| Basic Information | |
|---|---|
| Species | Medicago truncatula |
| Cazyme ID | Medtr2g006960.1 |
| Family | GT24 |
| Protein Properties | Length: 1651 Molecular Weight: 187295 Isoelectric Point: 5.7999 |
| Chromosome | Chromosome/Scaffold: 2 Start: 860460 End: 877417 |
| Description | UDP-glucose:glycoprotein glucosyltransferases;transferases, transferring hexosyl groups;transferases, transferring glycosyl groups |
| View CDS | |
| External Links |
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| NCBI Taxonomy |
| Plaza |
| CAZyDB |
| Signature Domain Download full data set without filtering | |||
|---|---|---|---|
| Family | Start | End | Evalue |
| GT24 | 1346 | 1593 | 0 |
| INIFSIASGHLYERFLKIMILSVLKNTHRPVKFWFIKNYLSPPFKDLIPHMSQEYGFEYELITYKWPTWLHKQKEKQRIIWAYKILFLDVIFPLSLEKVI FVDADQIVRTDMGELYDMDLKGRPLAYTPFCDNNREMDGYRFWRQGFWKDHLRGRPYHISALYVVDLKKFRETAAGDNLRVFYETLSKDPNSLANLDQDL PNYAQHTVPIFSLPQEWLWCESWCGNATKSKAKTIDLCNNPMTKEPKL | |||
| Full Sequence |
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| Protein Sequence Length: 1651 Download |
| MRFRSIHFSL LFISITSFSL LLLLLLVTAD TRSPKNVQTA LRAKWSGTPL LLEASELLSK 60 QQQHYFWNFI DIWINANDDA NPDANANAKY CVKKILEHGR SLLTEPLASI FEFSLILRSA 120 SPTLVLYRQL ARDSLSSFPL FHNDNEIAEI KKNETQLDPL RVGVSVESPG GKCCWVDTGE 180 HLFFDVDELR SWLQNNHDHQ KVGNSFQSPP VFEFDHIHFD SATGSPVAIL YGALGTNCFK 240 EFHVALLEAA KQRKVKYVLR PVLPAGCDAQ IGPCGSVGVS ESVNLGGYGV ELALKNMEYK 300 AMDDSAVKKG VTLEDPRIED LSQEVRGFIF SKILDRKPEL ASEIMAFRDY LLSSTVSDTL 360 DVWELKDLGH QTVQRIVRAS DPLQSMQDIN QNFPSIVSYL SRMKLDDSVR DEITANQRMI 420 PPGKSLMAIN GALVNVEDID LYMLIDLVHQ DLLLADQFSK LKIPPSIVQK LLSTLPPPES 480 DMFRLDFRST HVHYLNNLEE DGKYKWWRSN LNEILMPVFP GQLRQIRKNL FHAVFVLDPA 540 TTYGLESIDM IMSLHENSFP VRFGVVLYSS KYITQLEDHS TKEDGDKFAG DISDMIIRLF 600 SYIKGNYGIE MAFKFLSNVN KLRIESDDNV EDAHLEQHHV ESAFVETVLP KVKSPPQEIL 660 LKLEKEPELK ELSQESSKLV FKLGLSKIQC SLLMNGLVID PNEEALMNAL NDETQRIQEQ 720 VYFGQIKSHT DVLDKFLSEA GIQRYNPRII ADNKPKFISL SMFTFGEASI LKRINYLHSS 780 GTMDDLKPVT HLLAVDITSG SGIKLLRQGL NYLIEGSKDA RVGLLFSGNQ TTNLFSLLFV 840 KVFEITTSSY SHKKNALDFL DQLSSVYLQK YIRTPALEVD GTQAFIDEVC KLAESNGLPS 900 EGYRSSLSEF SADEARRHLS EVEKFLFTAL GSESGVNAVL TNGRVTSPID ESTFLSADLH 960 LLESIELKKR TKHIVEIIEE MTWDDVDPDM LTRFHFIFAL SVLSCGYYLV SSKFISDIVM 1020 SVSSAMSMRE RSSESARFEV LSDEHSAIIL NNENSSIHID AVLDPLSPTS QKLSGILRVL 1080 WKYIQPSMRI VLNPLSSLAD LPLKNYYRYV VPSMDDFSNI DSSINGPKAF FANMPLSKTL 1140 TMNLDVPEPW LVEPILTVHD LDNILLENLG DTRTLQAVFE LEALVLTGHC SEKDHDPPRG 1200 LQLILGTKTS PHLVDTLVMA NLGYWQMKVA PGVWFLQLAP GRSSELYIFK EDDDGSKNKQ 1260 SSKLITINSL RGKVVHMEVV KRKGKEHEKL LIPDDDDDLQ HKKKGSGWNS NLLKWASGFI 1320 GSNEQSKNAE SNSPENARGG RHGKTINIFS IASGHLYERF LKIMILSVLK NTHRPVKFWF 1380 IKNYLSPPFK DLIPHMSQEY GFEYELITYK WPTWLHKQKE KQRIIWAYKI LFLDVIFPLS 1440 LEKVIFVDAD QIVRTDMGEL YDMDLKGRPL AYTPFCDNNR EMDGYRFWRQ GFWKDHLRGR 1500 PYHISALYVV DLKKFRETAA GDNLRVFYET LSKDPNSLAN LDQDLPNYAQ HTVPIFSLPQ 1560 EWLWCESWCG NATKSKAKTI DLCNNPMTKE PKLQGARRIV AEWPDLDLEA RKFTARILGD 1620 DLEPIQSPDQ SKDSTNEDSL KEDLESKAEL * 1680 |
| Functional Domains Download unfiltered results here | ||||||||
|---|---|---|---|---|---|---|---|---|
| Cdd ID | Domain | E-Value | Start | End | Length | Domain Description | ||
| pfam01501 | Glyco_transf_8 | 3.0e-5 | 1350 | 1545 | 205 | + Glycosyl transferase family 8. This family includes enzymes that transfer sugar residues to donor molecules. Members of this family are involved in lipopolysaccharide biosynthesis and glycogen synthesis. This family includes Lipopolysaccharide galactosyltransferase, lipopolysaccharide glucosyltransferase 1, and glycogenin glucosyltransferase. | ||
