Basic Information | |
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Species | Panicum virgatum |
Cazyme ID | Pavirv00030888m |
Family | CBM45 |
Protein Properties | Length: 869 Molecular Weight: 97129.2 Isoelectric Point: 6.0832 |
Chromosome | Chromosome/Scaffold: 007993 Start: 2999 End: 13475 |
Description | alpha-amylase-like 3 |
View CDS |
External Links |
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CAZyDB |
Signature Domain Download full data set without filtering | |||
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Family | Start | End | Evalue |
CBM45 | 304 | 382 | 9.4e-21 |
LHWGVCRDNNMTWEILPEPHPPTTRIFRQKALQTLLQAKADGRGNSLSFLLDAQYSGLFFVLKLDEYTWLRNLENGSDF | |||
CBM45 | 120 | 203 | 2.6e-27 |
LHWGVSYDGEHGSEWDQPPSEMRPAGSVPIKDYAIETPLEILPNSEGWYEVQIKFDKDTPIAAINFVLKEEETGAWFQHKGRDF | |||
GH13 | 502 | 788 | 8.7e-37 |
KELSSLGFTIVWSPPPTDSVSPEGYMPRDLYNLNSRYGTMDELKELVKIFHEADIKVLGDAVLNHRCAQFQNNNGVWNIFGGRMNWDDRAVVADDPHFQG RGNKSSGDNFHAAPNIDHSQEFVRNDLKEWLCWMRKEVGYDGWRLDFVRGFWGGYVKDYLEASEPYFAVGEYWDSLSYTYGEMDYNQDAHRQRIVDWINA TNGTAGAFDVTTKGILHAALERSEYWRLSDEKGKPPGVLGWWPSRAVTFIENHDTGSTQGHWRFPYGMELQGYAYILTHPGTPAVFY |
Full Sequence |
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Protein Sequence Length: 869 Download |
MSVGSGCIPA IPGVAPQPRA GLLGGAFLQV AAARPRVGRC RATQHGRVRL GGRVVARAGA 60 AETPVAGADE GAGAVFSEKF PLRRCQTVEG KASVRVDAEP DGDGKCKVVV GCDVAGKWIL 120 HWGVSYDGEH GSEWDQPPSE MRPAGSVPIK DYAIETPLEI LPNSEGWYEV QIKFDKDTPI 180 AAINFVLKEE ETGAWFQHKG RDFRIPLRGS FDGGVPFGAN QDIGVWPGDL GHLKKHEGSN 240 AQPQETIPGG TNLSGKHISG FYQEFQIIKS EYTQNFVTVT VKRDNETHKR LVEFDTDIPG 300 EVILHWGVCR DNNMTWEILP EPHPPTTRIF RQKALQTLLQ AKADGRGNSL SFLLDAQYSG 360 LFFVLKLDEY TWLRNLENGS DFFISLTTME QRGSTQDLAD GIISGIRNLV VGLSSRRGQR 420 AKNKVLQEDI LQEIERLAAE AYSIFRSPTI DSVDASVDLD DPSIAKPACS GTGSGFEILC 480 QGFNWESHKS GKWYVELGKK AKELSSLGFT IVWSPPPTDS VSPEGYMPRD LYNLNSRYGT 540 MDELKELVKI FHEADIKVLG DAVLNHRCAQ FQNNNGVWNI FGGRMNWDDR AVVADDPHFQ 600 GRGNKSSGDN FHAAPNIDHS QEFVRNDLKE WLCWMRKEVG YDGWRLDFVR GFWGGYVKDY 660 LEASEPYFAV GEYWDSLSYT YGEMDYNQDA HRQRIVDWIN ATNGTAGAFD VTTKGILHAA 720 LERSEYWRLS DEKGKPPGVL GWWPSRAVTF IENHDTGSTQ GHWRFPYGME LQGYAYILTH 780 PGTPAVFYDH IFSHLQPEIA KFINIRQRQK IHCRSKIKIL KAERSLYAAE IDEKLIMKIG 840 SEHFEPSGTQ NWIVAAEGQE YKIWEVSS* |
Functional Domains Download unfiltered results here | ||||||||
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Cdd ID | Domain | E-Value | Start | End | Length | Domain Description | ||
PRK09441 | PRK09441 | 6.0e-50 | 477 | 808 | 420 | + cytoplasmic alpha-amylase; Reviewed | ||
PLN00196 | PLN00196 | 1.0e-133 | 477 | 865 | 406 | + alpha-amylase; Provisional | ||
cd11314 | AmyAc_arch_bac_plant_AmyA | 7.0e-157 | 478 | 817 | 343 | + Alpha amylase catalytic domain found in archaeal, bacterial, and plant Alpha-amylases (also called 1,4-alpha-D-glucan-4-glucanohydrolase). AmyA (EC 3.2.1.1) catalyzes the hydrolysis of alpha-(1,4) glycosidic linkages of glycogen, starch, related polysaccharides, and some oligosaccharides. This group includes AmyA from bacteria, archaea, water fleas, and plants. The Alpha-amylase family comprises the largest family of glycoside hydrolases (GH), with the majority of enzymes acting on starch, glycogen, and related oligo- and polysaccharides. These proteins catalyze the transformation of alpha-1,4 and alpha-1,6 glucosidic linkages with retention of the anomeric center. The protein is described as having 3 domains: