| Basic Information | |
|---|---|
| Species | Panicum virgatum |
| Cazyme ID | Pavirv00034922m |
| Family | GT35 |
| Protein Properties | Length: 925 Molecular Weight: 104188 Isoelectric Point: 5.1445 |
| Chromosome | Chromosome/Scaffold: 032316 Start: 468 End: 6900 |
| Description | Glycosyl transferase, family 35 |
| View CDS | |
| External Links |
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| CAZyDB |
| Signature Domain Download full data set without filtering | |||
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| Family | Start | End | Evalue |
| GT35 | 119 | 465 | 0 |
| ALKQLGHNLEDVASQEPDAALGNGGLGRLASCFLDSLATLNYPAWGYGLRYKYGLFKQLITKNCQEEIAENWLEMGYPWEIVRNDLSYPVKFYGKVVEGT DGRRHWIGGENIKAVPHDVPIPGYKTRVTNNLRLWSTTVPAQDFDLGAFNAGDHTKAYEAHLNAEKICHVLYPGDESPEGKVLRLKQQYTLCSASLQDIV YRFEGRAGDSLNWEDFPSKVAVQMNDTHPTLCIPELMRILIDFKGLSWDEAWRITERTVAYTNHTVLPEALEKWSLDIMQKLLPRHVEIIEKIDEELINS IVSKYGTADTALLKNKLKEMRILDNVDLPASIAQLFVKPKAKNKSPT | |||
| GT35 | 529 | 918 | 0 |
| KLPRVVRMANLCVVGGHSVNGVAEIHSEIVKQDVFSSFYEMWPTKFQNKTNGVTPRRWIRFCNPELSTIISKWIGSDDWVLNTDKLAELKKFADNEDLHS EWRAAKLANKMKVVSLIRDKTGYIVSPDAMFDVQLLNILGIVYRYKKMKEMSAEERVKRFVPRVCIFGGKAFATYIQAKRIVKFITDVAATVNHDSDIGD LLKVIFVPDYNVSVAEALIPASELSQHISTAGMEASGTSNMKFAMNGCILIGTLDGANVEIREEVGEENFFLFGAKAHEIVGLRKERAEGKFVPDPRFQE VKEFVRCGVFGTYNYDELMGSLEGNEGYGRADYFLVGKDFPSYIECQEKVDEAYRDQKLWTKMSILNTAGSSKFSSDRTIHEYAKDIWDI | |||
| Full Sequence |
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| Protein Sequence Length: 925 Download |
| MRDGAGAEEA SRGRARQLLC PGIPSVLNSM DSSAIASNIK HYAEFKPFFA PEQISPLNAY 60 YATAKSVFDA LLINWNATYN YYDKMNVKQA YYLSMEFLQG RALTNAIGNL ELTGEYAEAL 120 KQLGHNLEDV ASQEPDAALG NGGLGRLASC FLDSLATLNY PAWGYGLRYK YGLFKQLITK 180 NCQEEIAENW LEMGYPWEIV RNDLSYPVKF YGKVVEGTDG RRHWIGGENI KAVPHDVPIP 240 GYKTRVTNNL RLWSTTVPAQ DFDLGAFNAG DHTKAYEAHL NAEKICHVLY PGDESPEGKV 300 LRLKQQYTLC SASLQDIVYR FEGRAGDSLN WEDFPSKVAV QMNDTHPTLC IPELMRILID 360 FKGLSWDEAW RITERTVAYT NHTVLPEALE KWSLDIMQKL LPRHVEIIEK IDEELINSIV 420 SKYGTADTAL LKNKLKEMRI LDNVDLPASI AQLFVKPKAK NKSPTKSKKK LLVKSLETIA 480 DVEEKTKLEE EEAEVLSETE EEKIESEEVE AGEEDSEDGL DPFVKSDPKL PRVVRMANLC 540 VVGGHSVNGV AEIHSEIVKQ DVFSSFYEMW PTKFQNKTNG VTPRRWIRFC NPELSTIISK 600 WIGSDDWVLN TDKLAELKKF ADNEDLHSEW RAAKLANKMK VVSLIRDKTG YIVSPDAMFD 660 VQLLNILGIV YRYKKMKEMS AEERVKRFVP RVCIFGGKAF ATYIQAKRIV KFITDVAATV 720 NHDSDIGDLL KVIFVPDYNV SVAEALIPAS ELSQHISTAG MEASGTSNMK FAMNGCILIG 780 TLDGANVEIR EEVGEENFFL FGAKAHEIVG LRKERAEGKF VPDPRFQEVK EFVRCGVFGT 840 YNYDELMGSL EGNEGYGRAD YFLVGKDFPS YIECQEKVDE AYRDQKLWTK MSILNTAGSS 900 KFSSDRTIHE YAKDIWDISP VTLP* |
| Functional Domains Download unfiltered results here | ||||||||
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| Cdd ID | Domain | E-Value | Start | End | Length | Domain Description | ||
| pfam00343 | Phosphorylase | 2.0e-135 | 119 | 442 | 325 | + Carbohydrate phosphorylase. The members of this family catalyze the formation of glucose 1-phosphate from one of the following polyglucoses; glycogen, starch, glucan or maltodextrin. | ||
| cd04300 | GT1_Glycogen_Phosphorylase | 0 | 531 | 918 | 407 | + This is a family of oligosaccharide phosphorylases. It includes yeast and mammalian glycogen phosphorylases, plant starch/glucan phosphorylase, as well as the maltodextrin phosphorylases of bacteria. The members of this family catalyze the breakdown of oligosaccharides into glucose-1-phosphate units. They are important allosteric enzymes in carbohydrate metabolism. The allosteric control mechanisms of yeast and mammalian members of this family are different from that of bacterial members. The members of this family belong to the GT-B structural superfamily of glycoslytransferases, which have characteristic N- and C-terminal domains each containing a typical Rossmann fold. The two domains have high structural homology despite minimal sequence homology. The large cleft that separates the two domains includes the catalytic center and permits a high degree of flexibility. | ||
