| Basic Information | |
|---|---|
| Species | Medicago truncatula |
| Cazyme ID | Medtr4g128590.1 |
| Family | GH16 |
| Protein Properties | Length: 279 Molecular Weight: 31190.1 Isoelectric Point: 7.4429 |
| Chromosome | Chromosome/Scaffold: 4 Start: 44971176 End: 44972269 |
| Description | Xyloglucan endotransglucosylase/hydrolase family protein |
| View CDS | |
| External Links |
|---|
| NCBI Taxonomy |
| Plaza |
| CAZyDB |
| Signature Domain Download full data set without filtering | |||
|---|---|---|---|
| Family | Start | End | Evalue |
| GH16 | 59 | 196 | 1.5e-23 |
| KIDMQLKLVPGNSAGTVTAYYLSSKGPTWDEIDYEFLGNVSGEPYILHTNVFSQGKGNREQQFYLWFDPAADFHTYSIIWNPQRIIFSVDGTPIREFKNS ETIGVPFPKNQPMRIYSSLWNADNWATRGGLVKTDWTK | |||
| Full Sequence |
|---|
| Protein Sequence Length: 279 Download |
| MVSSNDFNML ILIPLLVSSL MVMNAFAAGN LNQHFDITWG DGRAKILNNG ELLTLSLDKI 60 DMQLKLVPGN SAGTVTAYYL SSKGPTWDEI DYEFLGNVSG EPYILHTNVF SQGKGNREQQ 120 FYLWFDPAAD FHTYSIIWNP QRIIFSVDGT PIREFKNSET IGVPFPKNQP MRIYSSLWNA 180 DNWATRGGLV KTDWTKAPFT ASYRNFNAQA CIWSSGASSC GSTSSASKDS ARGSWLSQEL 240 DATGQGRLKW VQKNYMIYNY CKDTKRFPQG LPPECNHS* |
| Functional Domains Download unfiltered results here | ||||||
|---|---|---|---|---|---|---|
| Cdd ID | Domain | E-Value | Start | End | Length | Domain Description |
| pfam06955 | XET_C | 4.0e-22 | 235 | 275 | 44 | + Xyloglucan endo-transglycosylase (XET) C-terminus. This family represents the C-terminus (approximately 60 residues) of plant xyloglucan endo-transglycosylase (XET). Xyloglucan is the predominant hemicellulose in the cell walls of most dicotyledons. With cellulose, it forms a network that strengthens the cell wall. XET catalyzes the splitting of xyloglucan chains and the linking of the newly generated reducing end to the non-reducing end of another xyloglucan chain, thereby loosening the cell wall. Note that all family members contain the pfam00722 domain. |
| cd02183 | GH16_fungal_CRH1_transglycosylase | 2.0e-26 | 59 | 209 | 164 | + glycosylphosphatidylinositol-glucanosyltransferase. Group of fungal GH16 members related to Saccharomyces cerevisiae Crh1p. Chr1p and Crh2p are transglycosylases that are required for the linkage of chitin to beta(1-3)glucose branches of beta(1-6)glucan, an important step in the assembly of new cell wall. Both have been shown to be glycosylphosphatidylinositol (GPI)-anchored. A third homologous protein, Crr1p, functions in the formation of the spore wall. They belongs to the family 16 of glycosyl hydrolases that includes lichenase, xyloglucan endotransglycosylase (XET), beta-agarase, kappa-carrageenase, endo-beta-1,3-glucanase, endo-beta-1,3-1,4-glucanase, and endo-beta-galactosidase, all of which have a conserved jelly roll fold with a deep active site channel harboring the catalytic residues. |
| pfam00722 | Glyco_hydro_16 | 2.0e-68 | 32 | 196 | 183 | + Glycosyl hydrolases family 16. |
| PLN03161 | PLN03161 | 3.0e-104 | 11 | 275 | 281 | + Probable xyloglucan endotransglucosylase/hydrolase protein; Provisional |
| cd02176 | GH16_XET | 4.0e-136 | 26 | 275 | 267 | + Xyloglucan endotransglycosylase, member of glycosyl hydrolase family 16. Xyloglucan endotransglycosylases (XETs) cleave and religate xyloglucan polymers in plant cell walls via a transglycosylation mechanism. Xyloglucan is a soluble hemicellulose with a backbone of beta-1,4-linked glucose units, partially substituted with alpha-1,6-linked xylopyranose branches. It binds noncovalently to cellulose, cross-linking the adjacent cellulose microfibrils, giving it a key structural role as a matrix polymer. Therefore, XET plays an important role in all plant processes that require cell wall remodeling. |
| Gene Ontology | |
|---|---|
| GO Term | Description |
| GO:0004553 | hydrolase activity, hydrolyzing O-glycosyl compounds |
| GO:0005618 | cell wall |
| GO:0005975 | carbohydrate metabolic process |
| GO:0006073 | cellular glucan metabolic process |
| GO:0016762 | xyloglucan:xyloglucosyl transferase activity |
| Annotations - NR Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| EMBL | CAD87535.1 | 0 | 11 | 276 | 6 | 281 | putative xyloglucan endotransglycosylase [Cucumis sativus] |
| EMBL | CAN74784.1 | 0 | 10 | 277 | 13 | 292 | hypothetical protein [Vitis vinifera] |
| EMBL | CAN80250.1 | 0 | 15 | 275 | 6 | 281 | hypothetical protein [Vitis vinifera] |
| RefSeq | XP_002270182.1 | 0 | 1 | 275 | 1 | 290 | PREDICTED: hypothetical protein [Vitis vinifera] |
| RefSeq | XP_002318936.1 | 0 | 9 | 278 | 14 | 298 | predicted protein [Populus trichocarpa] |
| Annotations - PDB Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| PDB | 1un1_B | 0 | 38 | 277 | 24 | 274 | A Chain A, Raphanus Sativus Anionic Peroxidase. |
| PDB | 1un1_A | 0 | 38 | 277 | 24 | 274 | A Chain A, Raphanus Sativus Anionic Peroxidase. |
| PDB | 1umz_B | 0 | 38 | 277 | 24 | 274 | A Chain A, Xyloglucan Endotransglycosylase In Complex With The Xyloglucan Nonasaccharide Xllg. |
| PDB | 1umz_A | 0 | 38 | 277 | 24 | 274 | A Chain A, Xyloglucan Endotransglycosylase In Complex With The Xyloglucan Nonasaccharide Xllg. |
| PDB | 2uwb_B | 0 | 64 | 275 | 67 | 267 | A Chain A, Crystal Structure Of The Nasturtium Seedling Mutant Xyloglucanase Isoform Nxg1-Delta-Yniig |
