| Basic Information | |
|---|---|
| Species | Panicum virgatum |
| Cazyme ID | Pavirv00040343m |
| Family | GH32 |
| Protein Properties | Length: 478 Molecular Weight: 51685.4 Isoelectric Point: 5.0699 |
| Chromosome | Chromosome/Scaffold: 058110 Start: 7 End: 3588 |
| Description | Glycosyl hydrolases family 32 protein |
| View CDS | |
| External Links |
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| CAZyDB |
| Signature Domain Download full data set without filtering | |||
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| Family | Start | End | Evalue |
| GH32 | 2 | 268 | 0 |
| VPDHWYDANGVWSGSATRLPDGRIVMLYTGSTTESVQVQNLAEPADPSDPLLRRWVKSAANPILVPPPGIGLKDFRDPTTAWRVPNDTAWRVAIGSKDRS HAGLALVYRTADFVRYDPAPALMRVVPGTGMWECVDFYPVAAVAGGVENGLETSVPPGPGVKHVVKASLDDDKRDYYAIGTYDAGADAWTPEDAANDVGI GLLYDYGKFYASKTFYDPVLRRRVLWGWVGETDSERAAILKGWASVQSIPRTVLLDTKTGSNLLQWP | |||
| Full Sequence |
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| Protein Sequence Length: 478 Download |
| MVPDHWYDAN GVWSGSATRL PDGRIVMLYT GSTTESVQVQ NLAEPADPSD PLLRRWVKSA 60 ANPILVPPPG IGLKDFRDPT TAWRVPNDTA WRVAIGSKDR SHAGLALVYR TADFVRYDPA 120 PALMRVVPGT GMWECVDFYP VAAVAGGVEN GLETSVPPGP GVKHVVKASL DDDKRDYYAI 180 GTYDAGADAW TPEDAANDVG IGLLYDYGKF YASKTFYDPV LRRRVLWGWV GETDSERAAI 240 LKGWASVQSI PRTVLLDTKT GSNLLQWPVV EVENLRMSGK SFDGVALDRG SIVPLDVGKA 300 TQLDIEAVFE VDAAAVEGVT EADVGFNCST SAGAAGRGLL GPFGLLVLAD DDLSEQTAAY 360 FYLAKGTDGS LKTFFCQDEL RASRANDLVK RVYGSSVPVL HGENLSVRIL VDHSIVESFA 420 QGGRTCVTSR VYPTRAIYDS ARVFLFNNAT SVRVTAKSVK VWQMNSAYIR PYPGTSL* 480 |
| Functional Domains Download unfiltered results here | ||||||||
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| Cdd ID | Domain | E-Value | Start | End | Length | Domain Description | ||
| TIGR01322 | scrB_fam | 2.0e-23 | 3 | 433 | 453 | + sucrose-6-phosphate hydrolase. [Energy metabolism, Biosynthesis and degradation of polysaccharides]. | ||
| COG1621 | SacC | 9.0e-31 | 3 | 435 | 452 | + Beta-fructosidases (levanase/invertase) [Carbohydrate transport and metabolism] | ||
| cd08996 | GH32_B_Fructosidase | 2.0e-49 | 1 | 271 | 276 | + Glycosyl hydrolase family 32, beta-fructosidases. Glycosyl hydrolase family GH32 cleaves sucrose into fructose and glucose via beta-fructofuranosidase activity, producing invert sugar that is a mixture of dextrorotatory D-glucose and levorotatory D-fructose, thus named invertase (EC 3.2.1.26). This family also contains other fructofuranosidases such as inulinase (EC 3.2.1.7), exo-inulinase (EC 3.2.1.80), levanase (EC 3.2.1.65), and transfructosidases such sucrose:sucrose 1-fructosyltransferase (EC 2.4.1.99), fructan:fructan 1-fructosyltransferase (EC 2.4.1.100), sucrose:fructan 6-fructosyltransferase (EC 2.4.1.10), fructan:fructan 6G-fructosyltransferase (EC 2.4.1.243) and levan fructosyltransferases (EC 2.4.1.