| Basic Information | |
|---|---|
| Species | Panicum virgatum |
| Cazyme ID | Pavirv00047450m |
| Family | GH32 |
| Protein Properties | Length: 594 Molecular Weight: 64784.5 Isoelectric Point: 5.0856 |
| Chromosome | Chromosome/Scaffold: 0162796 Start: 317 End: 2534 |
| Description | beta-fructofuranosidase 5 |
| View CDS | |
| External Links |
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| CAZyDB |
| Signature Domain Download full data set without filtering | |||
|---|---|---|---|
| Family | Start | End | Evalue |
| GH32 | 41 | 363 | 0 |
| HFQPAKNWQNGPVYYNGMYHLFYQYNPHGALWDVGNLSWGHSVSGDLVNWAALGNALDPTAPFDANGCASGSVTILPDGQPVILYSGIDAERRQVQNIAL PKNPRDPLLREWTKPSYNPVVPLPADVSADNFRDPTTAWLGRDGLWRFAISAVATGVGTTLVYRSADFLRWERNAAPLHASRDAVMAECPDLFPVAARGA EGLDTSARGPGVRHVLKVSMPDTLQDYYTVGRYDDEADTFTPDEDVARGGGDDDYRSWRRIDHGHLYASKTFFDARKNRRVLWAWVNESDSEADDVARGW SGLQSFPRALWLDPAGRQLLQWP | |||
| Full Sequence |
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| Protein Sequence Length: 594 Download |
| MAALRLAVSN VILCLLLPSS SSSSSPICAA RMQDHVRTAY HFQPAKNWQN GPVYYNGMYH 60 LFYQYNPHGA LWDVGNLSWG HSVSGDLVNW AALGNALDPT APFDANGCAS GSVTILPDGQ 120 PVILYSGIDA ERRQVQNIAL PKNPRDPLLR EWTKPSYNPV VPLPADVSAD NFRDPTTAWL 180 GRDGLWRFAI SAVATGVGTT LVYRSADFLR WERNAAPLHA SRDAVMAECP DLFPVAARGA 240 EGLDTSARGP GVRHVLKVSM PDTLQDYYTV GRYDDEADTF TPDEDVARGG GDDDYRSWRR 300 IDHGHLYASK TFFDARKNRR VLWAWVNESD SEADDVARGW SGLQSFPRAL WLDPAGRQLL 360 QWPVEEIETL RRGRAALLGA EVESGGLREI GGIAGSQADV DVVFEIPSLE RAEGLDGNRL 420 LDADALCGEK GASVQGGVGP FGLLVMASGD DLREHTAVFF RVFRLLHGEH TVLMCTDLTR 480 SSTKAGVYRP THGGFVNVDI EKDKSISLRT LIDHSIIESF GGGGRTCMTA RVYPEHAATG 540 SSHLYVFNNG SDAVKVPRLE AWELATASVN VVPEEEEDGL AASSNMCTSW AYY* 600 |
| Functional Domains Download unfiltered results here | ||||||
|---|---|---|---|---|---|---|
| Cdd ID | Domain | E-Value | Start | End | Length | Domain Description |
| TIGR01322 | scrB_fam | 3.0e-43 | 31 | 534 | 526 | + sucrose-6-phosphate hydrolase. [Energy metabolism, Biosynthesis and degradation of polysaccharides]. |
| COG1621 | SacC | 1.0e-57 | 33 | 542 | 528 | + Beta-fructosidases (levanase/invertase) [Carbohydrate transport and metabolism] |
| cd08996 | GH32_B_Fructosidase | 1.0e-82 | 50 | 366 | 323 | + Glycosyl hydrolase family 32, beta-fructosidases. Glycosyl hydrolase family GH32 cleaves sucrose into fructose and glucose via beta-fructofuranosidase activity, producing invert sugar that is a mixture of dextrorotatory D-glucose and levorotatory D-fructose, thus named invertase (EC 3.2.1.26). This family also contains other fructofuranosidases such as inulinase (EC 3.2.1.7), exo-inulinase (EC 3.2.1.80), levanase (EC 3.2.1.65), and transfructosidases such sucrose:sucrose 1-fructosyltransferase (EC 2.4.1.99), fructan:fructan 1-fructosyltransferase (EC 2.4.1.100), sucrose:fructan 6-fructosyltransferase (EC 2.4.1.10), fructan:fructan 6G-fructosyltransferase (EC 2.4.1.243) and levan fructosyltransferases (EC 2.4.1.