Basic Information | |
---|---|
Species | Medicago truncatula |
Cazyme ID | Medtr3g008530.1 |
Family | GH32 |
Protein Properties | Length: 518 Molecular Weight: 57884.2 Isoelectric Point: 4.5258 |
Chromosome | Chromosome/Scaffold: 3 Start: 1578699 End: 1581635 |
Description | Glycosyl hydrolases family 32 protein |
View CDS |
External Links |
---|
NCBI Taxonomy |
Plaza |
CAZyDB |
Signature Domain Download full data set without filtering | |||
---|---|---|---|
Family | Start | End | Evalue |
GH32 | 7 | 311 | 0 |
PLFHMGWYHLFYQYNPDSAVWGNISWGHAVSSDMIHWLYLPIAMEPDKWFDINGVWTGSATLLPDGEVIMLYTGDTDNYVQVQNLAHPANLSDPLLLDWV KYANNPILEPPPGIGSKDFRDPTTGWIGPDGKWRVLIGSKKGQTGLSLVYKTTNFINFELNDNYLHAVPGTGMWECVDFYPVSINGSNGLDTSVNGPHVK HVLKASLDDTRVDSYAIGTYFIENDTWIPDNPLEDVGIGLLLDYGIYYASKTFYDQVKKRRILWGWINETDAESDDLEKGWASLQTIPRTVLFDQKTGTN LLQWP |
Full Sequence |
---|
Protein Sequence Length: 518 Download |
MFVLVSPLFH MGWYHLFYQY NPDSAVWGNI SWGHAVSSDM IHWLYLPIAM EPDKWFDING 60 VWTGSATLLP DGEVIMLYTG DTDNYVQVQN LAHPANLSDP LLLDWVKYAN NPILEPPPGI 120 GSKDFRDPTT GWIGPDGKWR VLIGSKKGQT GLSLVYKTTN FINFELNDNY LHAVPGTGMW 180 ECVDFYPVSI NGSNGLDTSV NGPHVKHVLK ASLDDTRVDS YAIGTYFIEN DTWIPDNPLE 240 DVGIGLLLDY GIYYASKTFY DQVKKRRILW GWINETDAES DDLEKGWASL QTIPRTVLFD 300 QKTGTNLLQW PVEEVESLRL SSDEYAEVVV TPGSVVPLNI TQATQLDIFA EFEIESLTSK 360 ENISNDNIDC GRGSIDRSDF GPFGILAIAH DTLSEQTPIY FRLSNTSLGS STNLFCVDGT 420 RSSKAPDVEK RVYGSKVPVF SDEKLSMRVL VDHSIIESFA QGGRVVISGR VYPTEAIYGA 480 AKLFLFNNAT NINIKVSLKI WHLNSAFIRP FPFDQSQ* |
Functional Domains Download unfiltered results here | ||||||||
---|---|---|---|---|---|---|---|---|
Cdd ID | Domain | E-Value | Start | End | Length | Domain Description | ||
TIGR01322 | scrB_fam | 7.0e-41 | 12 | 473 | 486 | + sucrose-6-phosphate hydrolase. [Energy metabolism, Biosynthesis and degradation of polysaccharides]. | ||
COG1621 | SacC | 1.0e-56 | 7 | 475 | 490 | + Beta-fructosidases (levanase/invertase) [Carbohydrate transport and metabolism] | ||
cd08996 | GH32_B_Fructosidase | 3.0e-82 | 7 | 314 | 314 | + Glycosyl hydrolase family 32, beta-fructosidases. Glycosyl hydrolase family GH32 cleaves sucrose into fructose and glucose via beta-fructofuranosidase activity, producing invert sugar that is a mixture of dextrorotatory D-glucose and levorotatory D-fructose, thus named invertase (EC 3.2.1.26). This family also contains other fructofuranosidases such as inulinase (EC 3.2.1.7), exo-inulinase (EC 3.2.1.80), levanase (EC 3.2.1.65), and transfructosidases such sucrose:sucrose 1-fructosyltransferase (EC 2.4.1.99), fructan:fructan 1-fructosyltransferase (EC 2.4.1.100), sucrose:fructan 6-fructosyltransferase (EC 2.4.1.10), fructan:fructan 6G-fructosyltransferase (EC 2.4.1.243) and levan fructosyltransferases (EC 2.4.1.