| cd04194 | GT8_A4GalT_like | 3.0e-15 | 1346 | 1562 | 227 | + A4GalT_like proteins catalyze the addition of galactose or glucose residues to the lipooligosaccharide (LOS) or lipopolysaccharide (LPS) of the bacterial cell surface. The members of this family of glycosyltransferases catalyze the addition of galactose or glucose residues to the lipooligosaccharide (LOS) or lipopolysaccharide (LPS) of the bacterial cell surface. The enzymes exhibit broad substrate specificities. The known functions found in this family include: Alpha-1,4-galactosyltransferase, LOS-alpha-1,3-D-galactosyltransferase, UDP-glucose:(galactosyl) LPS alpha1,2-glucosyltransferase, UDP-galactose: (glucosyl) LPS alpha1,2-galactosyltransferase, and UDP-glucose:(glucosyl) LPS alpha1,2-glucosyltransferase. Alpha-1,4-galactosyltransferase from N. meningitidis adds an alpha-galactose from UDP-Gal (the donor) to a terminal lactose (the acceptor) of the LOS structure of outer membrane. LOSs are virulence factors that enable the organism to evade the immune system of host cells. In E. coli, the three alpha-1,2-glycosyltransferases, that are involved in the synthesis of the outer core region of the LPS, are all members of this family. The three enzymes share 40 % of sequence identity, but have different sugar donor or acceptor specificities, representing the structural diversity of LPS. | ||
| cd00505 | Glyco_transf_8 | 6.0e-57 | 1346 | 1593 | 257 | + Members of glycosyltransferase family 8 (GT-8) are involved in lipopolysaccharide biosynthesis and glycogen synthesis. Members of this family are involved in lipopolysaccharide biosynthesis and glycogen synthesis. GT-8 comprises enzymes with a number of known activities: lipopolysaccharide galactosyltransferase, lipopolysaccharide glucosyltransferase 1, glycogenin glucosyltransferase, and N-acetylglucosaminyltransferase. GT-8 enzymes contains a conserved DXD motif which is essential in the coordination of a catalytic divalent cation, most commonly Mn2+. | ||
| pfam06427 | UDP-g_GGTase | 1.0e-71 | 1041 | 1221 | 185 | + UDP-glucose:Glycoprotein Glucosyltransferase. The N-terminal region of this group of proteins is required for correct folding of the ER UDP-Glc: glucosyltransferase. | ||
| cd06432 | GT8_HUGT1_C_like | 1.0e-173 | 1346 | 1593 | 248 | + The C-terminal domain of HUGT1-like is highly homologous to the GT 8 family. C-terminal domain of glycoprotein glucosyltransferase (UGT). UGT is a large glycoprotein whose C-terminus contains the catalytic activity. This catalytic C-terminal domain is highly homologous to Glycosyltransferase Family 8 (GT 8) and contains the DXD motif that coordinates donor sugar binding, characteristic for Family 8 glycosyltransferases. GT 8 proteins are retaining enzymes based on the relative anomeric stereochemistry of the substrate and product in the reaction catalyzed. The non-catalytic N-terminal portion of the human UTG1 (HUGT1) has been shown to monitor the protein folding status and activate its glucosyltransferase activity. | ||
| Gene Ontology | |
|---|---|
| GO Term | Description |
| GO:0003980 | UDP-glucose:glycoprotein glucosyltransferase activity |
| GO:0006486 | protein glycosylation |
| GO:0016757 | transferase activity, transferring glycosyl groups |
| Annotations - NR Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| GenBank | AAG51883.1 | 0 | 20 | 1650 | 16 | 1674 | AC016162_4 putative UDP-glucose:glycoprotein glucosyltransferase; 101200-91134 [Arabidopsis thaliana] |
| DDBJ | BAD28382.1 | 0 | 30 | 1638 | 33 | 1617 | putative UDP-glucose:glycoprotein glucosyltransferase [Oryza sativa Japonica Group] |
| RefSeq | NP_177278.3 | 0 | 20 | 1650 | 16 | 1613 | EBS1 (EMS-mutagenized bri1 suppressor 1); UDP-glucose:glycoprotein glucosyltransferase/ transferase, transferring glycosyl groups / transferase, transferring hexosyl groups [Arabidopsis thaliana] |
| RefSeq | XP_002268972.1 | 0 | 27 | 1650 | 26 | 1611 | PREDICTED: similar to EBS1 (EMS-MUTAGENIZED BRI1 SUPPRESSOR 1); UDP-glucose:glycoprotein glucosyltransferase/ transferase, transferring glycosyl groups / transferase, transferring hexosyl groups [Vitis vinifera] |
| RefSeq | XP_002529534.1 | 0 | 2 | 1543 | 4 | 1499 | UDP-glucose glycoprotein:glucosyltransferase, putative [Ricinus communis] |