-). These retaining enzymes (i.e. they retain the configuration at anomeric carbon atom of the substrate) catalyze hydrolysis in two steps involving a covalent glycosyl enzyme intermediate: an aspartate located close to the N-terminus acts as the catalytic nucleophile and a glutamate acts as the general acid/base; a conserved aspartate residue in the Arg-Asp-Pro (RDP) motif stabilizes the transition state. These enzymes are predicted to display a 5-fold beta-propeller fold as found for GH43 and CH68. The breakdown of sucrose is widely used as a carbon or energy source by bacteria, fungi, and plants. Invertase is used commercially in the confectionery industry, since fructose has a sweeter taste than sucrose and a lower tendency to crystallize. A common structural feature of all these enzymes is a 5-bladed beta-propeller domain, similar to GH43, that contains the catalytic acid and catalytic base. A long V-shaped groove, partially enclosed at one end, forms a single extended substrate-binding surface across the face of the propeller. |
| pfam00251 | Glyco_hydro_32N | 6.0e-130 | 41 | 363 | 334 | + Glycosyl hydrolases family 32 N-terminal domain. This domain corresponds to the N-terminal domain of glycosyl hydrolase family 32 which forms a five bladed beta propeller structure. |
| smart00640 | Glyco_32 | 9.0e-154 | 41 | 524 | 497 | + Glycosyl hydrolases family 32. |
| Annotations - NR Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| EMBL | CAE03580.1 | 0 | 1 | 570 | 3 | 574 | OSJNBa0087O24.3 [Oryza sativa (japonica cultivar-group)] |
| EMBL | CAH65975.1 | 0 | 1 | 570 | 3 | 574 | H1005F08.4 [Oryza sativa (indica cultivar-group)] |
| RefSeq | NP_001054172.1 | 0 | 1 | 570 | 3 | 574 | Os04g0664800 [Oryza sativa (japonica cultivar-group)] |
| Swiss-Prot | Q56UD0 | 0 | 1 | 570 | 3 | 577 | INV6_ORYSJ RecName: Full=Beta-fructofuranosidase, insoluble isoenzyme 6; AltName: Full=Sucrose hydrolase 6; AltName: Full=Invertase 6; AltName: Full=Cell wall beta-fructosidase 6; AltName: Full=OsCIN6; Flags: Precursor |
| RefSeq | XP_002448711.1 | 0 | 27 | 592 | 29 | 587 | hypothetical protein SORBIDRAFT_06g031910 [Sorghum bicolor] |
| Annotations - PDB Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| PDB | 2aey_A | 0 | 31 | 571 | 2 | 535 | A Chain A, Crystal Structure Of Human Glcat-S Apo Form |
| PDB | 2ade_A | 0 | 31 | 571 | 2 | 535 | A Chain A, Crystal Structure Of Human Glcat-S Apo Form |
| PDB | 2add_A | 0 | 31 | 571 | 2 | 535 | A Chain A, Crystal Structure Of Human Glcat-S Apo Form |
| PDB | 1st8_A | 0 | 31 | 571 | 2 | 535 | A Chain A, Crystal Structure Of Fructan 1-Exohydrolase Iia From Cichorium Intybus |
| PDB | 2aez_A | 0 | 31 | 571 | 2 | 535 | A Chain A, Crystal Structure Of Fructan 1-Exohydrolase Iia (E201q) From Cichorium Intybus In Complex With 1-Kestose |
| Metabolic Pathways | |||
|---|---|---|---|
| Pathway Name | Reaction | EC | Protein Name |
| sucrose degradation III | RXN-1461 | EC-3.2.1.26 | β-fructofuranosidase |
