Basic Information | |
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Species | Medicago truncatula |
Cazyme ID | Medtr2g099950.1 |
Family | GH32 |
Protein Properties | Length: 576 Molecular Weight: 64871 Isoelectric Point: 9.23 |
Chromosome | Chromosome/Scaffold: 2 Start: 31728798 End: 31732632 |
Description | Glycosyl hydrolases family 32 protein |
View CDS |
External Links |
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NCBI Taxonomy |
Plaza |
CAZyDB |
Signature Domain Download full data set without filtering | |||
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Family | Start | End | Evalue |
GH32 | 52 | 373 | 0 |
HFQPLKNWINDPNGPMRYGGLYHLFYQYNPKGAVWGNIVWAHSASKDLVNWTPLDHAIHPSQPSDIKGCWSGSATILPGGKPAILYTGIDPNNHQVQNIA IPKNMSDPLLREWKKSPKNPLMEPTVANKINASSFRDPTTSWLGKDGYWRVLIGSKVHRKGIAIMYKSKNFVDWFEAKHPLHSAEGTGMWECPDFFPVLN KNPLTIGLDTSVNGDDVRHVLKVSLDDTKHDHYLIGTYDTVKDVFVPDNGFENNQNVLRYDYGKYYASKTFFDDGKNRRILLGWANESSSVVDDVKKGWS GIHTIPRVIWLHKSGKQLVQWP |
Full Sequence |
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Protein Sequence Length: 576 Download |
MAISPILLIA LFSLIYGNYV IPIEATHHVY RTLESSSSYS SHHDQPYRTA YHFQPLKNWI 60 NDPNGPMRYG GLYHLFYQYN PKGAVWGNIV WAHSASKDLV NWTPLDHAIH PSQPSDIKGC 120 WSGSATILPG GKPAILYTGI DPNNHQVQNI AIPKNMSDPL LREWKKSPKN PLMEPTVANK 180 INASSFRDPT TSWLGKDGYW RVLIGSKVHR KGIAIMYKSK NFVDWFEAKH PLHSAEGTGM 240 WECPDFFPVL NKNPLTIGLD TSVNGDDVRH VLKVSLDDTK HDHYLIGTYD TVKDVFVPDN 300 GFENNQNVLR YDYGKYYASK TFFDDGKNRR ILLGWANESS SVVDDVKKGW SGIHTIPRVI 360 WLHKSGKQLV QWPVKELENL RMNPVNWPTK VIKGGELIPI TGVNSVQADV EISFEVKDFG 420 KAELLDKWID PQILCSQKGA SVKGGVGPFG LHVFASKGLQ EYTAVFFRIF RYQHKNLVLM 480 CSDQSRSSLN KENDMTTYGT FVDVDPLHEK LSLRTLIDHS VVESFGGEGR ACITARVYPT 540 LAIHDKALLY AFNNGNSAVK ITRLNAWSMK KAKIN* |
Functional Domains Download unfiltered results here | ||||||||
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Cdd ID | Domain | E-Value | Start | End | Length | Domain Description | ||
TIGR01322 | scrB_fam | 1.0e-52 | 46 | 539 | 509 | + sucrose-6-phosphate hydrolase. [Energy metabolism, Biosynthesis and degradation of polysaccharides]. | ||
COG1621 | SacC | 3.0e-68 | 44 | 540 | 517 | + Beta-fructosidases (levanase/invertase) [Carbohydrate transport and metabolism] | ||
cd08996 | GH32_B_Fructosidase | 5.0e-91 | 58 | 376 | 332 | + Glycosyl hydrolase family 32, beta-fructosidases. Glycosyl hydrolase family GH32 cleaves sucrose into fructose and glucose via beta-fructofuranosidase activity, producing invert sugar that is a mixture of dextrorotatory D-glucose and levorotatory D-fructose, thus named invertase (EC 3.2.1.26). This family also contains other fructofuranosidases such as inulinase (EC 3.2.1.7), exo-inulinase (EC 3.2.1.80), levanase (EC 3.2.1.65), and transfructosidases such sucrose:sucrose 1-fructosyltransferase (EC 2.4.1.99), fructan:fructan 1-fructosyltransferase (EC 2.4.1.100), sucrose:fructan 6-fructosyltransferase (EC 2.4.1.10), fructan:fructan 6G-fructosyltransferase (EC 2.4.1.243) and levan fructosyltransferases (EC 2.4.1.