| Basic Information | |
|---|---|
| Species | Panicum virgatum |
| Cazyme ID | Pavirv00016669m |
| Family | GH32 |
| Protein Properties | Length: 661 Molecular Weight: 71775.7 Isoelectric Point: 6.2942 |
| Chromosome | Chromosome/Scaffold: 062981 Start: 482 End: 3549 |
| Description | Glycosyl hydrolases family 32 protein |
| View CDS | |
| External Links |
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| CAZyDB |
| Signature Domain Download full data set without filtering | |||
|---|---|---|---|
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| Family | Start | End | Evalue |
| GH32 | 124 | 454 | 0 |
| HFQPQKNWMNDPNGPVYYKGWYHLFYQYNPDGAIWGNKIAWGHAVSRDLVHWRHLPLAMVPDQWYDINGVWTGSATTLPDGRLAMLYTGSTNESVQVQCL AVPADPADPLLTNWTKYDGNPVLLPPPAIGPKDFRDPTTAWFDPSDRAWRVVIGSKDPHHAGIAVTYRTKDLVHFDLLPGLLHRVEGTGMWECIDFYPVG TKPSNQHNGIDMSDAISENGVAGDVVHVMKASMDDDRHDYYALGRYDAAANAWTPLDPASDVGIGLRYDWGKFYASKTFYDPAKRRRVLWGWVGETDSER ADVAKGWASLQSLPRTVLLDTKTGANLLQWP | |||
| Full Sequence |
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| Protein Sequence Length: 661 Download |
| METWDSVPST TPLPYAYSPL PAADAASAEV SGAGRANGRR RPLCAAALVL SAALLLAVAA 60 LAGVRIAPSR PITVGVGETT TAARSRGPAA GVSEKTSGAQ GMLRGGNAFP WSNAMLQWQR 120 TGFHFQPQKN WMNDPNGPVY YKGWYHLFYQ YNPDGAIWGN KIAWGHAVSR DLVHWRHLPL 180 AMVPDQWYDI NGVWTGSATT LPDGRLAMLY TGSTNESVQV QCLAVPADPA DPLLTNWTKY 240 DGNPVLLPPP AIGPKDFRDP TTAWFDPSDR AWRVVIGSKD PHHAGIAVTY RTKDLVHFDL 300 LPGLLHRVEG TGMWECIDFY PVGTKPSNQH NGIDMSDAIS ENGVAGDVVH VMKASMDDDR 360 HDYYALGRYD AAANAWTPLD PASDVGIGLR YDWGKFYASK TFYDPAKRRR VLWGWVGETD 420 SERADVAKGW ASLQSLPRTV LLDTKTGANL LQWPVEEVDA LRTNSTDLSG ITVDHGSVFP 480 LSLRRATQLD IVAEFELDRR AVMALNEADV GYNCSTSGGA ASRGALGPFG LLVLADKHLH 540 EQTAVYFYVA KGLDGALTTH FCQDESRSSS ANDIVKRVVG SAVPVLDGED TLSLRVLVDH 600 SIVESFAQGG RSTATSRVYP TEAIYANAGV YLFNNATAAR VTAKKLVVHE MDSSYNHDYL 660 * |
| Functional Domains Download unfiltered results here | ||||||||
|---|---|---|---|---|---|---|---|---|
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| Cdd ID | Domain | E-Value | Start | End | Length | Domain Description | ||
| TIGR01322 | scrB_fam | 5.0e-62 | 120 | 620 | 521 | + sucrose-6-phosphate hydrolase. [Energy metabolism, Biosynthesis and degradation of polysaccharides]. | ||
| COG1621 | SacC | 4.0e-76 | 120 | 636 | 541 | + Beta-fructosidases (levanase/invertase) [Carbohydrate transport and metabolism] | ||
| cd08996 | GH32_B_Fructosidase | 4.0e-95 | 130 | 457 | 333 | + Glycosyl hydrolase family 32, beta-fructosidases. Glycosyl hydrolase family GH32 cleaves sucrose into fructose and glucose via beta-fructofuranosidase activity, producing invert sugar that is a mixture of dextrorotatory D-glucose and levorotatory D-fructose, thus named invertase (EC 3.2.1.26). This family also contains other fructofuranosidases such as inulinase (EC 3.2.1.7), exo-inulinase (EC 3.2.1.80), levanase (EC 3.2.1.65), and transfructosidases such sucrose:sucrose 1-fructosyltransferase (EC 2.4.1.99), fructan:fructan 1-fructosyltransferase (EC 2.4.1.100), sucrose:fructan 6-fructosyltransferase (EC 2.4.1.10), fructan:fructan 6G-fructosyltransferase (EC 2.4.1.243) and levan fructosyltransferases (EC 2.4.1.