| Basic Information | |
|---|---|
| Species | Panicum virgatum |
| Cazyme ID | Pavirv00005372m |
| Family | GT68 |
| Protein Properties | Length: 561 Molecular Weight: 62147.4 Isoelectric Point: 7.6783 |
| Chromosome | Chromosome/Scaffold: 018069 Start: 417 End: 4643 |
| Description | O-fucosyltransferase family protein |
| View CDS | |
| External Links |
|---|
| CAZyDB |
| Signature Domain Download full data set without filtering | |||
|---|---|---|---|
 | |||
| Family | Start | End | Evalue |
| GT68 | 244 | 537 | 0 |
| TLVVPSQKVDYQYERVLDINHINDCIGRKVVITYEEFVEKRKKVSIDQFICYIASPPCFLDEDHIKKLKGLGISLGKIEAAWLEDAKLKEPKKRYVGDIT PKFSTDAEVLAIGDMFYADIEDEWVNQPGGPLAHKCKTLVQPSRLIMLTAQRFVQTFLGGNYIALHFRRHGFLKFCNVKKESCFFPIPQAAECILRIVEK ANAPVIYLSTDAADSETNLLQSLVVYNDRQVPLVKRPEHHSSEKWDALLYRNHIGGDGQVEAMLDKTICALSNVFIGSSGSTFTEDIFRLRRGW | |||
| Full Sequence |
|---|
| Protein Sequence Length: 561 Download |
| MDRDPSVSDE DDDLETLVPQ NHTKPPSPSS RSRSPPSSFG VAALRPALPS AAASLGRALW 60 SRRYLVLLVS LPLLALVLFL SLGGASSLRL PASIRLPFAA PAADPAASRM REAELRALYL 120 LRSQRSGLLS LFNRTAAPTN GSASISPPDL QAALESQIKI NREIQAALLS AHRSGAGNAT 180 EDGLDLDLHA AGCRRRELPA DRRTIEWNPK KDRFLLAICL SGQMSNHLIC LEKHMFMAAL 240 LGRTLVVPSQ KVDYQYERVL DINHINDCIG RKVVITYEEF VEKRKKVSID QFICYIASPP 300 CFLDEDHIKK LKGLGISLGK IEAAWLEDAK LKEPKKRYVG DITPKFSTDA EVLAIGDMFY 360 ADIEDEWVNQ PGGPLAHKCK TLVQPSRLIM LTAQRFVQTF LGGNYIALHF RRHGFLKFCN 420 VKKESCFFPI PQAAECILRI VEKANAPVIY LSTDAADSET NLLQSLVVYN DRQVPLVKRP 480 EHHSSEKWDA LLYRNHIGGD GQVEAMLDKT ICALSNVFIG SSGSTFTEDI FRLRRGWGSA 540 SHCDEYLCQG ELPNYIAEQD * |
| Functional Domains Download unfiltered results here | ||||||||
|---|---|---|---|---|---|---|---|---|
 | ||||||||
| Cdd ID | Domain | E-Value | Start | End | Length | Domain Description | ||
| cd11302 | O-FucT-1 | 0.0002 | 356 | 535 | 226 | + GDP-fucose protein O-fucosyltransferase 1. The protein O-fucosyltransferase 1 (Ofut1 or O-FucT-1) adds O-fucose to EGF (epidermal growth factor-like) repeats. The O-fucsosylation of the Notch receptor signaling protein is dependent on this enzyme, which requires GDP-fucose as a substrate. O-fucose residues added to the target of O-FucT-1 may be further elongated by other glycosyltransferases. On top of O-fucosylation, O-FucT-1 may have other functions such as the regulation of the Notch receptor exit from the ER. Six highly conserved cysteines are present in O-FucT-1, which is a soluble ER protein, as well as a DXD-like motif (ERD), conserved in mammals, Drosophila, and C. elegans. Both features are characteristic of several glycosyltransferase families. The membrane-bound pre-protein is released by proteolysis and, as for most glycosyltransferases, is strongly activated by manganese. O-FucT-1 is similar to family 1 glycosyltransferases (GT1). | ||
| cd11296 | O-FucT_like | 3.0e-5 | 214 | 257 | 44 | + GDP-fucose protein O-fucosyltransferase and related proteins. O-fucosyltransferase-like proteins are GDP-fucose dependent enzymes with similarities to the family 1 glycosyltransferases (GT1). They are soluble ER proteins that may be proteolytically cleaved from a membrane-associated preprotein, and are involved in the O-fucosylation of protein substrates, the core fucosylation of growth factor receptors, and other processes. | ||
