| Basic Information | |
|---|---|
| Species | Panicum virgatum |
| Cazyme ID | Pavirv00005120m |
| Family | GH32 |
| Protein Properties | Length: 587 Molecular Weight: 64599.5 Isoelectric Point: 6.172 |
| Chromosome | Chromosome/Scaffold: 018375 Start: 6304 End: 9672 |
| Description | beta-fructofuranosidase 5 |
| View CDS | |
| External Links |
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| CAZyDB |
| Signature Domain Download full data set without filtering | |||
|---|---|---|---|
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| Family | Start | End | Evalue |
| GH32 | 42 | 360 | 0 |
| HFQPAKNWQNGPMYYNGMYHFFYQYNPHGALWDMGNLSWGHSVSGDLVNWAALDTALDPTAPFDINGCWSGSSTILPGGTPAILYTGIDANNEQVQNVAF PKNPADPLLREWVKPSYNPVIALPADVPGDKFRDPSTAWLGRDGLWRIAVSAEVSGVASTLVYRSADFRRWEQNAAPLHSSRAAGMVECPDLFPVKQHGK DGLDTSANGPGVSHVLKLSVMDTLQDYYMVGRYDDAADTFAPAEPERGDDVRNWRRFDYGHVYASKSFFDARKNRRVLWSWANESDSQADDVARGWSGVQ TVPRKVWLDKDGKQLRQWP | |||
| Full Sequence |
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| Protein Sequence Length: 587 Download |
| MAGLPLAAVA FHLCLLASSS AALRLAPETT GEGRHVGRTA YHFQPAKNWQ NGPMYYNGMY 60 HFFYQYNPHG ALWDMGNLSW GHSVSGDLVN WAALDTALDP TAPFDINGCW SGSSTILPGG 120 TPAILYTGID ANNEQVQNVA FPKNPADPLL REWVKPSYNP VIALPADVPG DKFRDPSTAW 180 LGRDGLWRIA VSAEVSGVAS TLVYRSADFR RWEQNAAPLH SSRAAGMVEC PDLFPVKQHG 240 KDGLDTSANG PGVSHVLKLS VMDTLQDYYM VGRYDDAADT FAPAEPERGD DVRNWRRFDY 300 GHVYASKSFF DARKNRRVLW SWANESDSQA DDVARGWSGV QTVPRKVWLD KDGKQLRQWP 360 IEEIETLRRK RVGLRRDSDT VLNAGAMNEI VGVAGSQADV EVAFKIPRLE AAESLDPNWL 420 LDPQKLCGEK GASVPSGVGP FGLIVMASGD LPEHTAVFFR VFRHHGDYKL LMCTDLTKSS 480 TRAGVYKPPY GGFVDIDIKE HKTIKLRTLI DHSVVESYGA EGRACITARV YPEHAQTSNS 540 HMFVFNNGTG TVKVTKLEAW ELATATVNVG DEGFIVSESK DETESY* |
| Functional Domains Download unfiltered results here | ||||||||
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| Cdd ID | Domain | E-Value | Start | End | Length | Domain Description | ||
| COG1621 | SacC | 0.002 | 481 | 556 | 76 | + Beta-fructosidases (levanase/invertase) [Carbohydrate transport and metabolism] | ||
| COG1621 | SacC | 3.0e-53 | 38 | 369 | 350 | + Beta-fructosidases (levanase/invertase) [Carbohydrate transport and metabolism] | ||
| cd08996 | GH32_B_Fructosidase | 2.0e-83 | 51 | 363 | 319 | + Glycosyl hydrolase family 32, beta-fructosidases. Glycosyl hydrolase family GH32 cleaves sucrose into fructose and glucose via beta-fructofuranosidase activity, producing invert sugar that is a mixture of dextrorotatory D-glucose and levorotatory D-fructose, thus named invertase (EC 3.2.1.26). This family also contains other fructofuranosidases such as inulinase (EC 3.2.1.7), exo-inulinase (EC 3.2.1.80), levanase (EC 3.2.1.65), and transfructosidases such sucrose:sucrose 1-fructosyltransferase (EC 2.4.1.99), fructan:fructan 1-fructosyltransferase (EC 2.4.1.100), sucrose:fructan 6-fructosyltransferase (EC 2.4.1.10), fructan:fructan 6G-fructosyltransferase (EC 2.4.1.243) and levan fructosyltransferases (EC 2.4.1.