-). These retaining enzymes (i.e. they retain the configuration at anomeric carbon atom of the substrate) catalyze hydrolysis in two steps involving a covalent glycosyl enzyme intermediate: an aspartate located close to the N-terminus acts as the catalytic nucleophile and a glutamate acts as the general acid/base; a conserved aspartate residue in the Arg-Asp-Pro (RDP) motif stabilizes the transition state. These enzymes are predicted to display a 5-fold beta-propeller fold as found for GH43 and CH68. The breakdown of sucrose is widely used as a carbon or energy source by bacteria, fungi, and plants. Invertase is used commercially in the confectionery industry, since fructose has a sweeter taste than sucrose and a lower tendency to crystallize. A common structural feature of all these enzymes is a 5-bladed beta-propeller domain, similar to GH43, that contains the catalytic acid and catalytic base. A long V-shaped groove, partially enclosed at one end, forms a single extended substrate-binding surface across the face of the propeller. | ||
| pfam00251 | Glyco_hydro_32N | 3.0e-157 | 124 | 454 | 338 | + Glycosyl hydrolases family 32 N-terminal domain. This domain corresponds to the N-terminal domain of glycosyl hydrolase family 32 which forms a five bladed beta propeller structure. | ||
| smart00640 | Glyco_32 | 0 | 124 | 610 | 496 | + Glycosyl hydrolases family 32. | ||
| Gene Ontology | |
|---|---|
| GO Term | Description |
| GO:0004564 | beta-fructofuranosidase activity |
| GO:0004575 | sucrose alpha-glucosidase activity |
| Annotations - NR Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| GenBank | AAC16654.1 | 0 | 105 | 660 | 4 | 563 | soluble acid invertase [Saccharum robustum] |
| GenBank | AAC16655.1 | 0 | 105 | 660 | 4 | 563 | soluble acid invertase [Saccharum officinarum] |
| GenBank | AAP59436.1 | 0 | 79 | 660 | 41 | 636 | soluble acid invertase [Saccharum hybrid cultivar Pindar] |
| GenBank | ABB77251.1 | 0 | 1 | 660 | 1 | 657 | vacuolar invertase BObetaFRUCT3 [Bambusa oldhamii] |
| GenBank | ACG30542.1 | 0 | 1 | 660 | 1 | 667 | beta-fructofuranosidase 1 precursor [Zea mays] |
| Annotations - PDB Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| PDB | 3ugh_B | 0 | 109 | 655 | 8 | 540 | A Chain A, Crystal Structure Of Alpha-Galactosidase I From Mortierella Vinacea |
| PDB | 3ugh_A | 0 | 109 | 655 | 8 | 540 | A Chain A, Crystal Structure Of Alpha-Galactosidase I From Mortierella Vinacea |
| PDB | 3ugg_B | 0 | 109 | 655 | 8 | 540 | A Chain A, Crystal Structure Of Alpha-Galactosidase I From Mortierella Vinacea |
| PDB | 3ugg_A | 0 | 109 | 655 | 8 | 540 | A Chain A, Crystal Structure Of Alpha-Galactosidase I From Mortierella Vinacea |
| PDB | 3ugf_B | 0 | 109 | 655 | 8 | 540 | A Chain A, Crystal Structure Of A 6-Sst6-Sft From Pachysandra Terminalis |
| Metabolic Pathways | |||
|---|---|---|---|
| Pathway Name | Reaction | EC | Protein Name |
| fructan biosynthesis | RXN-1781 | EC-2.4.1.99 | sucrose:sucrose fructosyltransferase |
| fructan biosynthesis | RXN-1801 | EC-2.4.1.100 | 2,1-fructan:2,1-fructan 1-fructosyltransferase |
| fructan biosynthesis | RXN-1803 | EC-2.4.1.10 | levansucrase |
| fructan biosynthesis | RXN-1804 | EC-2.4.1.10 | levansucrase |
| sucrose degradation III | RXN-1461 | EC-3.2.1.26 | β-fructofuranosidase |
