Basic Information | |
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Species | Panicum virgatum |
Cazyme ID | Pavirv00050471m |
Family | GH16 |
Protein Properties | Length: 224 Molecular Weight: 24358.3 Isoelectric Point: 6.0769 |
Chromosome | Chromosome/Scaffold: 074057 Start: 2191 End: 3681 |
Description | xyloglucan endotransglucosylase/hydrolase 28 |
View CDS |
External Links |
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CAZyDB |
Signature Domain Download full data set without filtering | |||
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Family | Start | End | Evalue |
GH16 | 73 | 216 | 2.6e-25 |
STGAGFASQDLFLHGFFSAAIKLPADYAAGVVVAFYLSNGDVYEKTHDELDFEFLGNVRGREWRVQTNVYGNGSTHACREERYDLPFDPTDDFHHYSILW TSQRIIFYVDETPIREVVRTAAMGAAFPSKPMSLYATIWDGSTW |
Full Sequence |
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Protein Sequence Length: 224 Download |
MASGASSSPP LSVLSLAAVV VLLLAARGGA RPPHSLRGVR PLAFDEGYAQ IFGSSNLALL 60 RDGRRVHLAL DESTGAGFAS QDLFLHGFFS AAIKLPADYA AGVVVAFYLS NGDVYEKTHD 120 ELDFEFLGNV RGREWRVQTN VYGNGSTHAC REERYDLPFD PTDDFHHYSI LWTSQRIIFY 180 VDETPIREVV RTAAMGAAFP SKPMSLYATI WDGSTWANAS ASH* |
Functional Domains Download unfiltered results here | ||||||
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Cdd ID | Domain | E-Value | Start | End | Length | Domain Description |
cd02175 | GH16_lichenase | 2.0e-22 | 74 | 219 | 150 | + lichenase, member of glycosyl hydrolase family 16. Lichenase, also known as 1,3-1,4-beta-glucanase, is a member of glycosyl hydrolase family 16, that specifically cleaves 1,4-beta-D-glucosidic bonds in mixed-linked beta glucans that also contain 1,3-beta-D-glucosidic linkages. Natural substrates of beta-glucanase are beta-glucans from grain endosperm cell walls or lichenan from the Islandic moss, Cetraria islandica. This protein is found not only in bacteria but also in anaerobic fungi. This domain includes two seven-stranded antiparallel beta-sheets that are adjacent to one another forming a compact, jellyroll beta-sandwich structure. |
cd00413 | Glyco_hydrolase_16 | 1.0e-22 | 74 | 222 | 153 | + glycosyl hydrolase family 16. The O-Glycosyl hydrolases are a widespread group of enzymes that hydrolyse the glycosidic bond between two or more carbohydrates, or between a carbohydrate and a non-carbohydrate moiety. A glycosyl hydrolase classification system based on sequence similarity has led to the definition of more than 95 different families inlcuding glycosyl hydrolase family 16. Family 16 includes lichenase, xyloglucan endotransglycosylase (XET), beta-agarase, kappa-carrageenase, endo-beta-1,3-glucanase, endo-beta-1,3-1,4-glucanase, and endo-beta-galactosidase, all of which have a conserved jelly roll fold with a deep active site channel harboring the catalytic residues. |
PLN03161 | PLN03161 | 2.0e-38 | 52 | 217 | 170 | + Probable xyloglucan endotransglucosylase/hydrolase protein; Provisional |
pfam00722 | Glyco_hydro_16 | 2.0e-63 | 44 | 219 | 176 | + Glycosyl hydrolases family 16. |
cd02176 | GH16_XET | 3.0e-91 | 42 | 217 | 177 | + Xyloglucan endotransglycosylase, member of glycosyl hydrolase family 16. Xyloglucan endotransglycosylases (XETs) cleave and religate xyloglucan polymers in plant cell walls via a transglycosylation mechanism. Xyloglucan is a soluble hemicellulose with a backbone of beta-1,4-linked glucose units, partially substituted with alpha-1,6-linked xylopyranose branches. It binds noncovalently to cellulose, cross-linking the adjacent cellulose microfibrils, giving it a key structural role as a matrix polymer. Therefore, XET plays an important role in all plant processes that require cell wall remodeling. |
Gene Ontology | |
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GO Term | Description |
GO:0004553 | hydrolase activity, hydrolyzing O-glycosyl compounds |
GO:0005975 | carbohydrate metabolic process |
Annotations - NR Download unfiltered results here | |||||||
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Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
GenBank | EAZ09009.1 | 0 | 28 | 218 | 26 | 219 | hypothetical protein OsI_31270 [Oryza sativa Indica Group] |
GenBank | EAZ44637.1 | 0 | 28 | 218 | 26 | 219 | hypothetical protein OsJ_29257 [Oryza sativa Japonica Group] |
RefSeq | XP_002315842.1 | 0 | 42 | 223 | 34 | 215 | predicted protein [Populus trichocarpa] |
RefSeq | XP_002462345.1 | 0 | 30 | 218 | 30 | 218 | hypothetical protein SORBIDRAFT_02g024120 [Sorghum bicolor] |
RefSeq | XP_002465580.1 | 0 | 42 | 219 | 39 | 216 | hypothetical protein SORBIDRAFT_01g041510 [Sorghum bicolor] |
Annotations - PDB Download unfiltered results here | |||||||
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Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
PDB | 2vh9_B | 1.4013e-45 | 9 | 223 | 2 | 216 | A Chain A, Crystal Structure Of Nxg1-Deltayniig In Complex With Xllg, A Xyloglucan Derived Oligosaccharide |
PDB | 2vh9_A | 1.4013e-45 | 9 | 223 | 2 | 216 | A Chain A, Crystal Structure Of Nxg1-Deltayniig In Complex With Xllg, A Xyloglucan Derived Oligosaccharide |
PDB | 2uwb_B | 2.8026e-45 | 39 | 223 | 15 | 193 | A Chain A, Crystal Structure Of The Nasturtium Seedling Mutant Xyloglucanase Isoform Nxg1-Delta-Yniig |
PDB | 2uwb_A | 2.8026e-45 | 39 | 223 | 15 | 193 | A Chain A, Crystal Structure Of The Nasturtium Seedling Mutant Xyloglucanase Isoform Nxg1-Delta-Yniig |
PDB | 2uwc_B | 5.60519e-45 | 39 | 223 | 14 | 197 | A Chain A, Crystal Structure Of Nasturtium Xyloglucan Hydrolase Isoform Nxg2 |