-). These retaining enzymes (i.e. they retain the configuration at anomeric carbon atom of the substrate) catalyze hydrolysis in two steps involving a covalent glycosyl enzyme intermediate: an aspartate located close to the N-terminus acts as the catalytic nucleophile and a glutamate acts as the general acid/base; a conserved aspartate residue in the Arg-Asp-Pro (RDP) motif stabilizes the transition state. These enzymes are predicted to display a 5-fold beta-propeller fold as found for GH43 and CH68. The breakdown of sucrose is widely used as a carbon or energy source by bacteria, fungi, and plants. Invertase is used commercially in the confectionery industry, since fructose has a sweeter taste than sucrose and a lower tendency to crystallize. A common structural feature of all these enzymes is a 5-bladed beta-propeller domain, similar to GH43, that contains the catalytic acid and catalytic base. A long V-shaped groove, partially enclosed at one end, forms a single extended substrate-binding surface across the face of the propeller. | ||
pfam00251 | Glyco_hydro_32N | 5.0e-134 | 7 | 311 | 313 | + Glycosyl hydrolases family 32 N-terminal domain. This domain corresponds to the N-terminal domain of glycosyl hydrolase family 32 which forms a five bladed beta propeller structure. | ||
smart00640 | Glyco_32 | 1.0e-166 | 7 | 463 | 467 | + Glycosyl hydrolases family 32. |
Annotations - NR Download unfiltered results here | |||||||
---|---|---|---|---|---|---|---|
Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
GenBank | ACJ04702.1 | 0 | 7 | 511 | 125 | 629 | invertase 2 [Cucumis melo] |
DDBJ | BAF34363.1 | 0 | 7 | 515 | 136 | 641 | beta-fructofuranosidase [Citrus sinensis] |
DDBJ | BAF35858.1 | 0 | 7 | 515 | 137 | 644 | Soluble acid invertase [Pyrus pyrifolia var. culta] |
DDBJ | BAG30919.1 | 0 | 7 | 515 | 137 | 644 | soluble acid invertase [Pyrus pyrifolia] |
RefSeq | XP_002510944.1 | 0 | 7 | 516 | 132 | 639 | Beta-fructofuranosidase, soluble isoenzyme I precursor, putative [Ricinus communis] |
Annotations - PDB Download unfiltered results here | |||||||
---|---|---|---|---|---|---|---|
Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
PDB | 3ugh_B | 0 | 7 | 511 | 37 | 544 | A Chain A, The Structure Of Endoglucanase From Termite, Nasutitermes Takasagoensis, At Ph 2.5. |
PDB | 3ugh_A | 0 | 7 | 511 | 37 | 544 | A Chain A, The Structure Of Endoglucanase From Termite, Nasutitermes Takasagoensis, At Ph 2.5. |
PDB | 3ugg_B | 0 | 7 | 511 | 37 | 544 | A Chain A, The Structure Of Endoglucanase From Termite, Nasutitermes Takasagoensis, At Ph 2.5. |
PDB | 3ugg_A | 0 | 7 | 511 | 37 | 544 | A Chain A, The Structure Of Endoglucanase From Termite, Nasutitermes Takasagoensis, At Ph 2.5. |
PDB | 3ugf_B | 0 | 7 | 511 | 37 | 544 | A Chain A, Crystal Structure Of A 6-Sst6-Sft From Pachysandra Terminalis |
EST Download unfiltered results here | ||||
---|---|---|---|---|
Hit | Length | Start | End | EValue |
FC906419 | 287 | 7 | 293 | 0 |
DY265807 | 287 | 7 | 293 | 0 |
DT554487 | 322 | 59 | 378 | 0 |
DY297064 | 312 | 73 | 384 | 0 |
DT550231 | 289 | 7 | 294 | 0 |
Sequence Alignments (This image is cropped. Click for full image.) |
---|