| cd04300 | GT1_Glycogen_Phosphorylase | 0 | 36 | 443 | 412 | + This is a family of oligosaccharide phosphorylases. It includes yeast and mammalian glycogen phosphorylases, plant starch/glucan phosphorylase, as well as the maltodextrin phosphorylases of bacteria. The members of this family catalyze the breakdown of oligosaccharides into glucose-1-phosphate units. They are important allosteric enzymes in carbohydrate metabolism. The allosteric control mechanisms of yeast and mammalian members of this family are different from that of bacterial members. The members of this family belong to the GT-B structural superfamily of glycoslytransferases, which have characteristic N- and C-terminal domains each containing a typical Rossmann fold. The two domains have high structural homology despite minimal sequence homology. The large cleft that separates the two domains includes the catalytic center and permits a high degree of flexibility. | ||
| pfam00343 | Phosphorylase | 0 | 532 | 920 | 404 | + Carbohydrate phosphorylase. The members of this family catalyze the formation of glucose 1-phosphate from one of the following polyglucoses; glycogen, starch, glucan or maltodextrin. | ||
| TIGR02093 | P_ylase | 0 | 532 | 918 | 402 | + glycogen/starch/alpha-glucan phosphorylases. This family consists of phosphorylases. Members use phosphate to break alpha 1,4 linkages between pairs of glucose residues at the end of long glucose polymers, releasing alpha-D-glucose 1-phosphate. The nomenclature convention is to preface the name according to the natural substrate, as in glycogen phosphorylase, starch phosphorylase, maltodextrin phosphorylase, etc. Name differences among these substrates reflect differences in patterns of branching with alpha 1,6 linkages. Members include allosterically regulated and unregulated forms. A related family, TIGR02094, contains examples known to act well on particularly small alpha 1,4 glucans, as may be found after import from exogenous sources [Energy metabolism, Biosynthesis and degradation of polysaccharides]. | ||
| Gene Ontology | |
|---|---|
| GO Term | Description |
| GO:0004645 | phosphorylase activity |
| GO:0005975 | carbohydrate metabolic process |
| Annotations - NR Download unfiltered results here | |||||||
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| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| GenBank | AAK15695.1 | 0 | 23 | 924 | 19 | 928 | AF327055_1 alpha 1,4-glucan phosphorylase L isozyme [Oryza sativa] |
| GenBank | ACC59201.1 | 0 | 23 | 924 | 65 | 971 | plastid alpha-1,4-glucan phosphorylase [Triticum aestivum] |
| DDBJ | BAG49328.1 | 0 | 23 | 924 | 69 | 978 | plastidial starch phosphorylase 1 [Oryza sativa Japonica Group] |
| GenBank | EEC76210.1 | 0 | 23 | 924 | 69 | 964 | hypothetical protein OsI_13605 [Oryza sativa Indica Group] |
| GenBank | EEE59962.1 | 0 | 23 | 924 | 82 | 977 | hypothetical protein OsJ_12655 [Oryza sativa Japonica Group] |
| Annotations - PDB Download unfiltered results here | |||||||
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| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| PDB | 1ygp_B | 0 | 59 | 423 | 68 | 446 | A Chain A, Phosphorylated Form Of Yeast Glycogen Phosphorylase With Phosphate Bound In The Active Site. |
| PDB | 1ygp_B | 0 | 532 | 922 | 466 | 879 | A Chain A, Phosphorylated Form Of Yeast Glycogen Phosphorylase With Phosphate Bound In The Active Site. |
| PDB | 1ygp_A | 0 | 59 | 423 | 68 | 446 | A Chain A, Phosphorylated Form Of Yeast Glycogen Phosphorylase With Phosphate Bound In The Active Site. |
| PDB | 1ygp_A | 0 | 532 | 922 | 466 | 879 | A Chain A, Phosphorylated Form Of Yeast Glycogen Phosphorylase With Phosphate Bound In The Active Site. |
| PDB | 1qm5_B | 0 | 534 | 916 | 404 | 792 | A Chain A, Phosphorylase Recognition And Phosphorylysis Of Its Oligosaccharide Substrate: Answers To A Long Outstanding Question |
| Metabolic Pathways | |||
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| Pathway Name | Reaction | EC | Protein Name |
| starch degradation I | RXN-1826 | EC-2.4.1.1 | phosphorylase |
| Hydropathy |
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| EST Download unfiltered results here | ||||
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| Hit | Length | Start | End | EValue |
| HO797178 | 401 | 535 | 925 | 0 |
| HO778303 | 401 | 535 | 925 | 0 |
| HO778303 | 397 | 59 | 455 | 0 |
| HO613954 | 403 | 533 | 925 | 0 |
| HO613954 | 58 | 396 | 453 | 0.00000003 |
| Sequence Alignments (This image is cropped. Click for full image.) |
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