This domain corresponds to the N-terminal domain of glycosyl hydrolase family 32 which forms a five bladed beta propeller structure. | ||
smart00640 | Glyco_32 | 8.0e-138 | 24 | 506 | 490 | + Glycosyl hydrolases family 32. |
Annotations - NR Download unfiltered results here | |||||||
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Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
PDB | 1ST8 | 0 | 15 | 556 | 3 | 538 | A Chain A, Crystal Structure Of Fructan 1-Exohydrolase Iia From Cichorium Intybus |
PDB | 2AEZ | 0 | 15 | 556 | 3 | 538 | A Chain A, Crystal Structure Of Fructan 1-Exohydrolase Iia (E201q) From Cichorium Intybus In Complex With 1-Kestose |
EMBL | CAC37922.1 | 0 | 12 | 556 | 38 | 576 | fructan 1-exohydrolase IIa [Cichorium intybus] |
EMBL | CAD49079.1 | 0 | 16 | 556 | 42 | 573 | fructan 1-exohydrolase [Campanula rapunculoides] |
RefSeq | XP_002309497.1 | 0 | 3 | 557 | 28 | 572 | predicted protein [Populus trichocarpa] |
Annotations - PDB Download unfiltered results here | |||||||
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Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
PDB | 2aey_A | 0 | 15 | 556 | 3 | 538 | A Chain A, Hevamine Mutant D125aY183F IN COMPLEX WITH TETRA-Nag |
PDB | 2ade_A | 0 | 15 | 556 | 3 | 538 | A Chain A, Hevamine Mutant D125aY183F IN COMPLEX WITH TETRA-Nag |
PDB | 2add_A | 0 | 15 | 556 | 3 | 538 | A Chain A, Hevamine Mutant D125aY183F IN COMPLEX WITH TETRA-Nag |
PDB | 1st8_A | 0 | 15 | 556 | 3 | 538 | A Chain A, Crystal Structure Of Fructan 1-Exohydrolase Iia From Cichorium Intybus |
PDB | 2aez_A | 0 | 15 | 556 | 3 | 538 | A Chain A, Crystal Structure Of Fructan 1-Exohydrolase Iia (E201q) From Cichorium Intybus In Complex With 1-Kestose |
EST Download unfiltered results here | ||||
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Hit | Length | Start | End | EValue |
BG645338 | 212 | 1 | 212 | 0 |
AW736408 | 207 | 1 | 207 | 0 |
BG645655 | 256 | 10 | 264 | 0 |
AW773822 | 197 | 1 | 197 | 0 |
AW736101 | 197 | 12 | 208 | 0 |
Sequence Alignments (This image is cropped. Click for full image.) |
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