Basic Information | |
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Species | Medicago truncatula |
Cazyme ID | Medtr7g104080.1 |
Family | GH13 |
Protein Properties | Length: 566 Molecular Weight: 64078 Isoelectric Point: 6.9996 |
Chromosome | Chromosome/Scaffold: 7 Start: 33271910 End: 33282477 |
Description | starch branching enzyme 2.1 |
View CDS |
External Links |
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NCBI Taxonomy |
Plaza |
CAZyDB |
Signature Domain Download full data set without filtering | |||
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Family | Start | End | Evalue |
GH13 | 406 | 544 | 5.8e-28 |
LPRIKKLGYNAVQIMAIQEHSYYASFGYHVTNFFAPSSRFGTPEDLKSLIDRAHELGLLVLMDIVHSHASSNTLDGLNMFDGTDAHYFHSGSRGYHWMWD SRLFNYGSWEVLRYLLSNARWWLDEYKFDGFRFDGVTSM |
Full Sequence |
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Protein Sequence Length: 566 Download |
MVYTISGIRF PVVPSLNKSS LRGGDRRTSS SHSFFLKKNS SSFSKTSLYA KFSHDSETKS 60 STIAESDKVL IPEDQDNSAS VTDQLENPDI TSEDAQSFQN LEDLTMKDEN KYNVDEAASS 120 YREVGDDKGS VMPSSLVDVK TDAQAKKTSV HSDKKVKTDK PKVIPPPGAG QKIYEIDTFL 180 QAHRQHLDFR YGQYKKIREE IDKYEGGLDA FSRGYEKLGF TRSATGITYR EWAPGAKSAA 240 LVGDFNNWNP NADVMTRDDF GVWEIFLPNN ADGSPPIPHG SRVKIHMDTP SGIKDSIPAW 300 IKFSVQAPGE IPYNGIYYDP PEEEKYVFKH PQPKRPQSIR IYEAHVGMSS PVCTLYSLLH 360 QKVLLLCIIS GKMSRTKIVF LPTQATSWFI AEPKINTYAN FRDDVLPRIK KLGYNAVQIM 420 AIQEHSYYAS FGYHVTNFFA PSSRFGTPED LKSLIDRAHE LGLLVLMDIV HSHASSNTLD 480 GLNMFDGTDA HYFHSGSRGY HWMWDSRLFN YGSWEVLRYL LSNARWWLDE YKFDGFRFDG 540 VTSMMYTHHG LQFYSLMGLH TYTNA* |
Functional Domains Download unfiltered results here | ||||||||
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Cdd ID | Domain | E-Value | Start | End | Length | Domain Description | ||
PLN02960 | PLN02960 | 7.0e-12 | 181 | 267 | 93 | + alpha-amylase | ||
COG0296 | GlgB | 8.0e-76 | 215 | 551 | 345 | + 1,4-alpha-glucan branching enzyme [Carbohydrate transport and metabolism] | ||
PLN02960 | PLN02960 | 5.0e-91 | 275 | 564 | 292 | + alpha-amylase | ||
cd11321 | AmyAc_bac_euk_BE | 3.0e-134 | 322 | 562 | 241 | + Alpha amylase catalytic domain found in bacterial and eukaryotic branching enzymes. Branching enzymes (BEs) catalyze the formation of alpha-1,6 branch points in either glycogen or starch by cleavage of the alpha-1,4 glucosidic linkage yielding a non-reducing end oligosaccharide chain, and subsequent attachment to the alpha-1,6 position. By increasing the number of non-reducing ends, glycogen is more reactive to synthesis and digestion as well as being more soluble. This group includes bacterial and eukaryotic proteins. The Alpha-amylase family comprises the largest family of glycoside hydrolases (GH), with the majority of enzymes acting on starch, glycogen, and related oligo- and polysaccharides. These proteins catalyze the transformation of alpha-1,4 and alpha-1,6 glucosidic linkages with retention of the anomeric center. The protein is described as having 3 domains: A, B, C. A is a (beta/alpha) 8-barrel; B is a loop between the beta 3 strand and alpha 3 helix of A; C is the C-terminal extension characterized by a Greek key. The majority of the enzymes have an active site cleft found between domains A and B where a triad of catalytic residues (Asp, Glu and Asp) performs catalysis. Other members of this family have lost the catalytic activity as in the case of the human 4F2hc, or only have 2 residues that serve as the catalytic nucleophile and the acid/base, such as Thermus A4 beta-galactosidase with 2 Glu residues (GH42) and human alpha-galactosidase with 2 Asp residues (GH31). The family members are quite extensive and include: alpha amylase, maltosyltransferase, cyclodextrin glycotransferase, maltogenic amylase, neopullulanase, isoamylase, 1,4-alpha-D-glucan maltotetrahydrolase, 4-alpha-glucotransferase, oligo-1,6-glucosidase, amylosucrase, sucrose phosphorylase, and amylomaltase. | ||
PLN02447 | PLN02447 | 0 | 124 | 552 | 433 | + 1,4-alpha-glucan-branching enzyme |
Gene Ontology | |
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GO Term | Description |
GO:0003824 | catalytic activity |
GO:0004553 | hydrolase activity, hydrolyzing O-glycosyl compounds |
GO:0005975 | carbohydrate metabolic process |
GO:0043169 | cation binding |
Annotations - NR Download unfiltered results here | |||||||
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Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
GenBank | AAT76444.1 | 0 | 1 | 552 | 1 | 491 | starch branching enzyme II [Vigna radiata] |
GenBank | ABO31358.1 | 0 | 1 | 552 | 1 | 501 | starch branching enzyme II-1 [Malus x domestica] |
DDBJ | BAA82348.2 | 0 | 1 | 552 | 1 | 506 | starch branching enzyme [Phaseolus vulgaris] |
EMBL | CAA56319.1 | 0 | 1 | 552 | 1 | 507 | starch branching enzyme I [Pisum sativum] |
EMBL | CBI30261.1 | 0 | 1 | 552 | 1 | 513 | unnamed protein product [Vitis vinifera] |
Annotations - PDB Download unfiltered results here | |||||||
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Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
PDB | 3amk_A | 0 | 173 | 552 | 13 | 357 | A Chain A, Structure Of The Starch Branching Enzyme I (Bei) From Oryza Sativa L |
PDB | 3vu2_B | 0 | 173 | 552 | 13 | 357 | A Chain A, Structure Of The Starch Branching Enzyme I (bei) Complexed With Maltopentaose From Oryza Sativa L |
PDB | 3vu2_A | 0 | 173 | 552 | 13 | 357 | A Chain A, Structure Of The Starch Branching Enzyme I (bei) Complexed With Maltopentaose From Oryza Sativa L |
PDB | 3aml_A | 0 | 173 | 552 | 13 | 357 | A Chain A, Structure Of The Starch Branching Enzyme I (Bei) From Oryza Sativa L |
PDB | 3k1d_A | 2e-29 | 225 | 546 | 136 | 409 | A Chain A, Crystal Structure Of Glycogen Branching Enzyme Synonym: 1,4- Glucan:1,4-Alpha-D-Glucan 6-Glucosyl-Transferase From Mycob Tuberculosis H3 |
EST Download unfiltered results here | ||||
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Hit | Length | Start | End | EValue |
HO794536 | 386 | 167 | 552 | 0 |
HO777638 | 330 | 223 | 552 | 0 |
GW715806 | 338 | 159 | 496 | 0 |
HO777638 | 47 | 176 | 222 | 0.00000000004 |
HO794536 | 82 | 95 | 172 | 0.000000007 |
Sequence Alignments (This image is cropped. Click for full image.) |
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