| pfam10250 | O-FucT | 6.0e-8 | 238 | 535 | 350 | + GDP-fucose protein O-fucosyltransferase. This is a family of conserved proteins representing the enzyme responsible for adding O-fucose to EGF (epidermal growth factor-like) repeats. Six highly conserved cysteines are present in O-FucT-1 as well as a DXD-like motif (ERD), conserved in mammals, Drosophila, and C. elegans. Both features are characteristic of several glycosyltransferase families. The enzyme is a membrane-bound protein released by proteolysis and, as for most glycosyltransferases, is strongly activated by manganese. | ||
| cd11298 | O-FucT-2 | 2.0e-12 | 387 | 534 | 148 | + GDP-fucose protein O-fucosyltransferase 2. O-FucT-2 adds O-fucose to thrombospondin type 1 repeats (TSRs), and appears conserved in bilateria. The O-fucosylation of TSRs appears to play a role in regulating secretion of metalloproteases of the ADAMTS superfamily. O-fucosyltransferase-like proteins are GDP-fucose dependent enzymes with similarities to the family 1 glycosyltransferases (GT1). They are soluble ER proteins that may be proteolytically cleaved from a membrane-associated preprotein, and are involved in the O-fucosylation of protein substrates, the core fucosylation of growth factor receptors, and other processes. | ||
| cd11296 | O-FucT_like | 2.0e-19 | 375 | 535 | 171 | + GDP-fucose protein O-fucosyltransferase and related proteins. O-fucosyltransferase-like proteins are GDP-fucose dependent enzymes with similarities to the family 1 glycosyltransferases (GT1). They are soluble ER proteins that may be proteolytically cleaved from a membrane-associated preprotein, and are involved in the O-fucosylation of protein substrates, the core fucosylation of growth factor receptors, and other processes. | ||
| Annotations - NR Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| RefSeq | NP_001060060.1 | 0 | 1 | 560 | 1 | 557 | Os07g0572600 [Oryza sativa (japonica cultivar-group)] |
| RefSeq | NP_173170.2 | 0 | 8 | 560 | 6 | 564 | unknown protein [Arabidopsis thaliana] |
| RefSeq | XP_002264087.1 | 0 | 1 | 560 | 1 | 559 | PREDICTED: hypothetical protein [Vitis vinifera] |
| RefSeq | XP_002303337.1 | 0 | 102 | 560 | 62 | 527 | predicted protein [Populus trichocarpa] |
| RefSeq | XP_002460894.1 | 0 | 1 | 560 | 1 | 562 | hypothetical protein SORBIDRAFT_02g037060 [Sorghum bicolor] |
| Annotations - PDB Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| PDB | 4ap5_B | 0.003 | 401 | 534 | 263 | 376 | A Chain A, Crystal Structure Of Human Pofut2 |
| PDB | 4ap5_A | 0.003 | 401 | 534 | 263 | 376 | A Chain A, Crystal Structure Of Human Pofut2 |
| PDB | 4ap6_D | 0.003 | 401 | 527 | 277 | 383 | A Chain A, Crystal Structure Of Human Pofut2 E54a Mutant In Complex With Gdp- Fucose |
| PDB | 4ap6_C | 0.003 | 401 | 527 | 277 | 383 | A Chain A, Crystal Structure Of Human Pofut2 E54a Mutant In Complex With Gdp- Fucose |
| PDB | 4ap6_B | 0.003 | 401 | 527 | 277 | 383 | A Chain A, Crystal Structure Of Human Pofut2 E54a Mutant In Complex With Gdp- Fucose |