-). These retaining enzymes (i.e. they retain the configuration at anomeric carbon atom of the substrate) catalyze hydrolysis in two steps involving a covalent glycosyl enzyme intermediate: an aspartate located close to the N-terminus acts as the catalytic nucleophile and a glutamate acts as the general acid/base; a conserved aspartate residue in the Arg-Asp-Pro (RDP) motif stabilizes the transition state. These enzymes are predicted to display a 5-fold beta-propeller fold as found for GH43 and CH68. The breakdown of sucrose is widely used as a carbon or energy source by bacteria, fungi, and plants. Invertase is used commercially in the confectionery industry, since fructose has a sweeter taste than sucrose and a lower tendency to crystallize. A common structural feature of all these enzymes is a 5-bladed beta-propeller domain, similar to GH43, that contains the catalytic acid and catalytic base. A long V-shaped groove, partially enclosed at one end, forms a single extended substrate-binding surface across the face of the propeller. | ||
| pfam00251 | Glyco_hydro_32N | 1.0e-92 | 1 | 268 | 275 | + Glycosyl hydrolases family 32 N-terminal domain. This domain corresponds to the N-terminal domain of glycosyl hydrolase family 32 which forms a five bladed beta propeller structure. | ||
| smart00640 | Glyco_32 | 1.0e-127 | 1 | 423 | 432 | + Glycosyl hydrolases family 32. | ||
| Annotations - NR Download unfiltered results here | |||||||
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| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| GenBank | ACN34201.1 | 0 | 1 | 476 | 1 | 482 | unknown [Zea mays] |
| EMBL | CAH67112.1 | 0 | 1 | 472 | 175 | 647 | H0502G05.3 [Oryza sativa (indica cultivar-group)] |
| RefSeq | NP_001053422.1 | 0 | 1 | 472 | 182 | 654 | Os04g0535600 [Oryza sativa (japonica cultivar-group)] |
| Swiss-Prot | P49175 | 0 | 1 | 476 | 186 | 667 | INV1_MAIZE RecName: Full=Beta-fructofuranosidase 1; AltName: Full=Sucrose 1; AltName: Full=Invertase 1; Flags: Precursor |
| RefSeq | XP_002446857.1 | 0 | 1 | 477 | 199 | 679 | hypothetical protein SORBIDRAFT_06g023760 [Sorghum bicolor] |
| Annotations - PDB Download unfiltered results here | |||||||
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| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| PDB | 3ugh_B | 0 | 1 | 472 | 80 | 544 | A Chain A, Plant Carboxylesterase Aecxe1 From Actinidia Eriantha With Acyl Adduct |
| PDB | 3ugh_A | 0 | 1 | 472 | 80 | 544 | A Chain A, Plant Carboxylesterase Aecxe1 From Actinidia Eriantha With Acyl Adduct |
| PDB | 3ugg_B | 0 | 1 | 472 | 80 | 544 | A Chain A, Plant Carboxylesterase Aecxe1 From Actinidia Eriantha With Acyl Adduct |
| PDB | 3ugg_A | 0 | 1 | 472 | 80 | 544 | A Chain A, Plant Carboxylesterase Aecxe1 From Actinidia Eriantha With Acyl Adduct |
| PDB | 3ugf_B | 0 | 1 | 472 | 80 | 544 | A Chain A, Crystal Structure Of A 6-Sst6-Sft From Pachysandra Terminalis |
| Metabolic Pathways | |||
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| Pathway Name | Reaction | EC | Protein Name |
| sucrose degradation III | RXN-1461 | EC-3.2.1.26 | β-fructofuranosidase |
| Hydropathy |
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| EST Download unfiltered results here | ||||
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| Hit | Length | Start | End | EValue |
| FE636262 | 235 | 71 | 305 | 0 |
| FL909048 | 237 | 238 | 474 | 0 |
| GW667497 | 307 | 168 | 474 | 0 |
| GO882338 | 345 | 91 | 429 | 0 |
| CB651234 | 259 | 216 | 472 | 0 |
| Sequence Alignments (This image is cropped. Click for full image.) |
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