A, B, C. A is a (beta/alpha) 8-barrel; B is a loop between the beta 3 strand and alpha 3 helix of A; C is the C-terminal extension characterized by a Greek key. The majority of the enzymes have an active site cleft found between domains A and B where a triad of catalytic residues (Asp, Glu and Asp) performs catalysis. Other members of this family have lost the catalytic activity as in the case of the human 4F2hc, or only have 2 residues that serve as the catalytic nucleophile and the acid/base, such as Thermus A4 beta-galactosidase with 2 Glu residues (GH42) and human alpha-galactosidase with 2 Asp residues (GH31). The family members are quite extensive and include: alpha amylase, maltosyltransferase, cyclodextrin glycotransferase, maltogenic amylase, neopullulanase, isoamylase, 1,4-alpha-D-glucan maltotetrahydrolase, 4-alpha-glucotransferase, oligo-1,6-glucosidase, amylosucrase, sucrose phosphorylase, and amylomaltase. | ||
PLN02361 | PLN02361 | 1.0e-169 | 474 | 865 | 398 | + alpha-amylase | ||
PLN02784 | PLN02784 | 0 | 62 | 867 | 836 | + alpha-amylase |
Gene Ontology | |
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GO Term | Description |
GO:0003824 | catalytic activity |
GO:0004556 | alpha-amylase activity |
GO:0005509 | calcium ion binding |
GO:0005975 | carbohydrate metabolic process |
GO:0043169 | cation binding |
Annotations - NR Download unfiltered results here | |||||||
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Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
GenBank | AAX33231.1 | 0 | 56 | 867 | 60 | 901 | plastid alpha-amylase [Malus x domestica] |
GenBank | EEC71386.1 | 0 | 1 | 868 | 1 | 876 | hypothetical protein OsI_03507 [Oryza sativa Indica Group] |
RefSeq | NP_001044062.1 | 0 | 1 | 868 | 1 | 876 | Os01g0715400 [Oryza sativa (japonica cultivar-group)] |
RefSeq | XP_002456247.1 | 0 | 88 | 868 | 26 | 820 | hypothetical protein SORBIDRAFT_03g032830 [Sorghum bicolor] |
RefSeq | XP_002520134.1 | 0 | 54 | 867 | 58 | 900 | alpha-amylase, putative [Ricinus communis] |
Annotations - PDB Download unfiltered results here | |||||||
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Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
PDB | 3bsg_A | 0 | 477 | 865 | 2 | 403 | A Chain A, Barley Alpha-Amylase Isozyme 1 (Amy1) H395a Mutant |
PDB | 1rpk_A | 0 | 477 | 865 | 2 | 403 | A Chain A, Barley Alpha-Amylase Isozyme 1 (Amy1) H395a Mutant |
PDB | 1p6w_A | 0 | 477 | 865 | 2 | 403 | A Chain A, Barley Alpha-Amylase Isozyme 1 (Amy1) H395a Mutant |
PDB | 1ht6_A | 0 | 477 | 865 | 2 | 403 | A Chain A, Crystal Structure At 1.5a Resolution Of The Barley Alpha- Amylase Isozyme 1 |
PDB | 2qps_A | 0 | 477 | 865 | 2 | 403 | A Chain A, "sugar Tongs" Mutant Y380a In Complex With Acarb |
Metabolic Pathways | |||
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Pathway Name | Reaction | EC | Protein Name |
starch degradation I | RXN-1823 | EC-3.2.1.1 | α-amylase |
starch degradation I | RXN-1825 | EC-3.2.1.1 | α-amylase |
EST Download unfiltered results here | ||||
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Hit | Length | Start | End | EValue |
EG631183 | 840 | 62 | 867 | 0 |
HO826981 | 404 | 465 | 867 | 0 |
DV475507 | 279 | 488 | 766 | 0 |
GO885960 | 285 | 563 | 847 | 0 |
DR932783 | 288 | 477 | 764 | 0 |
Sequence Alignments (This image is cropped. Click for full image.) |
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