-). These retaining enzymes (i.e. they retain the configuration at anomeric carbon atom of the substrate) catalyze hydrolysis in two steps involving a covalent glycosyl enzyme intermediate: an aspartate located close to the N-terminus acts as the catalytic nucleophile and a glutamate acts as the general acid/base; a conserved aspartate residue in the Arg-Asp-Pro (RDP) motif stabilizes the transition state. These enzymes are predicted to display a 5-fold beta-propeller fold as found for GH43 and CH68. The breakdown of sucrose is widely used as a carbon or energy source by bacteria, fungi, and plants. Invertase is used commercially in the confectionery industry, since fructose has a sweeter taste than sucrose and a lower tendency to crystallize. A common structural feature of all these enzymes is a 5-bladed beta-propeller domain, similar to GH43, that contains the catalytic acid and catalytic base. A long V-shaped groove, partially enclosed at one end, forms a single extended substrate-binding surface across the face of the propeller. | ||
| pfam00251 | Glyco_hydro_32N | 8.0e-128 | 42 | 360 | 330 | + Glycosyl hydrolases family 32 N-terminal domain. This domain corresponds to the N-terminal domain of glycosyl hydrolase family 32 which forms a five bladed beta propeller structure. | ||
| smart00640 | Glyco_32 | 2.0e-156 | 42 | 522 | 494 | + Glycosyl hydrolases family 32. | ||
| Annotations - NR Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| Swiss-Prot | A2YZ01 | 0 | 3 | 577 | 5 | 584 | INV7_ORYSI RecName: Full=Beta-fructofuranosidase, insoluble isoenzyme 7; AltName: Full=Sucrose hydrolase 7; AltName: Full=Invertase 7; AltName: Full=Cell wall beta-fructosidase 7; AltName: Full=OsCIN7; Flags: Precursor |
| GenBank | AAD38399.1 | 0 | 3 | 577 | 5 | 584 | AF155121_1 apoplastic invertase [Oryza sativa Indica Group] |
| DDBJ | BAD23559.1 | 0 | 3 | 577 | 5 | 583 | putative apoplastic invertase [Oryza sativa Japonica Group] |
| RefSeq | NP_001062691.1 | 0 | 3 | 577 | 5 | 584 | Os09g0255000 [Oryza sativa (japonica cultivar-group)] |
| RefSeq | XP_002448713.1 | 0 | 1 | 586 | 1 | 599 | hypothetical protein SORBIDRAFT_06g031930 [Sorghum bicolor] |
| Annotations - PDB Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| PDB | 2ac1_A | 0 | 38 | 568 | 9 | 541 | A Chain A, Crystal Structure Of A Cell-Wall Invertase From Arabidopsis Thaliana |
| PDB | 2qqw_A | 0 | 38 | 568 | 5 | 537 | A Chain A, Crystal Structure Of A Cell-Wall Invertase (D23a) From Arabidopsis Thaliana In Complex With Sucrose |
| PDB | 2xqr_K | 0 | 38 | 568 | 5 | 537 | B Chain B, Crystal Structure Of Plant Cell Wall Invertase In Complex With A Specific Protein Inhibitor |
| PDB | 2xqr_I | 0 | 38 | 568 | 5 | 537 | B Chain B, Crystal Structure Of Plant Cell Wall Invertase In Complex With A Specific Protein Inhibitor |
| PDB | 2xqr_G | 0 | 38 | 568 | 5 | 537 | B Chain B, Crystal Structure Of Plant Cell Wall Invertase In Complex With A Specific Protein Inhibitor |
| Metabolic Pathways | |||
|---|---|---|---|
| Pathway Name | Reaction | EC | Protein Name |
| sucrose degradation III | RXN-1461 | EC-3.2.1.26 | β-fructofuranosidase |
