y
Basic Information | |
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Species | Panicum virgatum |
Cazyme ID | Pavirv00053436m |
Family | GH13 |
Protein Properties | Length: 435 Molecular Weight: 47902.3 Isoelectric Point: 6.6216 |
Chromosome | Chromosome/Scaffold: 0113813 Start: 1688 End: 3444 |
Description | alpha-amylase-like |
View CDS |
External Links |
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CAZyDB |
Signature Domain Download full data set without filtering | |||
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Family | Start | End | Evalue |
GH13 | 38 | 343 | 1.96182e-44 |
KQGGWYNSLKAQVDDIAKAGVTHVWLPPPSHSVAPQGYLPGRLYDLDASRYGTAAELKSLIAAFHGRGIQCVADVVINHRCAEKKDARGVYCIFEGGTPD DRLDWGPGMICSDDTQYSDGTGHRDTGEGFAAAPDIDHLNPRVQRELTGWLNWLKSDVGFDGWRLDFAKGYSPAIAKMYVETTRPSFVVAEIWNSLSYTD GKPSPNQDQCRQELVDWVDAVGAPAMAFDFTTKGLLQAGVQGELWRLRDGSGKAAGLIGWTPEKAVTFVDNHDTGSTQKLWPFPSDKVMQGYAYILTHPG VPCIFY |
Full Sequence |
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Protein Sequence Length: 435 Download |
MKHSSKFCLL FSLVVVALCS LVQAQVLFQG FNWESYKKQG GWYNSLKAQV DDIAKAGVTH 60 VWLPPPSHSV APQGYLPGRL YDLDASRYGT AAELKSLIAA FHGRGIQCVA DVVINHRCAE 120 KKDARGVYCI FEGGTPDDRL DWGPGMICSD DTQYSDGTGH RDTGEGFAAA PDIDHLNPRV 180 QRELTGWLNW LKSDVGFDGW RLDFAKGYSP AIAKMYVETT RPSFVVAEIW NSLSYTDGKP 240 SPNQDQCRQE LVDWVDAVGA PAMAFDFTTK GLLQAGVQGE LWRLRDGSGK AAGLIGWTPE 300 KAVTFVDNHD TGSTQKLWPF PSDKVMQGYA YILTHPGVPC IFYDHMFDWN LKQEISTLAA 360 IRARNGIHAG SKLRILLADA DAYVAVVDEV VMVKIGTRYD VSSVIPSDFH PAAHGKDYCV 420 WEKGSLRVPA GRHL* 480 |
Functional Domains Download unfiltered results here | ||||||||
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Cdd ID | Domain | E-Value | Start | End | Length | Domain Description | ||
PRK09441 | PRK09441 | 3.0e-51 | 23 | 362 | 425 | + cytoplasmic alpha-amylase; Reviewed | ||
PLN02784 | PLN02784 | 6.0e-133 | 25 | 423 | 400 | + alpha-amylase | ||
PLN02361 | PLN02361 | 3.0e-136 | 25 | 423 | 405 | + alpha-amylase | ||
cd11314 | AmyAc_arch_bac_plant_AmyA | 9.0e-166 | 26 | 373 | 351 | + Alpha amylase catalytic domain found in archaeal, bacterial, and plant Alpha-amylases (also called 1,4-alpha-D-glucan-4-glucanohydrolase). AmyA (EC 3.2.1.1) catalyzes the hydrolysis of alpha-(1,4) glycosidic linkages of glycogen, starch, related polysaccharides, and some oligosaccharides. This group includes AmyA from bacteria, archaea, water fleas, and plants. The Alpha-amylase family comprises the largest family of glycoside hydrolases (GH), with the majority of enzymes acting on starch, glycogen, and related oligo- and polysaccharides. These proteins catalyze the transformation of alpha-1,4 and alpha-1,6 glucosidic linkages with retention of the anomeric center. The protein is described as having 3 domains: A, B, C. A is a (beta/alpha) 8-barrel; B is a loop between the beta 3 strand and alpha 3 helix of A; C is the C-terminal extension characterized by a Greek key. The majority of the enzymes have an active site cleft found between domains A and B where a triad of catalytic residues (Asp, Glu and Asp) performs catalysis. Other members of this family have lost the catalytic activity as in the case of the human 4F2hc, or only have 2 residues that serve as the catalytic nucleophile and the acid/base, such as Thermus A4 beta-galactosidase with 2 Glu residues (GH42) and human alpha-galactosidase with 2 Asp residues (GH31). The family members are quite extensive and include: alpha amylase, maltosyltransferase, cyclodextrin glycotransferase, maltogenic amylase, neopullulanase, isoamylase, 1,4-alpha-D-glucan maltotetrahydrolase, 4-alpha-glucotransferase, oligo-1,6-glucosidase, amylosucrase, sucrose phosphorylase, and amylomaltase. | ||
PLN00196 | PLN00196 | 0 | 4 | 423 | 425 | + alpha-amylase; Provisional |
Gene Ontology | |
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GO Term | Description |
GO:0003824 | catalytic activity |
GO:0004556 | alpha-amylase activity |
GO:0005509 | calcium ion binding |
GO:0005975 | carbohydrate metabolic process |
GO:0043169 | cation binding |
Annotations - NR Download unfiltered results here | |||||||
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Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
PIR | 0 | 19 | 434 | 20 | 435 | UP10_LACSN Unknown protein 10 from 2D-PAGE | |
GenBank | AAA33895.1 | 0 | 19 | 434 | 20 | 435 | alpha-amylase [Oryza sativa Japonica Group] |
GenBank | ACN34260.1 | 0 | 21 | 434 | 25 | 441 | unknown [Zea mays] |
RefSeq | NP_001062024.1 | 0 | 19 | 434 | 60 | 476 | Os08g0473900 [Oryza sativa (japonica cultivar-group)] |
Swiss-Prot | P27933 | 0 | 19 | 434 | 20 | 436 | AMY3D_ORYSJ RecName: Full=Alpha-amylase isozyme 3D; AltName: Full=1,4-alpha-D-glucan glucanohydrolase; Flags: Precursor |
Annotations - PDB Download unfiltered results here | |||||||
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Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
PDB | 1bg9_A | 0 | 25 | 423 | 1 | 402 | A Chain A, Raphanus Sativus Anionic Peroxidase. |
PDB | 1ava_B | 0 | 25 | 423 | 1 | 402 | A Chain A, Amy2BASI PROTEIN-Protein Complex From Barley Seed |
PDB | 1ava_A | 0 | 25 | 423 | 1 | 402 | A Chain A, Amy2BASI PROTEIN-Protein Complex From Barley Seed |
PDB | 1amy_A | 0 | 25 | 423 | 1 | 402 | A Chain A, Amy2BASI PROTEIN-Protein Complex From Barley Seed |
PDB | 1rpk_A | 0 | 25 | 423 | 2 | 404 | A Chain A, Amy2BASI PROTEIN-Protein Complex From Barley Seed |
Metabolic Pathways | |||
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Pathway Name | Reaction | EC | Protein Name |
starch degradation I | RXN-1823 | EC-3.2.1.1 | α-amylase |
starch degradation I | RXN-1825 | EC-3.2.1.1 | α-amylase |