Basic Information | |
---|---|
Species | Panicum virgatum |
Cazyme ID | Pavirv00036935m |
Family | GT20 |
Protein Properties | Length: 851 Molecular Weight: 94906.7 Isoelectric Point: 6.7082 |
Chromosome | Chromosome/Scaffold: 000868 Start: 1 End: 7766 |
Description | trehalose-6-phosphate synthase |
View CDS |
External Links |
---|
CAZyDB |
Signature Domain Download full data set without filtering | |||
---|---|---|---|
Family | Start | End | Evalue |
GT20 | 124 | 592 | 0 |
MKQRLLVVANRLPVSANRRGEDQWSLEISAGGLVSALLGVKDVDAKWIGWAGVNVPDEVGQRALTRALAEKRCIPVFLDEEIVHQYYNGYCNNILWPLFH YLGLPQEDRLATTRNFESQFDAYKRANQMFADVVYQHYQEGDVIWCHDYHLMFLPKCLKDHDINMKVGWFLHTPFPSSEIYRTLPSRLELLRSVLCADLV GFHTYDYARHFVSACTRILGLEGTPEGVEDQGRLTRVAAFPIGIDSDRFKRALELPAVKRHINELTHRFAGRKVMLGVDRLDMIKGIPQKILAFEKFLEE NPDWNDKVVLLQIAVPTRTDVPEYQKLTSQVHEIVGRINGRFGTLSAVPIHHLDRSLDFHALCALYAVTDVALVTSLRDGMNLVSYEYVACQGSKKGVLI LSEFAGAAQSLGAGAILVNPWNITEVADSIRHALTMSSDEREKRHRHNYAHVTTHTAQDWAETFVCELN |
Full Sequence |
---|
Protein Sequence Length: 851 Download |
MSSDAAGGQR SSNRARGDAA LMPTSSPFTG DGGGAGSPTR VERMLREREH SRRHLFASSD 60 AMDTDGALPA FASAVAFVAD GVQSPGRASP ANMEDAGGAA PGHAARPPLA GSRSGFRRLG 120 LRGMKQRLLV VANRLPVSAN RRGEDQWSLE ISAGGLVSAL LGVKDVDAKW IGWAGVNVPD 180 EVGQRALTRA LAEKRCIPVF LDEEIVHQYY NGYCNNILWP LFHYLGLPQE DRLATTRNFE 240 SQFDAYKRAN QMFADVVYQH YQEGDVIWCH DYHLMFLPKC LKDHDINMKV GWFLHTPFPS 300 SEIYRTLPSR LELLRSVLCA DLVGFHTYDY ARHFVSACTR ILGLEGTPEG VEDQGRLTRV 360 AAFPIGIDSD RFKRALELPA VKRHINELTH RFAGRKVMLG VDRLDMIKGI PQKILAFEKF 420 LEENPDWNDK VVLLQIAVPT RTDVPEYQKL TSQVHEIVGR INGRFGTLSA VPIHHLDRSL 480 DFHALCALYA VTDVALVTSL RDGMNLVSYE YVACQGSKKG VLILSEFAGA AQSLGAGAIL 540 VNPWNITEVA DSIRHALTMS SDEREKRHRH NYAHVTTHTA QDWAETFVCE LNDTVAEAQL 600 RTRQVPPSLP SQTAIQQYLR SKNRLLILGF NSTLTEPVES SGRRGGDQIK EMELKLHPDL 660 KGPLKALCED EHTTVIVLSG SDRSVLDENF GEFKMWLAAE HGMFLRPTYG EWMTTMPEHL 720 NMDWVDSVKH VFEYFTERTP RSHFEHRETS FVWNYKYADV EFGRLQARDM LQHLWTGPIS 780 NAAVDVVQGS HSVEVRSVGV TKGAAIDRIL GEIVHSENMD TPIDYVLCVG HFLGKEEKNT 840 EPISCSRVSV * |
Functional Domains Download unfiltered results here | ||||||
---|---|---|---|---|---|---|
Cdd ID | Domain | E-Value | Start | End | Length | Domain Description |
PLN03064 | PLN03064 | 0 | 31 | 837 | 808 | + alpha,alpha-trehalose-phosphate synthase (UDP-forming); Provisional |
PLN03063 | PLN03063 | 0 | 125 | 837 | 713 | + alpha,alpha-trehalose-phosphate synthase (UDP-forming); Provisional |
PRK14501 | PRK14501 | 0 | 127 | 810 | 689 | + putative bifunctional trehalose-6-phosphate synthase/HAD hydrolase subfamily IIB; Provisional |
pfam00982 | Glyco_transf_20 | 0 | 126 | 593 | 474 | + Glycosyltransferase family 20. Members of this family belong to glycosyl transferase family 20. OtsA (Trehalose-6-phosphate synthase) is homologous to regions in the subunits of yeast trehalose-6-phosphate synthase/phosphate complex. |