-). These retaining enzymes (i.e. they retain the configuration at anomeric carbon atom of the substrate) catalyze hydrolysis in two steps involving a covalent glycosyl enzyme intermediate: an aspartate located close to the N-terminus acts as the catalytic nucleophile and a glutamate acts as the general acid/base; a conserved aspartate residue in the Arg-Asp-Pro (RDP) motif stabilizes the transition state. These enzymes are predicted to display a 5-fold beta-propeller fold as found for GH43 and CH68. The breakdown of sucrose is widely used as a carbon or energy source by bacteria, fungi, and plants. Invertase is used commercially in the confectionery industry, since fructose has a sweeter taste than sucrose and a lower tendency to crystallize. A common structural feature of all these enzymes is a 5-bladed beta-propeller domain, similar to GH43, that contains the catalytic acid and catalytic base. A long V-shaped groove, partially enclosed at one end, forms a single extended substrate-binding surface across the face of the propeller. | ||
pfam00251 | Glyco_hydro_32N | 2.0e-142 | 52 | 373 | 330 | + Glycosyl hydrolases family 32 N-terminal domain. This domain corresponds to the N-terminal domain of glycosyl hydrolase family 32 which forms a five bladed beta propeller structure. | ||
smart00640 | Glyco_32 | 2.0e-178 | 52 | 529 | 488 | + Glycosyl hydrolases family 32. |
Annotations - NR Download unfiltered results here | |||||||
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Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
GenBank | AAC17166.1 | 0 | 24 | 573 | 21 | 573 | cell wall invertase [Pisum sativum] |
GenBank | ABD28503.1 | 0 | 22 | 575 | 22 | 572 | Sialidase [Medicago truncatula] |
EMBL | CAA84527.1 | 0 | 22 | 575 | 23 | 578 | cell wall invertase II; beta-furanofructosidase [Vicia faba var. minor] |
EMBL | CAD91338.1 | 0 | 1 | 575 | 1 | 564 | beta-fructofuranosidase [Glycine max] |
Swiss-Prot | Q43089 | 0 | 24 | 549 | 21 | 549 | INV1_PEA RecName: Full=Beta-fructofuranosidase, cell wall isozyme; AltName: Full=Sucrose hydrolase; AltName: Full=Acid invertase; Flags: Precursor |
Annotations - PDB Download unfiltered results here | |||||||
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Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
PDB | 2ac1_A | 0 | 44 | 575 | 5 | 541 | A Chain A, Crystal Structure Of A Cell-Wall Invertase From Arabidopsis Thaliana |
PDB | 2xqr_K | 0 | 44 | 575 | 1 | 537 | B Chain B, Crystal Structure Of Plant Cell Wall Invertase In Complex With A Specific Protein Inhibitor |
PDB | 2xqr_I | 0 | 44 | 575 | 1 | 537 | B Chain B, Crystal Structure Of Plant Cell Wall Invertase In Complex With A Specific Protein Inhibitor |
PDB | 2xqr_G | 0 | 44 | 575 | 1 | 537 | B Chain B, Crystal Structure Of Plant Cell Wall Invertase In Complex With A Specific Protein Inhibitor |
PDB | 2xqr_E | 0 | 44 | 575 | 1 | 537 | B Chain B, Crystal Structure Of Plant Cell Wall Invertase In Complex With A Specific Protein Inhibitor |