TIGR02400 | trehalose_OtsA | 0 | 127 | 593 | 469 | + alpha,alpha-trehalose-phosphate synthase [UDP-forming]. This enzyme catalyzes the key, penultimate step in biosynthesis of trehalose, a compatible solute made as an osmoprotectant in some species in all three domains of life. The gene symbol OtsA stands for osmotically regulated trehalose synthesis A. Trehalose helps protect against both osmotic and thermal stresses, and is made from two glucose subunits. This model excludes glucosylglycerol-phosphate synthase, an enzyme of an analogous osmoprotectant system in many cyanobacterial strains. This model does not identify archaeal examples, as they are more divergent than glucosylglycerol-phosphate synthase. Sequences that score in the gray zone between the trusted and noise cutoffs include a number of yeast multidomain proteins in which the N-terminal domain may be functionally equivalent to this family. The gray zone also includes the OtsA of Cornyebacterium glutamicum (and related species), shown to be responsible for synthesis of only trace amounts of trehalose while the majority is synthesized by the TreYZ pathway; the significance of OtsA in this species is unclear (see Wolf, et al., PMID:12890033) [Cellular processes, Adaptations to atypical conditions]. |
Gene Ontology | |
---|---|
GO Term | Description |
GO:0003824 | catalytic activity |
GO:0005992 | trehalose biosynthetic process |
Annotations - NR Download unfiltered results here | |||||||
---|---|---|---|---|---|---|---|
Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
GenBank | ACI16353.1 | 0 | 124 | 837 | 1 | 714 | trehalose-6-P synthase [Triticum aestivum] |
GenBank | EAY98715.1 | 0 | 124 | 837 | 1 | 714 | hypothetical protein OsI_20647 [Oryza sativa Indica Group] |
RefSeq | NP_001123593.2 | 0 | 22 | 837 | 1 | 804 | trehalose-6-phosphate synthase [Zea mays] |
RefSeq | XP_002331300.1 | 0 | 115 | 837 | 76 | 797 | predicted protein [Populus trichocarpa] |
RefSeq | XP_002440086.1 | 0 | 1 | 837 | 1 | 839 | hypothetical protein SORBIDRAFT_09g025790 [Sorghum bicolor] |
Annotations - PDB Download unfiltered results here | |||||||
---|---|---|---|---|---|---|---|
Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
PDB | 2wtx_D | 0 | 127 | 603 | 3 | 466 | A Chain A, Insight Into The Mechanism Of Enzymatic Glycosyltransfer With Retention Through The Synthesis And Analysis Of Bisubstrate Glycomimetics Of Trehalose-6-Phosphate Synthase |
PDB | 2wtx_C | 0 | 127 | 603 | 3 | 466 | A Chain A, Insight Into The Mechanism Of Enzymatic Glycosyltransfer With Retention Through The Synthesis And Analysis Of Bisubstrate Glycomimetics Of Trehalose-6-Phosphate Synthase |
PDB | 2wtx_B | 0 | 127 | 603 | 3 | 466 | A Chain A, Insight Into The Mechanism Of Enzymatic Glycosyltransfer With Retention Through The Synthesis And Analysis Of Bisubstrate Glycomimetics Of Trehalose-6-Phosphate Synthase |
PDB | 2wtx_A | 0 | 127 | 603 | 3 | 466 | A Chain A, Insight Into The Mechanism Of Enzymatic Glycosyltransfer With Retention Through The Synthesis And Analysis Of Bisubstrate Glycomimetics Of Trehalose-6-Phosphate Synthase |
PDB | 1uqu_B | 0 | 127 | 603 | 3 | 466 | A Chain A, Trehalose-6-Phosphate From E. Coli Bound With Udp-Glucose. |
Metabolic Pathways | |||
---|---|---|---|
Pathway Name | Reaction | EC | Protein Name |
trehalose biosynthesis I | TREHALOSE6PSYN-RXN | EC-2.4.1.15 | α,α-trehalose-phosphate synthase (UDP-forming) |
EST Download unfiltered results here | ||||
---|---|---|---|---|
Hit | Length | Start | End | EValue |
CB651133 | 281 | 153 | 433 | 0 |
EH189441 | 295 | 495 | 789 | 0 |
DW069910 | 288 | 213 | 500 | 0 |
CB629539 | 271 | 305 | 575 | 0 |
EH189441 | 57 | 767 | 823 | 0.018 |
Sequence Alignments (This image is cropped. Click for full image.) |
---|